Replaces Prod. #: BML-SA633
Product Details
| Alternative Name: | B2BKR |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to the sequence near the N-terminus of rat bradykinin receptor B2. |
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| UniProt ID: | P25023 |
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| Species reactivity: | Mouse, Rat
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| Crossreactivity: | Predicted species reactivity based on epitope sequence homology: guinea pig, dog. |
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| Applications: | ELISA, IHC, WB
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| Application Notes: | Suitable for membrane-based ELISA.
Predicted MW of ~45kDa. Higher MW species (~50kDa) and intermediates also observed by Western blot which reflect post-translational modification or dimerization of the receptor. |
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| Purity Detail: | Peptide affinity purified. |
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| Formulation: | Liquid. In PBS containing 50% glycerol and 0.01% sodium azide. |
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| Handling: | Keep on ice at all times. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Bradykinin (BK) is a nine-amino acid peptide produced by kallikrein cleavage of kininogen precursor proteins in tissue and plasma, and is an important regulator of vascular and pain responses to tissue injury. Two receptors (B1 and B2) have been identified as mediators of BK signaling, and are both seven-transmembrane domain-containing GPCRs. The B2 receptor is constitutively expressed in smooth muscle and neurons, whereas B1 expression is induced following injury. B2 receptors signal via Gq alpha subunits, leading to activation of phospholipase Cbeta, hydrolysis of PI, and an intracellular increase in free calcium, although coupling through Gi, Gs, and G12/13 has also been observed. |
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| Regulatory Status: | RUO - Research Use Only |
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| Manufacturer: | Manufactured by Proteimax. |
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Membrane ELISA: Lewis rat heart membranes (5µg/well) were treated with 1 μM concentrations of agonist (BK) and probed with Bradykinin Receptor (B2) pAb (1:250 to 1:2000 of a 1µg/μL stock solution) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=3).
Immunohistochemistry analysis: Mouse brain fixed with 4% paraformaldehyde, stained with Bradykinin Receptor (B2) pAb at 1:1000, and visualized at 40x.
Western blot analysis: MW marker (1) and 20µg rat brain extract (2) probed with Bradykinin Receptor (B2) pAb at 2.2µg/ml.
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Product Literature References
Generation of G protein-coupled receptor antibodies differentially sensitive to conformational states: A.S. Heimann, et al.; PloS One
12, e0187306 (2017),
Abstract;
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