Product Details
| Alternative Name: | NPY1R, NPYR |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to the sequence near the N-terminus of rat neuropeptide Y receptor Y1. |
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| UniProt ID: | P21555 |
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| Species reactivity: | Human, Mouse, Rat
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| Crossreactivity: | Predicted species reactivity based on epitope sequence homology: horse, chimpanzee, rhesus macaque, guinea pig. |
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| Applications: | ELISA, WB
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| Application Notes: | Suitable for membrane-based ELISA.
Predicted MW of ~44kDa. Higher MW species (~80kDa) also observed by Western blot which may reflects post-translational modification or dimerization of the receptor. |
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| Purity Detail: | Peptide affinity purified. |
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| Formulation: | Liquid. In PBS containing 50% glycerol and 0.01% sodium azide. |
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| Handling: | Keep on ice at all times. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Neuropeptide Y (NPY) and the closely related peptide YY (PYY) and pancreatic polypeptide (PP) are a family of 36-amino acid, tyrosine-rich peptides with amidated C-terminal ends that are known to regulate gastrointestinal and central nervous system functions including food intake and the inhibition of anxiety. Four functional NPY receptors (Y1/Y2/Y4/Y5) have been identified to date, with a fifth (y6) being a non-functional receptor in primates that is absent from the rat genome. Y1, Y2, and Y5 receptors preferentially bind NPY and PYY, while the Y4 receptor preferentially binds PP. All four receptors signal via coupling to Gi/o alpha subunits, inhibiting adenylyl cyclase activity, thereby decreasing intracellular cAMP levels. Y1 receptor deficient mice display a variety of physical and behavioral phenotypes including obesity, hyperinsulinemia, and increased territorial behavior. |
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| Regulatory Status: | RUO - Research Use Only |
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| Manufacturer: | Manufactured by Proteimax. |
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Western blot analysis: MW marker (1) and 20µg rat brain extract (2) probed with Neuropeptide Y Receptor (Y1) pAb at 5.1µg/ml.
Membrane ELISA: Lewis rat heart membranes (5µg/well) were treated with 1μM concentrations of agonist (NPY) and probed with Neuropeptide Y Receptor (Y1) pAb (1:250 to 1:2000 of a 1µg/μl stock solution) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=4).
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Product Literature References
Generation of G protein-coupled receptor antibodies differentially sensitive to conformational states: A.S. Heimann, et al.; PloS One
12, e0187306 (2017),
Abstract;
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