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Methyltransferase activity kit

ADI-907-025 96 wells 529.00 USD
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  • Universal - suitable for any AdoMet-dependent methyltransferase
  • Robust - Z factor = 0.83
  • Sensitive - fluorescent detection allows for both DNA and protein assays
  • Higher throughput - homogeneous mix-and-read assay
The Methyltransferase activity kit is a homogeneous mix-and-read fluorescent assay for the determination of any S-adenosyl-L-methionine (AdoMet/SAM) dependent methyltransferase activity. It is suitable for end-point or kinetic read options, which is ideal for determining mechanism of action, kinetics, and screening candidate compounds. The assay is amendable to HTS and miniaturization.
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Product Details

Applications:Fluorescent detection, HTS
Activity assay
Application Notes:For the measurement of AdoMet-dependent methyltransferase activity in purified in vitro samples that do not contain reductants from any species.
Species reactivity:Species independent
Use/Stability:Store assay buffer +4° and all other components at -20°.
Shipping:Shipped on Blue Ice
Contents:Black Microtiter plate, Reaction buffer, Assay buffer, Detection solution, Positive control
Scientific Background:The methylation of proteins, xenobiotic drugs, nucleic acids, and oligosaccharides is known to regulate important cellular events including meiosis, biosynthesis, development, signal transduction, chromatin remodeling, and gene silencing. The most frequent methyl donor for this type of enzymatic modification is S-adenosyl-L-methionine (AdoMet/SAM). Based on the predicted binding motif for AdoMet, it is estimated that 1% of the mammalian genome encodes a variety of substrate-specific methyltransferases.
Regulatory Status:RUO - Research Use Only

Product Literature References

High yield recombinant expression and purification of oncogenic NSD1, NSD2, and NSD3 with human influenza hemagglutinin tag: Y. Shen, et al.; Protein Expr. Purif. 166, 105506 (2020), Abstract;
Identification of LEM-14 inhibitor of the oncoprotein NSD2: Y. Shen, et al.; Biochem. Biophys. Res. Commun. 508, 102 (2019), Abstract;
Set7 Is a H3K37 methyltransferase in Schizosaccharomyces pombe and is required for proper gametogenesis: Y. Shen, et al.; Structure 27, 631 (2019), Abstract;
Cyclic-di-GMP regulates lipopolysaccharide modification and contributes to Pseudomonas aeruginosa immune evasion: R.R. McCarthy, et al.; Nat. Microbiol. 2, 17027 (2017), Abstract; Full Text
Lipotropes protect against pathogen-aggravated stress and mortality in low dose pesticide-exposed fish: N. Kumar, et al.; PLoS One 9, e93499 (2014), Abstract; Full Text
Epigenetic upregulation of Bak by ZBP-89 inhibits the growth of hepatocellular carcinoma: C.G. Ye, et al.; Biochim. Biophys. Acta 1833, 2970 (2013), Abstract; Full Text
Protein kinase C stimulates HuD-mediated mRNA stability and protein expression of neurotrophic factors and enhances dendritic maturation of hippocampal neurons in culture: C.S. Lim, et al.; Hippocampus 22, 2303 (2012), Abstract;
Methyl-and acetyltransferases are stable epigenetic markers postmortem: C.M. Monoranu, et al.; Cell Tissue Bank. 12, 289 (2011), Abstract;
Identification of a methyltransferase encoded by gene stel16 and its function in Ebosin biosynthesis of Streptomyces sp. 139: H.G. Xie, et al.; J. Microbiol. 47, 193 (2009), Abstract;

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