Replaces Prod. #: ALX-804-412
Product Details
Clone: | 8G7 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Synthetic peptide corresponding to aa 1-42 of amyloid β A4. |
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UniProt ID: | P05067 |
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Species reactivity: | Human
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Crossreactivity: | Does not cross react with β A4 (1-40). |
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Applications: | ELISA, ICC, WB
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Recommended Dilutions/Conditions: | ELISA (0.05µg/ml) Immunocytochemistry (0.1-1.0µg/ml) Western Blot (1µg/ml) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects a band of ~4kDa by Western blot. |
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Purity Detail: | Thiophilic adsorption and size exclusion chromatography purified. |
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Formulation: | Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose. |
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Reconstitution: | Reconstitute with 1ml water (15 minutes at room temperature). |
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Use/Stability: | Stable at -80°C up to 1 year, at 4°C up to 3 months. |
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Shipping: | Shipped on Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | The β-amyloid peptide (β A4), proteolytically released from the amyloid precursor protein (APP), provides the principal component of senile plaques in Alzheimer's disease. Cleavage of APP by α-secretase or alternatively by β-secretase leads to generation and extracellular release of soluble APP peptides, S-APP-α and S-APP-β, respectively, and the retention of corresponding membrane-anchored C-terminal fragments, C83 and C99. Subsequent processing of C83 by γ-secretase yields P3 peptides. This is the major secretory pathway and is nonamyloidogenic. Alternatively, presenilin/ nicastrin-mediated γ-secretase processing of C99 releases the amyloid β proteins, amyloid-β 40 (Aβ40) and amyloid-β 42 (Aβ42), major components of amyloid plaques, and the cytotoxic C-terminal fragments, γ-CTF(50), γ-CTF(57) and γ-CTF(59). |
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Technical Info/Product Notes: | Application Note:
Analysis of the Effect of Aggregated β-Amyloid on Cellular Signaling Pathways Critical for Memory in Alzheimer’s disease |
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Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of (1) β A4 (1-40), (2) β A4 (1-42), and (3) β A4 (1-43), probed with 2µg/ml of Amyloid β A4 (42) mAb (8G7).
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