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[pThr56]eEF2 polyclonal antibody

ADI-905-775-100 100 µg 304.00 USD
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Product Specification

Alternative Name:Eukaryotic elongation factor 2
Immunogen:Synthetic peptide corresponding to human eEF2 phosphorylated at Thr56.
UniProt ID:P13639
Source:Purified from rabbit serum.
Species reactivity:Human, Mouse, Rat
Recommended Dilutions/Conditions:Western Blot (1µg/ml, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~95kDa by Western blot.
Purity Detail:Peptide affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:Eukaryotic elongation factor 2 (eEF2) mediates the GTP-dependent translocation of nascent peptides from the A-site to the P-site of the ribosome relative to mRNA after addition of each amino acid to the nascent chain. Phosphorylation of eEF2 within the GTPbinding domain at Thr56 by eEF2 kinase inactivates eEF2 and results in the termination of mRNA translation. Conversely, eEF2-regulated protein synthesis can be stimulated by dephosphorylation of eEF2 at Thr56 by protein phosphatases such as PP2A, and/or by upstream kinase inactivation via the mTOR pathway.
Regulatory Status:RUO - Research Use Only
[pThr56]eEF2 polyclonal antibody Western blot
Western blot analysis of eEF2 (pThr56) using K-562, K-562 + Anisomycin, 3T3, and 3T3 + Anisomycin cell extracts. Lane 1: Molecular Weight Markers, Lane 2: K-562, Lane 3: K-562 + Anisomycin, Lane 4: 3T3, Lane 5: 3T3 + Anisomycin.
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[pThr56]eEF2 polyclonal antibody Western blot

Product Literature References

Coordination of changes in expression and phosphorylation of eukaryotic elongation factor 2 (eEF2) and eEF2 kinase in hypertrophied cardiomyocytes: S. Kameshima, et al.; Biochem. Biophys. Rep. 7, 218 (2016), Application(s): Western blot, Immunohistochemistry, Abstract; Full Text

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