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[pSer727]Stat3 monoclonal antibody (23G5)

ADI-905-672-100 100 µg 537.00 USD
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Product Details

Alternative Name:Signal transducer and activator of transcription 3
Immunogen:Synthetic peptide corresponding to a portion of Stat3 phosphorylated at Ser727.
UniProt ID:P40763
Species reactivity:Human, Mouse, Rat
Applications:ELISA, IHC, WB
Recommended Dilutions/Conditions:ELISA (0.1µg/ml)
Immunohistochemistry (1:50)
Western Blot (0.5µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~92kDa by Western blot.
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 3 months.
Shipping:Blue Ice
Long Term Storage:-20°C
Scientific Background:Stats (Signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, Stats enter the nucleus to regulate transcription of many different genes.
Regulatory Status:RUO - Research Use Only
[pSer727]Stat3 monoclonal antibody (23G5) Immunohistochemistry
Immunohistochemistry analysis of human lung cancer tissue with Stat3 (pSer727) mAb, clone 23G5.
[pSer727]Stat3 monoclonal antibody (23G5) Western blot
Western blot analysis of untreated (1), EGF stimulated (2), or pervanadate treated (3) A431 cell lysates with Stat3 (pSer727) mAb, clone 23G5.
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[pSer727]Stat3 monoclonal antibody (23G5) Immunohistochemistry [pSer727]Stat3 monoclonal antibody (23G5) Western blot

Product Literature References

Early methyl donor deficiency may induce persistent brain defects by reducing Stat3 signaling targeted by miR-124: R. Kerek, et al.; Cell Death Dis. 4, e755 (2013), Application(s): Immunofluorescence, Abstract; Full Text

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