- Higher throughput - assay up to 42 samples in duplicate per 96 well kit
- Sensitive - detect < 3 U/ml
- Fast - results in just 40 minutes!
The sPLA2 activity kit is a higher throughput assay for the quantification of up to 42 samples in duplicate per 96 well kit. This kit provides safe, sensitive, and reliable results in under an hour.
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Product Details
| Alternative Name: | Secretory phospholipase A2 |
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| Sensitivity: | 2.34 Units/ml (range 5 - 80 Units/mL) |
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| Applications: | Colorimetric detection
Activity assay
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| Application Notes: | For the quantitative determination of sPLA2 in culture supernatants, cell lysates, and buffers from any species. |
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| Use/Stability: | Store all components 4°C, except Substrate at -20°C. |
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| Shipping: | Shipped on Blue Ice |
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| Contents: | Microtiter plate, Standard, Reaction buffer concentrate, Substrate, Stop reagent, Color reagent |
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| Scientific Background: | Secretory Phospholipase A2s (sPLA2s) are a group of related enzymes that upon activation, cleave an acyl ester bond in the sn-2 position in glycerophospholipid. These are extracellular enzymes that have high disulfide bond content, low molecular mass (14KDa), and require mM levels of Ca+ for catalysis. sPLA2 enzymes play a crucial role in the generation of arachidonic acid, which is further metabolized to proinflammatory eicosanoids. |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Infection of Galleria mellonella (lepidoptera) larvae with the entomopathogenic fungus Conidiobolus coronatus (entomophthorales) induces apoptosis of hemocytes and affects the concentration of eicosanoids in the hemolymph: A.K. Wronska, et al.; Front. Physiol.
12, 774086 (2022),
Application(s): Galleria mellonella larval hemolymph,
Abstract;
Full Text
Club cell protein 16 (Cc16) deficiency increases inflamm-aging in the lungs of mice: M.E. Laucho-Contreras, et al.; Physiol. Rep.
6, e13797 (2018),
Abstract;
Full Text
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