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PGF receptor (FP) polyclonal antibody

ADI-905-901-100 100 µg 441.00 USD
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Product Details

Alternative Name:PTGFR, Prostaglandin F2α receptor
Immunogen:Synthetic peptide corresponding to the sequence near the N-terminus of rat PGF2 Receptor.
UniProt ID:P43118
Species reactivity:Mouse, Rat
Crossreactivity:Predicted species reactivity based on epitope sequence homology: wild boar, dog.
Applications:ELISA, IHC, WB
Application Notes:Suitable for membrane-based ELISA.
Predicted MW of ~41kDa, observed by Western blot ~45kDa. Higher MW species (~60kDa) and intermediates also observed by Western blot which reflect post-translational modification of the receptor.
Purity Detail:Peptide affinity purified.
Formulation:Liquid. In PBS containing 50% glycerol and 0.01% sodium azide.
Handling:Keep on ice at all times.
Shipping:Blue Ice
Long Term Storage:-20°C
Scientific Background:Arachidonic acid is converted by cyclooxygenase (COX) to prostaglandin H2 (PGH2), which is further converted to the derivative prostanoids PGD2, PGE2, PGF2α, PGI2 (prostacyclin) and thromboxane A2 (TXA2). Five classes of prostanoid receptors are recognized and named DP, EP, FP, IP and TP receptors corresponding to the agonist properties of the associated prostanoids. FP receptors are found in smooth muscle where they mediate contraction, and are believed to be coupled via Gq/11 subunits to stimulate phosphatidyl inositol (PI) hydrolysis. The FP agonist Fluprostenol (a PGF2α analogue) is used in animal husbandry to synchronize oestrus and induce partruition. FP receptors are known to mediate intraocular pressure, and FP agonists such as latanoprost and travoprost are drugs commonly use to treat glaucoma.
Regulatory Status:RUO - Research Use Only
Manufacturer:Manufactured by Proteimax.
PGF2α receptor (FP) polyclonal antibody Western blot
Western blot analysis of MW marker (1) and 25µg rat kidney extract (2), probed with PGF2α Receptor (FP) pAb.
PGF2α receptor (FP) polyclonal antibody Membrane ELISA
Membrane ELISA: Lewis rat striatum membranes (5µg/well) were treated with 1 μM concentrations of agonist (cyclosin) and probed with PGF2α Receptor (FP) pAb (1:250 to 1:2000 of a 1µg/μl stock solution) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=4).
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PGF2α receptor (FP) polyclonal antibody Western blot PGF2α receptor (FP) polyclonal antibody Membrane ELISA

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Prostaglandin receptor
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Prostaglandin receptor Polyclonal antibody
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