| Alternative Name: | PDGFR, platelet-derived growth factor receptor |
| |
| Clone: | 42G12 |
| |
| Host: | Mouse |
| |
| Isotype: | IgG1 |
| |
| Immunogen: | Recombinant human PDGF Receptor β. |
| |
| UniProt ID: | P09619 |
| |
| Source: | Purified from mouse ascites. |
| |
| Species reactivity: | Mouse, Rat
|
| |
| Crossreactivity: | Weakly cross-reacts with human PDGF receptor β. |
| |
| Applications: | IHC (PS), WB
|
| |
| Recommended Dilutions/Conditions: | Western Blot (1µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
| |
| Application Notes: | Detects a band of ~125kDa by Western blot. |
| |
| Purity Detail: | Protein G affinity purified. |
| |
| Formulation: | Liquid. In PBS, pH 7.2, containing 0.09% sodium azide and 50% glycerol. |
| |
| Handling: | Avoid freeze/thaw cycles. |
| |
| Shipping: | Blue Ice |
| |
| Long Term Storage: | -20°C |
| |
| Scientific Background: | Mitogenic platelet derived growth factor (PDGF) signaling is mediated by the PDGF tyrosine kinase receptors PDGFRα and PDGFRβ, which exist as homo- or heterodimer isoforms (αα, ββ, αβ). Secretion of PDGFs and expression of PDGFRs in a broad distribution of cell types contributes to regulation of cellular processes including cell growth, actin reorganization, prevention of apoptosis, and chemotaxis. Structurally, the transmembrane PDGFRs are composed of an N-terminal extracellular domain with five immunoglobulin repeats, and an intracellular domain containing two spaced tyrosine kinase domains. PDGF ligands display preferential affinity to PDGFRα and PDGFRβ, with PDGFRβ activation restricted to binding by PDGF BB and PDGF DD. Growth factor binding initiates receptor dimerization and activates the cytosolic tyrosine kinase domains. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
IHC Analysis: Human spleen tissue stained with PDGF receptor β, mAb (42G12) at 20µg/ml.
Western blot analysis of MW marker (1) and mouse heart muscle lysate (2) probed with PDGF Receptor β mAb (42G12) (extra bands are secondary reactivity).
IHC Analysis: Human placenta tissue stained with PDGF receptor β, mAb (42G12) at 20µg/ml.
