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Higher throughput - measure caspase-3 activity in up to 37 samples in duplicate
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Efficient - only 50 µl diluted sample needed per well
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Flexible - kinetic or end-point assay option
The Caspase-3 colorimetric detection kit provides a higher throughput method for measuring caspase-3 activity in up 37 samples in duplicate per kit. The kit is flexible, providing both kinetic or end-point assay options.
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Product Details
| Alternative Name: | CPP32, Yama, Apopain |
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| Sensitivity: | 16.4 Units/ml (range 62.5 - 1000 Units/ml) |
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| Applications: | Colorimetric detection, HTS
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| Application Notes: | For the colorimetric measurement of Caspase-3 in cell lysates and buffers from any species. |
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| Species reactivity: | Species independent
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| Use/Stability: | Store all components at 4°C, except standard at -20°C. |
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| Shipping: | Blue Ice |
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| Contents: | Half-Area Microtiter plate, Standard, DTT, p-Nitroaniline Calibrator, Reaction buffer concentrate, Substrate concentrate, Stop solution 2 |
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| Scientific Background: | The caspases are a family of cysteine proteases that cleave after certain aspartate residues, and are primarily recognized as mediators of apoptosis. caspases are synthesized as inactive zymogens that can be cleaved to form active enzymes following the induction of apoptosis by stress or death receptors. Initiator caspases (e.g. caspase-8 and -10) are activated by dimerization of the zymogen on a dedicated adaptor protein. These activated initiator caspases in-turn cleave downstream effector or “executioner” caspases (e.g. caspase-3, -6, and -7) in a cascade-like manner, which cleave key cellular proteins that lead to the morphological changes associated with apoptotic cell death. |
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| Technical Info/Product Notes: | Biocompare Article: Keep an Eye on Apoptosis with Caspase Assays |
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| UniProt ID: | P42574 |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Synergistic effects of pomegranate juice and atorvastatin for improving cerebellar structure and function of breast-feeding rats maternally fed on a high cholesterol diet: H.I.H. EL-Sayyad, et al.; J. Chem. Neuroanat.
24, 101798 (2020),
Abstract;
Cadmium nitrate-induced neuronal apoptosis is protected by N-acetyl-l-cysteine via reducing reactive oxygen species generation and mitochondria dysfunction: C.Y. Lee, et al.; Biomed. Pharmacother.
108, 448 (2018),
Abstract;
Zileuton alleviates acute cisplatin nephrotoxicity: Inhibition of lipoxygenase pathway favorably modulates the renal oxidative/inflammatory/caspase-3 axis: M.M. Helmy, et al.; Prostaglandins Other Lipid Mediat.
135, 1 (2018),
Abstract;
Dietary Pyridoxine Protects against Stress and Maintains Immunohaematological Status in Chanos chanos Exposed to Endosulfan: N. Kumar, et al.; Basic Clin. Pharmacol. Toxicol.
119, 297 (2016),
Abstract;
Obestatin promotes proliferation and survival of adult hippocampal progenitors and reduces amyloid-β-induced toxicity: E. Gargantini, et al.; Mol Cell Endocrinol.
422, 18 (2016),
Application(s): Caspase-3 activity for apoptosis assessment,
Abstract;
Antioxidant and antiapoptotic effects of darbepoetin-α against traumatic brain injury in rats: H. Kertmen, et al.; Arch. Med. Sci.
11, 1119 (2015),
Application(s): Tissue caspase-3 analysis,
Abstract;
Full Text
Celastrol prevents circulatory failure via induction of heme oxygenase-1 and heat shock protein 70 in endotoxemic rats: Y.L. Wang, et al.; J. Ethnopharmacol.
162, 168 (2015),
Application(s): Assay,
Abstract;
Lipotropes protect against pathogen-aggravated stress and mortality in low dose pesticide-exposed fish: N. Kumar, et al.; PLoS One
9, e93499 (2014),
Abstract;
Full Text
The protective effect of 2-mercaptoethane sulfonate (MESNA) against traumatic brain injury in rats: E.R. Yilmaz, et al.; Acta Neurochir.
155, 141 (2013),
Abstract;
Activation of the P2Y1 receptor induces apoptosis and inhibits proliferation of prostate cancer cells: Q. Wei, et al.; Biochem. Pharmacol.
82, 418 (2011),
Application(s): Caspase-3 activity measured in human prostatic carcinoma (PC-3) lysate,
Abstract;
Full Text
Arsenic trioxide inhibits cholangiocarcinoma cell growth and induces apoptosis: F. Zhong, et al.; Pathol. Oncol. Res.
16, 413 (2010),
Abstract;
Cilostazol enhances apoptosis of synovial cells from rheumatoid arthritis patients with inhibition of cytokine formation via Nrf2-linked heme oxygenase 1 induction: Y.S. Park, et al.; Arthritis Rheum.
62, 732 (2010),
Application(s): Caspase-3 activity measured in human synovial cell lysate,
Abstract;
Full Text
Growth hormone expression in stromal and non-stromal cells in the bursa of Fabricius during bursal development and involution: Causal relationships?: A.J. Rodríguez-Méndez, et al.; Gen. Comp. Endocrinol.
167, 297 (2010),
Abstract;
Neuroprotective effect of mesna (2-mercaptoethane sulfonate) against spinal cord ischemia/reperfusion injury in rabbits: H. Dolgun, et al.; J. Clin. Neurosci.
17, 486 (2010),
Abstract;
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