Product Details
| Alternative Name: | CB2, CNR2 |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to the sequence near the N-terminus of rat cannabinoid receptor CB2. |
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| UniProt ID: | Q9QZN9 |
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| Species reactivity: | Rat
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| Crossreactivity: | Predicted species reactivity based on epitope sequence homology: human, mouse, bovine, horse, rhesus macaque, dog, chimpanzee. |
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| Applications: | ELISA, IHC, WB
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| Application Notes: | Suitable for cell-based and membrane-based ELISA.
Predicted MW of ~35kDa. Higher MW species (~50kDa) and intermediates also observed by Western blot which reflect post-translational modification of the receptor. |
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| Purity Detail: | Peptide affinity purified. |
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| Formulation: | Liquid. In PBS containing 50% glycerol and 0.01% sodium azide. |
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| Handling: | Keep on ice at all times. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | The cannabinoid receptors CB1 and CB2 are GPCRs named for their signaling function in response to cannabinoid drugs such as Delta9-tetrahydrocannabinol (Delta9-THC). In addition to Delta9-THC and its synthetic analogs, CB1 and CB2 receptors are bound by endogenous eicosanoid ligands (e.g. anandamide), and signal via Gi/o alpha subunits to regulate adenylyl cyclase activity (generally inhibitory), ion channel activation, intracellular Ca2+ levels, nitric oxide synthase activation, and immediate early gene expression. CB1 and CB2 signals in turn regulate neurological and immunological functions such as inhibition of neurotransmitter release and immune cell migration, antigen processing, and cytokine production. |
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| Regulatory Status: | RUO - Research Use Only |
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| Manufacturer: | Manufactured by Proteimax. |
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Western blot analysis: MW marker (1) and 10µg rat brain extract (2) probed with Cannabinoid Receptor (CB2) pAb at 4µg/ml.
Membrane ELISA: Lewis rat spleen membranes (5µg/well) were treated with 1 μM concentrations of agonist (Δ9-THC) and probed with Cannabinoid Receptor (CB2) pAb (1:25 to 1:200 of a 0.1µg/μl stock solution) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=2).
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Product Literature References
Generation of G protein-coupled receptor antibodies differentially sensitive to conformational states: A.S. Heimann, et al.; PloS One
12, e0187306 (2017),
Abstract;
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