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sPLA2 activity kit

ADI-907-002 96 wells 547.00 USD
Do you need bulk/larger quantities?
  • Higher throughput - assay up to 42 samples in duplicate per 96 well kit
  • Sensitive - detect < 3 U/ml
  • Fast - results in just 40 minutes!
The sPLA2 activity kit is a higher throughput assay for the quantification of up to 42 samples in duplicate per 96 well kit. This kit provides safe, sensitive, and reliable results in under an hour.
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Product Details

Alternative Name:Secretory phospholipase A2
Sensitivity:2.34 Units/ml (range 5 - 80 Units/mL)
Applications:Colorimetric detection
Activity assay
Application Notes:For the quantitative determination of sPLA2 in culture supernatants, cell lysates, and buffers from any species.
Use/Stability:Store all components 4°C, except Substrate at -20°C.
Shipping:Blue Ice
Contents:Microtiter plate, Standard, Reaction buffer concentrate, Substrate, Stop reagent, Color reagent
Scientific Background:Secretory Phospholipase A2s (sPLA2s) are a group of related enzymes that upon activation, cleave an acyl ester bond in the sn-2 position in glycerophospholipid. These are extracellular enzymes that have high disulfide bond content, low molecular mass (14KDa), and require mM levels of Ca+ for catalysis. sPLA2 enzymes play a crucial role in the generation of arachidonic acid, which is further metabolized to proinflammatory eicosanoids.
Regulatory Status:RUO - Research Use Only
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

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Product Literature References

Infection of Galleria mellonella (lepidoptera) larvae with the entomopathogenic fungus Conidiobolus coronatus (entomophthorales) induces apoptosis of hemocytes and affects the concentration of eicosanoids in the hemolymph: A.K. Wronska, et al.; Front. Physiol. 12, 774086 (2022), Application(s): Galleria mellonella larval hemolymph, Abstract; Full Text
Club cell protein 16 (Cc16) deficiency increases inflamm-aging in the lungs of mice: M.E. Laucho-Contreras, et al.; Physiol. Rep. 6, e13797 (2018), Abstract; Full Text

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