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HIGHDEF® IHC fluoromount

Permanent, high stability mounting medium for high definition visualization of IHC.
ADI-950-260-0025 25 ml Inquire for pricing
Do you need bulk/larger quantities?
  • Aqueous-based permanent mounting medium designed for fluorogenic labeling and ideal for long-term storage of processed tissue
  • High definition visualization for IHC, IF, ISH, FISH
HIGHDEF® IHC fluoromount is for mounting tissue specimens stained with fluorescent dyes. Designed for use in fluorescence, but can be used with other applications.

Product Details

Formulation:Liquid. Mounting medium containing <0.1% sodium azide.
Applications:IF, IHC
ISH (in situ hybridization)
Long Term Storage:Ambient
Technical Info/Product Notes:HIGHDEF® IHC fluoromount is an aqueous-based mounting medium for applying coverslips to tissues stained with fluorescent dyes. This product is compatible with FITC, phycoerythrin, phycocyanin, and allophycocyanin. It can also be used with other fluorescent markers such as Texas Red and rhodamine.
Protocol:Mounting Procedure:
  1. Remove the red tip from the bottle and cut the tip off with a sharp razor or scissors. This will allow smooth flow of HIGHDEF® IHC fluoromount and will prevent formation of bubbles.
  2. Place the bottle upside down in a container. This will help clear HIGHDEF® IHC fluoromount of tiny bubbles.
  3. Blot excess water from the slide without letting tissue specimens dry. Tissue should be wet prior to mounting.
  4. Apply 2-3 drops of HIGHDEF® IHC fluoromount on the tissue section and gently apply a cover slip. Allow HIGHDEF® IHC fluoromount to dry for 30-45 minutes at room temperature. For long term storage, seal coverslip edges with nail polish
Removal of Fluoromount: HIGHDEF® IHC fluoromount is an aqueous-based mounting medium and can easily be removed by soaking slides in deionized water. Place a slide in a beaker full of deionized water on a magnetic stirrer. Allow the slide to soak for few hours (or overnight) with gentle stirring for complete removal of dry HIGHDEF® IHC fluoromount.
Regulatory Status:RUO - Research Use Only

Product Literature References

Preparation of human decellularized peripheral nerve allograft using amphoteric detergent and nuclease: J. Bae, et al.; Neural Regen. Res. 9, 1890 (2021), Abstract;
Clinical Significance of CLDN18.2 Expression in Metastatic Diffuse-Type Gastric Cancer: S. Kim, et al.; J. Gastric Cancer 20, 408 (2020), Abstract;
CD56 + CD57 + Infiltrates as the Most Predominant Subset of Intragraft Natural Killer Cells in Renal Transplant Biopsies With Antibody-Mediated Rejection: H.R. Jung, et al.; Sci. Rep. 9, 16606 (2019), Abstract;
HADC8 inhibitor WK2-16 therapeutically targets lipopolysaccharide-induced mouse model of neuroinflammation and microglial activation: F.L. Lin, et al.; Int. J. Mol. Sci. 20, 410 (2019), Abstract; Full Text
Immunoprofiling of Colitis-associated and Sporadic Colorectal Cancer and its Clinical Significance: J.S. Soh, et al.; Sci. Rep. 9, 6833 (2019), Abstract; Full Text
Paired whole exome and transcriptome analyses for the Immunogenomic changes during concurrent chemoradiotherapy in esophageal squamous cell: S. Park, et al.; J. Immunother. Cancer 7, 128 (2019), Abstract; Full Text
Evaluation of lauroyl-gemcitabine-loaded hydrogel efficacy in glioblastoma rat models: C. Bastiancich, et al.; Nanomedicine (Lond.) 13, 1999 (2018), Abstract;
In vivo characterization of metabolic activity and oxidative stress in grafted human ovarian tissue using microdialysis: L. Cacciottola, et al.; Fertil. Steril. 110, 534 (2018), Abstract;
Poor prognosis in Epstein-Barr virus-negative gastric cancer with lymphoid stroma is associated with immune phenotype: C.J. Cho, et al.; Gastric Cancer 21, 925 (2018), Abstract;
Theissenolactone C exhibited ocular protection of endotoxin-induced uveitis by attenuating ocular inflammatory responses and glial activation: F.L. Lin, et al.; Front. Pharmacol. 9, 326 (2018), Abstract; Full Text
The natural retinoprotectant chrysophanol attenuated photoreceptor cell apoptosis in an N-methyl-N-nitrosourea-induced mouse model of retinal degenaration: F.L. Lin, et al.; Sci. Rep. 7, 41086 (2017), Abstract; Full Text

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