| Alternative Name: | POU5F1, POU domain class 5 transcription factor 1, OTF3/4, Octamer-binding protein 3/4 |
| |
| Clone: | 9B7 |
| |
| Host: | Mouse |
| |
| Isotype: | IgG1 |
| |
| Immunogen: | Recombinant human Oct4 (aa 1-360). |
| |
| UniProt ID: | Q01860 |
| |
| Species reactivity: | Human
|
| |
| Applications: | WB
|
| |
| Recommended Dilutions/Conditions: | Western Blot (1:5,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
| |
| Application Notes: | Detects a band of ~39kDa (full length) by Western blot. |
| |
| Purity Detail: | Protein G affinity purified. |
| |
| Formulation: | Liquid. In PBS, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
| |
| Shipping: | Shipped on Blue Ice |
| |
| Long Term Storage: | -20°C |
| |
| Scientific Background: | Oct4 is a members of Oct family of transcription factors which specifically interact with Octamer motif ATGCAAAT, a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. All of the members of the Oct family contain two highly conserved domains which are separated by 14-26 variable amino acids. These include the POU homeodomain and the POU-specific domain. Both are required for DNA binding and are involved in protein-protein interactions. Evidences indicate that regulation of Oct family transcription factors occurs at the level of phosphorylation. Oct4 is renowned as a transcription factor expressed by undifferentiated embryonic stem cells and embryonic germ cells. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
