[pSer⁹]GSK-3β monoclonal antibody (3A8)



ADI-905-761-100	100 µg	501.00 USD
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Product Details

Alternative Name:	Glycogen synthase kinase-3β

Clone:	3A8

Host:	Mouse

Isotype:	IgG1

Immunogen:	Synthetic peptide corresponding to a portion of GSK-3β phosphorylated at Ser⁹.

UniProt ID:	P49841

Species reactivity:	Human, Mouse, Rat Dog

Applications:	ELISA, WB

Purity Detail:	Thiophilic adsorption and size exclusion chromatography purified.

Formulation:	Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.

Reconstitution:	Reconstitute with 1ml water (15 minutes at room temperature).

Use/Stability:	Stable at -80°C up to 1 year, at 4°C up to 3 months.

Handling:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.

Shipping:	Blue Ice

Long Term Storage:	-20°C

Scientific Background:	Glycogen Synthase Kinase 3β (GSK-3β) is a unique serine/threonine kinase that is inactivated by phosphorylation. In response to insulin binding, PKB/AKT phosphorylates GSK-3β on serine 9, which prevents GSK-3β from phosphorylating glycogen synthase. Unphosphorylated glycogen synthase is active and able to synthesize glycogen. GSK-3β is also unique in that it requires a substrate that has been phosphorylated by a distinct kinase before it can phosphorylate the substrate. This phosphate priming mechanism explains why phosphorylation of serine 9 inactivates GSK-3β. The phosphorylated serine binds to the GSK-3β priming phosphate position and prevents binding of alternative substrates. In addition to insulin signaling, GSK-3β participates in the Wnt signaling pathway, where it forms a complex with axin, beta-catenin and adenomatous polyposis coli (APC) protein. In the presence of Wnts, GSK-3β is unable to phosphorylate beta-catenin, which leads to stabilization of beta-catenin. The Wnt pathway inactivates GSK-3β via the proteins, Dishevelled and FRAT, which disrupt the interaction of GSK-3β with axin, beta-catenin, and APC. Clinically, there is considerable interest in GSK-3β inhibitors because they may mimic the effect of insulin or reduce the hyperphosphorylation of Tau that is observed in Alzheimer's Disease.

Regulatory Status:	RUO - Research Use Only

[pSer9]GSK-3β monoclonal antibody (3A8) Western blot

Western blot analysis of AY31 cells untreated (co), EGF stimulated (EGF), or pervanadate treated (VH), with GSK3β (phospho-Ser9) mAb (3A8).

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Product Literature References

α-Synuclein Binds to the ER-mitochondria Tethering Protein VAPB to Disrupt Ca2+ Homeostasis and Mitochondrial ATP Production: S. Paillusson, et al.; Acta Neuropathol. 134, 129 (2017), Abstract; Full Text