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Pax3 monoclonal antibody (C2)

ADI-905-688-100 100 µg 334.00 USD
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Product Specification

Immunogen:Synthetic peptide corresponding to the sequence near the C-terminus of quail Pax3.
UniProt ID:P23760
Source:Purified from hybridoma tissue culture supernatant.
Species reactivity:Human
Applications:IHC (PS), WB
Recommended Dilutions/Conditions:Western Blot (1µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~53kDa by Western blot.
Purity Detail:Protein G affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:Pax3 is a member of the paired box family of nuclear transcription factors that function in the transcriptional control of pattern development during embryogenesis. Pax3 is characterized by a conserved N-terminal paired box DNA binding domain, and a C-terminal transcriptional activation domain. In humans, loss of function mutations in Pax3 are associated with type I and type III Waardenburg Syndrome, characterized by varying degrees of developmental abnormalities in facial structure, pigmentation, limb development, and hearing. Translocation t(2;13) of the Pax3 loci to the fork head (FKHR) transcription factor gene results in the creation of a Pax3-FKHR fusion gene with transcriptional hyperactivity manifested in alveolar rhabdomyosarcoma.
Regulatory Status:RUO - Research Use Only
Pax3 monoclonal antibody (C2) Immunohistochemistry
IHC Analysis: Human skeletal muscle tissue stained with Pax3, mAb (C2) at 10µg/ml.
Pax3 monoclonal antibody (C2) Western blot
Western blot analysis of 624 Mel nuclear lysates probed with Pax-3 mAb (C2) at 1µg/ml
Pax3 monoclonal antibody (C2) Immunohistochemistry
IHC Analysis: Human skin tissue stained with Pax3, mAb (C2) at 10µg/ml.
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Pax3 monoclonal antibody (C2) Immunohistochemistry Pax3 monoclonal antibody (C2) Western blot Pax3 monoclonal antibody (C2) Immunohistochemistry

Product Literature References

Loading of PAX3 to Mitotic Chromosomes Is Mediated by Arginine Methylation and Associated with Waardenburg Syndrome: T.F. Wu, et al.; J. Biol. Chem. 290, 20556 (2015), Abstract; Full Text

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