Replaces Prod. #: BML-AK136
- Efficient - only 50 µl diluted sample needed, saving your precious sample
- Sensitive - detect as little as 0.625 µmol/L
- Trusted - widely cited in peer-reviewed publications
- Fast - results from up to 80 samples in duplicate in just 40 minutes
The Nitric Oxide (total), detection kit is based on the enzymatic conversion of nitrate to nitrite by the enzyme nitrate reductase, followed by the Griess reaction to form a colored azo dye product. Quantification is performed by measuring absorption at 540-570 nm. Only 0.050 ml diluted sample needed per well saves precious sample.
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Product Details
| Sensitivity: | 0.625 µmol/L (range 3.125 - 100 µmol/L) |
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| Applications: | Colorimetric detection
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| Application Notes: | For the quantitative determination of total Nitric Oxide in culture supernatants, plasma, serum, saliva, and urine from any species. Cited sample type includes tissue (ref). |
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| Species reactivity: | Species independent
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| Use/Stability: | Store all components at 4°C, except nitrate reductase at -20°C. |
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| Shipping: | Blue Ice |
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| Contents: | Microtiter plate, Nitrate Reductase, Nitrate Reductase Storage Buffer, NADH, Nitrate Standard, Reaction buffer concentrate, Griess Reagent I, Griess Reagent II |
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| Scientific Background: | Nitric oxide (NO) is a major mammalian secretory product that initiates host defense, homeostatic and developmental functions by either direct effect or intercellular signaling. NO is the product of a five-electron oxidation of the amino acid L-arginine mediated by nitric oxide synthase. As a direct effector, NO is thought to activate regulatory proteins, kinases and proteases that are directed by reactive oxygen intermediates. Activation of the immune system is associated with an increase in macrophage NO production. Soluble NO exerts a variety of homeostatic influences as an activator of soluble guanylyl cyclase, a neuronal potentiator, a peripheral nervous system neurotransmitter, and a contraction regulator of both smooth muscle and vascular tissue. In addition, NO has been linked to the formation of olfactory and synaptic memories and remodeling. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
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Exploring the therapeutic efficacy of Chlorella pyrenoidosa peptides in ameliorating Alzheimer’s disease: S.M. Wang, et al.; Heliyon
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CD147 Levels in Blood and Adipose Tissues Correlate with Vascular Dysfunction in Obese Diabetic Adults: M.M. Ali, et al.; J. Cardiovasc. Dev. Dis.
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Human M1 macrophages express unique innate immune response genes after mycobacterial infection to defend against tuberculosis: A. Karshan, et al.; Commun. Biol.
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Simulating In Vitro the Bone Healing Potential of a Degradable and Tailored Multifunctional Mg-Based Alloy Platform: V. Martin, et al.; Bioengineering (Basel)
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Toxicity response to benzo[α]pyrene exposure: Modulation of immune parameters of the bay scallop, Argopecten irradians: J.A. Song, et al.; Fish Shellfish Immunol.
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Mesenchymal stem cells prevent the progression of diabetic nephropathy by improving mitochondrial function in tubular epithelial cells: S.E. Lee, et al.; Exp. Mol. Med.
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Effects of glutathione s-transferase (GST) M1 and T1 polymorphisms on antioxidant vitamins and oxidative stress-related parameters in Korean subclinical hypertensive subjects after kale juice (Brassica oleracea acephala) supplementation: H.J. Lee, et al.; Nutr. Res. Pract.
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Intermittent versus constant aerobic exercise in middle-aged males: acute effects on arterial stiffness and factors influencing the changes: D. Peres, et al.; Eur. J. Appl. Physiol.
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Resveratrol treatment may preserve the erectile function after radiotherapy by restoring antioxidant defence mechanisms, SIRT1 and NOS protein expressions: T.E. Sener, et al.; Int. J. Impot. Res.
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Clinical and biochemical data of endothelial function in Women Consuming Combined Contraceptives: I.I. Lobysheva, et al.; Data Brief
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Ghrelin alleviates paclitaxel-induced peripheral neuropathy by reducing oxidative stress and enhancing mitochondrial anti-oxidant functions in mice: N. Ishii, et al.; Eur. J. Pharmacol.
