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[pSer218/222]MEK1 and [pSer222/226]MEK2 monoclonal antibody (7E10)

ADI-905-652-100 100 µg 490.00 USD
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Product Details

Alternative Name:MKK1/MKK2, MAP kinase kinase 1/2
Immunogen:Synthetic peptide corresponding to a portion of MEK1 phosphorylated at Ser218/222and MEK2 phosphorylated at Ser222/226 , conjugated to KLH.
UniProt ID:Q02750 (MEK1), P36507 (MEK2)
Species reactivity:Human, Mouse
Applications:ELISA, WB
Recommended Dilutions/Conditions:ELISA (0.1µg/ml)
Western Blot (0.5µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~45kDa by Western blot.
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 3 months.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:MEK is a dual specificity kinase capable of phosphorylating both tyrosine and threonine residues. The MEK family, which is also known as MAP kinase kinase, phosphorylates MAP kinases on the conserved T-X-Y motif; phosphorylation of MAPK by MEK results in an increase in MAPK activity. MEK is involved in a diverse array of cellular processes such as stress-activated response, apoptosis, cytokine-induced cell proliferation, and DNA recombination during meiosis.
Regulatory Status:RUO - Research Use Only
[pSer218/222]MEK1 and [pSer222/226]MEK2 monoclonal antibody (7E10) Western blot
Western blot analysis of untreated control (co), EGF stimulated (EGF), or pervanadate treated (VH) A549 cell lysates, probed with [pS218/222]MEK1 [pS222/226]MEK2, mAb (7E10) (0.5 μg/ml).
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[pSer218/222]MEK1 and [pSer222/226]MEK2 monoclonal antibody (7E10) Western blot

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