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HIGHDEF® IHC enhancer (DAB, plus)

 
ADI-950-182-0015 15 ml Inquire for pricing
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The HIGHDEF® IHC enhancer (DAB, plus) is a DAB signal enhancer that can be used to increase intensity of immunostaining utilizing DAB as chromogen.

Product Details

Formulation:Liquid. 1X DAB chromogen enhancer.
 
Appearance:Amber solution
 
Applications:IHC
 
Shipping:Ambient
 
Long Term Storage:Ambient
 
Technical Info/Product Notes:One of the hallmarks of successful IHC is the strength of the signal generated during immunostaining. DAB, widely used as a chromogen for immunoperoxidase staining, has been well accepted by pathologists due to its superior performance compared to amino ethyl carbazol (AEC). Tissue specimens stained with DAB after only a brief incubation show increased staining intensity when the enhancer is applied. Increased sensitivity of the detection system allows the cost-effective option of diluting the primary antibody 5 to 10 fold. HIGHDEF® IHC enhancer (DAB, plus) increases the signal intensity without creating any background staining.
 
Protocol:
  1. Follow standard IHC protocol.
  2. After incubating sections with DAB reagent, rinse slides with distilled water.
  3. Apply HIGHDEF® IHC enhancer (DAB, plus) solution for 60 seconds.
  4. Rinse in buffer and continue with counterstaining.
 
Regulatory Status:RUO - Research Use Only
 

Product Literature References

Epizootics due to Yellow Fever Virus in São Paulo State, Brazil: viral dissemination to new areas (2016–2017): M.S. Cunha, et al.; Sci. Rep. 9, 5474 (2019), Abstract; Full Text

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