The IHC wash buffer is a for washing and removing excess reagents during immunostaining.
Product Details
| Formulation: | Liquid. 10X wash buffer. |
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| Applications: | IHC
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | Ambient |
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| Scientific Background: | Optimal immunostaining not only depends on the specificity of the primary antibody and other immunoreagents but also depends on obtaining a good signal to noise ratio. Binding of an antibody to its epitopes involves van der Waals forces, electrostatic forces and hydrophobic forces. Certain antibodies have tendency to bind loosely and nonspecifically to unrelated epitopes, which can create undesired background staining.In order to remove these nonspecifically bound antibodies, a thorough washing is required after each immunostaining step. The IHC wash buffer is specifically designed to remove such loosely bound antibodies effectively and efficiently and to provide a cleaner background staining. |
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| Technical Info/Product Notes: | Interpretation of the results will be the sole responsibility of the user. |
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| Protocol: | Preparation of Reagent: Dilute one part 10x IHC wash buffer with 9 parts deionized or distilled water.
Procedure: After each immunostaining step, wash slide(s) with 1x IHC wash buffer three times. Remove excess buffer from slide and proceed to the next immunostaining step. |
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| Regulatory Status: | RUO - Research Use Only |
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