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Heme-nitrosylated hemoglobin and oxidative stress in women consuming combined contraceptives. Clinical application of the EPR spectroscopy: I.I. Lobysheva, et al.; Free Radic. Biol. Med.
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Nanoparticle mediated PPARγ gene delivery on dental implants improves osseointegration via mitochondrial biogenesis in diabetes mellitus rat model: Y. H. Lee, et al.; Nanomedicine
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Abstract;
1-Oleyl-lysophosphatidic acid (LPA) promotes polarization of BV-2 and primary murine microglia towards an M1-like phenotype: I. Plastira, et al.; J. Neuroinflammation
13, 205 (2016),
Application(s): iNOS activity assessment (through measuring accumulated total nitrate levels) primary microglia and BV-2 cell supernatant,
Abstract;
Full Text
Neuronal NOS Activates Spinal NADPH Oxidase 2 Contributing to Central Sigma-1 Receptor-Induced Pain Hypersensitivity in Mice: S.R. Choi, et al.; Biol. Pharm. Bull.
39, 1922 (2016),
Abstract;
Peroxiredoxin 6 expression is inversely correlated with nuclear factor-κB activation during Clonorchis sinensis infestation: J.H. Pak, et al.; Free Radic. Biol. Med.
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Application(s): Measuring nitrite/nitrate concentration in culture medium,
Abstract;
3-Dimensional spatially organized PEG-based hydrogels for an aortic valve co-culture model: D.S. Puperi, et al.; Biomaterials
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Application(s): Nitric oxide levels in culture supernatants,
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Analysis of interactions of nitric oxide and polyamine under cadmium stress in wheat: F. Mutlu, et al. ; Turk. J. Botany
39, 778 (2015),
Application(s): Determination of NO content in leaf tissues,
Full Text
Biochemical findings on cisplatin-induced oxidative neurotoxicity in rats: R.M. Hashem, et al.; Int. J. Adv. Res.
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Application(s): Determination of total nitric oxide (NO) in brain homogenate,
Full Text
Circulating miR-21 and eNOS in subclinical atherosclerosis in patients with hypertension: M. Cengiz, et al.; Clin. Exp. Hypertens.
37, 643 (2015),
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Comparison between the Protective Effects of Famotidine, Ginger and Marshmallow on Pyloric Ligation-Induced Peptic Ulcer in Rats: S.S. Zaghlool, et al.; J. Bioequiv. Availab.
7, 4 (2015),
Application(s): Detection in rat gastric mucosal homogenate,
Full Text
Direct Endothelial Nitric Oxide Synthase Activation Provides Atheroprotection in Diabetes-Accelerated Atherosclerosis: A. Sharma, et al.; Diabetes
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Application(s): Nitric oxide levels in human plasma,
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Endothelial damage in white coat hypertension: role of lectin-like oxidized low-density lipoprotein-1: S. Yavuzer, et al.; J. Hum. Hypertens.
29, 92 (2015),
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Interaction between pro-inflammatory cytokines and brain oxidative stress biomarkers of khat, cathinone and pseudoephedrine hydrochloride intoxication in male mice: E.H. Ali, et al.; Afr. J. Pharm. Pharmacol.
9, 585 (2015),
Application(s): ELISA using mouse brain supernatant,
Full Text
Nitric Oxide is Involved in Ibuprofen Preemptive Analgesic Effect in the Plantar Incisional Model of Postsurgical Pain in Mice: S.S. Saad, et al.; Neurosci. Lett.
614, 33 (2015),
Application(s): Measurement of nitric oxide in the spinal cord of mice,
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Soluble Lectin-Like Oxidized LDL Receptor 1 as a Possible Mediator of Endothelial Dysfunction in Patients With Metabolic Syndrome: S. Civelek, et al.; J. Clin. Lab. Anal.
