| Clone: | 10E10 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Synthetic peptide corresponding to a portion of Raptor. |
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| UniProt ID: | Q8N122 |
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| Species reactivity: | Human, Mouse, Rat
Dog
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| Applications: | WB
|
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| Recommended Dilutions/Conditions: | Western Blot (0.5µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~160kDa by Western blot. |
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| Purity Detail: | Thiophilic adsorption and size exclusion chromatography purified. |
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| Formulation: | Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose. |
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| Reconstitution: | Reconstitute with 1ml water (15 minutes at room temperature). |
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| Use/Stability: | Stable at -80°C up to 1 year, at 4°C up to 3 months. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | The mTOR (mammalian target of rapamycin) pathway coordinates nutrient signals with growth factor dependent signaling. mTOR is organized in two independent protein complexes, mTORC1 and mTORC2. The mTORC1 complex containing mTOR, GβL/mLST8 and Raptor is sensitive to rapamycin, while the mTORC2 complex containing mTOR, GβL/mLST8, SIN1, and Rictor is rapamycin insensitive. |
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| Regulatory Status: | RUO - Research Use Only |
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