| ADI-905-665-100 | 100 µg | 478.00 USD |  |
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| Alternative Name: | Src homology 2 domain containing |
| Clone: | 11F6 |
| Host: | Mouse |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide corresponding to the sequence near the C-terminus of Shc. |
| UniProt ID: | P29353 |
| Species reactivity: | Human, Mouse |
| Applications: | ELISA, ICC, IHC, WB |
| Recommended Dilutions/Conditions: | Immunohistochemistry (1:50) Western Blot (1µg/ml, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Application Notes: | Detects bands of ~46kDa, ~52kDa and ~66kDa by Western blot. |
| Purity Detail: | Thiophilic adsorption and size exclusion chromatography purified. |
| Formulation: | Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose. |
| Reconstitution: | Reconstitute with 1ml water (15 minutes at room temperature). |
| Use/Stability: | Stable at -80°C up to 1 year, at 4°C up to 3 months. |
| Shipping: | Shipped on Blue Ice |
| Long Term Storage: | -20°C |
| Scientific Background: | The Src homology 2 domain containing (Shc) adaptor protein links growth factor, cytokine, and antigen receptor stimulation to the Ras signaling pathway, and plays a critical role in insulin signaling, tumorigenesis, and hematopoietic cell growth and differentiation. Structurally, Shc is composed of an N-terminal phosphotyrosine binding (PTB) domain, a medial CH1 domain, and a C-terminal SH2 domain. Upon binding to activate cell-surface receptors, Shc is phosphorylated at three tyrosine residues (Tyr239, Tyr240, Tyr317) triggering recognition of Shc by SH2-containing proteins such as Grb2. |
| Regulatory Status: | RUO - Research Use Only |
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