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Application(s): Analysis of MetS in humans ,
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Total oxidant and antioxidant capacities, nitric oxide and malondialdehyde levels in cats seropositive for the feline coronavirus: A. Kayar, et al.; Vet. Med. (Praha)
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Application(s): Analysis in cats seropositive for the feline coronavirus ,
Full Text
Vascular toxicity of ultra-small TiO2 nanoparticles and single walled carbon nanotubes in vitro and in vivo: N. Bayat, et al.; Biomaterials
63, 1 (2015),
Abstract;
Zinc Oxide Nanoparticles Induce Lipoxygenase-mediated Apoptosis and Necrosis in Human Neuroblastoma SH-SY5Y Cells: J.H. Kim, et al.; Neurochem. Int.
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Application(s): Detection of total NO in human neuroblastoma,
Abstract;
Biomimetic collagenous scaffold to tune inflammation by targeting macrophages: F. Taraballi, et al.; J. Tissue Eng.
7, 2041731415624667 (2014),
Application(s): Presence of nitric oxide (NO; µM) in the culture supernatants,
Abstract;
Full Text
Control of disease tolerance to malaria by nitric oxide and carbon monoxide: V. Jeney, et al.; Cell Rep.
8, 126 (2014),
Application(s): Injection of DPTA-NO and assessment with NO Detection Kit,
Abstract;
Moderate intensity physical activity prevents increased blood glucose concentrations, fat pad deposition and cardiac action potential prolongation following diet-induced obesity in a juvenile-adolescent rat model: A. van Waveren, et al.; BMC Obes.
1, 11 (2014),
Abstract;
Full Text
Chronic estradiol-17β exposure suppresses hypothalamic norepinephrine release and the steroid-induced luteinizing hormone surge: role of nitration of tyrosine hydroxylase: B.S. Kasturi, et al.; Brain Res.
1493, 90 (2013),
Application(s): NO detected in rat brain tissue homogenates,
Abstract;
Enhanced K(+) secretion in dextran sulfate-induced colitis reflects upregulation of large conductance apical K(+) channels (BK; Kcnma1).: B.M. Kanthesh, et al.; Am. J. Physiol. Cell. Physiol.
305, C972 (2013),
Application(s): Nitric oxide production in rat distal colon homogenates,
Abstract;
Full Text
TXNIP deficiency exacerbates endotoxic shock via the induction of excessive nitric oxide synthesis: Y. Park, et al.; PLoS Pathog.
9, e1003646 (2013),
Application(s): Assay using mouse serum, liver, lung, and spleen samples ,
Abstract;
Full Text
Chronic estradiol exposure induces oxidative stress in the hypothalamus to decrease hypothalamic dopamine and cause hyperprolactinemia: S.M. MohanKumar, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol.
300, R693 (2011),
Application(s): NO detected in rat brain tissue homogenates,
Abstract;
Full Text
Acylated and Unacylated Ghrelin Promote Proliferation and Inhibit Apoptosis of Pancreatic {beta}-Cells and Human Islets: Involvement of 3',5'-Cyclic Adenosine Monophosphate/Protein Kinase A, Extracellular Signal-Regulated Kinase 1/2, and Phosphatidyl Inositol 3-Kinase/Akt Signaling: R. Granata, et al. ; Endocrinology
148, 512 (2007),
Application(s): EIA using hamster & rat culture supernatants,
Abstract;
Endocrine Effects of Relaxin Overexpression in Mice: S. Feng, et al. ; Endocrinology
147, 407 (2006),
Application(s): EIA using mouse plasma,
Abstract;
Fluid shear stress-induced nitric oxide production in human cavernosal endothelial cells: inhibition by hyperglycaemia: H. Wessells, et al. ; BJU Int.
97, 1047 (2006),
Application(s): EIA using human culture supernatants,
Abstract;
In Vivo Characterization of the Novel Imidazopyridine BYK191023 [2-[2-(4-Methoxy-pyridin-2-yl)-ethyl]-3H-imidazo[4,5-b]pyridine], a Potent and Highly Selective Inhibitor of Inducible Nitric-Oxide Synthase: M.D. Lehner, et al. ; J. Pharmacol. Exp. Ther.
317, 181 (2006),
Application(s): EIA using rat plasma,
Abstract;
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