Alternative Name: | Src homology 2 domain containing |
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Clone: | 11F6 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Synthetic peptide corresponding to the sequence near the C-terminus of Shc. |
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UniProt ID: | P29353 |
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Species reactivity: | Human, Mouse
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Applications: | ELISA, ICC, IHC, WB
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Recommended Dilutions/Conditions: | Immunohistochemistry (1:50) Western Blot (1µg/ml, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects bands of ~46kDa, ~52kDa and ~66kDa by Western blot. |
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Purity Detail: | Thiophilic adsorption and size exclusion chromatography purified. |
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Formulation: | Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose. |
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Reconstitution: | Reconstitute with 1ml water (15 minutes at room temperature). |
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Use/Stability: | Stable at -80°C up to 1 year, at 4°C up to 3 months. |
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Shipping: | Shipped on Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | The Src homology 2 domain containing (Shc) adaptor protein links growth factor, cytokine, and antigen receptor stimulation to the Ras signaling pathway, and plays a critical role in insulin signaling, tumorigenesis, and hematopoietic cell growth and differentiation. Structurally, Shc is composed of an N-terminal phosphotyrosine binding (PTB) domain, a medial CH1 domain, and a C-terminal SH2 domain. Upon binding to activate cell-surface receptors, Shc is phosphorylated at three tyrosine residues (Tyr239, Tyr240, Tyr317) triggering recognition of Shc by SH2-containing proteins such as Grb2. |
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Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of cancer cell lysates with Shc mAb (11F6); HeLa (1), HepG2 (2), HEK293 (3), SH-SY5Y (4), MDCK (5), PC12 (6), CMT93 (7), Neuro 2A (8), NIH-3T3 (9).
Immunofluorescent analysis (confocal) staining of V79 cells using Shc mAb (11F6) (green); nuclei are stained in blue pseudocolor using DRAQ5.
Immunohistochemistry analysis of human hepatoma tissue with Shc mAb (11F6).