Product Details
| Alternative Name: | Phosphatidylinositol 4,5-bisphosphate, PtdIns(4,5)P2 |
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| Clone: | KT10 |
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| Host: | Mouse |
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| Isotype: | IgG2b |
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| Immunogen: | Native PIP2 from bovine spinal cord. |
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| Source: | Purified from mouse ascites. |
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| Species reactivity: | Species independent
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| Applications: | ELISA, ICC
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In PBS containing 0.05% sodium azide. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Metabolism of inositol phospholipids by intracellular signaling mediators is fundamental to signal transduction in eukaryotic cells. PI-4,5-P2 (Phosphatidylinositol 4,5-bisphosphate; PIP2) can be synthesized by phosphorylation of PI-4-P by type I phosphatidylinositol phosphate kinase (PIP5K I), or phosphorylation of PI-5-P by type II PIPK (PIP4K II). PI-4,5-P2 regulation of cellular calcium levels involves its hydrolysis by Phospholipase C (PLC) to produce inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG), which serve as second messengers in the import of calcium via IP3-sensitive ion channels and in the activation of PKC, respectively. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
An acquired phosphatidylinositol 4-phosphate transport initiates T-cell deterioration and leukemogenesis: W. Zhong, et al.; Nat. Commun.
13, 4390 (2022),
Abstract;
Distribution profile of inositol 1,4,5-trisphosphate receptor/Ca2+ channels in α and β cells of pancreas: dominant localization in secretory granules and common error in identification of secretory granule membranes: Y.S. Hur, et al.; Pancreas
44, 158 (2015),
Abstract;
MARCKS regulates membrane targeting of Rab10 vesicles to promote axon development: X.H. Xu, et al.; Cell. Res.
24, 576 (2014),
Application(s): Immunofluorescence,
Abstract;
Phosphatidylinositol 4,5-bisphosphate alters the number of attachment sites between ezrin and actin filaments: a colloidal probe study: J.A. Braunger, et al.; J. Biol. Chem.
289, 9833 (2014),
Application(s): Immunofluorescence,
Abstract;
PIP2 regulates psychostimulant behaviors through its interaction with a membrane protein: P.J. Hamilton, et al.; Nat. Chem. Biol.
10, 582 (2014),
Application(s): Immunoprecipitation,
Abstract;
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PIP2 controls voltage-sensor movement and pore opening of Kv channels through the S4-S5 linker: A.A. Rodriguez-Menchaca, et al.; PNAS
109, E2399 (2012),
Abstract;
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