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[pSer^218/222]MEK1 and [pSer^222/226]MEK2 monoclonal antibody (7E10)



ADI-905-652-100	100 µg	551.00 USD
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Product Details

Alternative Name:	MKK1/MKK2, MAP kinase kinase 1/2

Clone:	7E10

Host:	Mouse

Isotype:	IgG1

Immunogen:	Synthetic peptide corresponding to a portion of MEK1 phosphorylated at Ser^218/222and MEK2 phosphorylated at Ser^222/226 , conjugated to KLH.

UniProt ID:	Q02750 (MEK1), P36507 (MEK2)

Species reactivity:	Human, Mouse Dog

Applications:	ELISA, WB

Recommended Dilutions/Conditions:	ELISA (0.1µg/ml) Western Blot (0.5µg/ml, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Application Notes:	Detects a band of ~45kDa by Western blot.

Purity Detail:	Thiophilic adsorption and size exclusion chromatography purified.

Formulation:	Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.

Reconstitution:	Reconstitute with 1ml water (15 minutes at room temperature).

Use/Stability:	Stable at -80°C up to 1 year, at 4°C up to 3 months.

Shipping:	Blue Ice

Long Term Storage:	-20°C

Scientific Background:	MEK is a dual specificity kinase capable of phosphorylating both tyrosine and threonine residues. The MEK family, which is also known as MAP kinase kinase, phosphorylates MAP kinases on the conserved T-X-Y motif; phosphorylation of MAPK by MEK results in an increase in MAPK activity. MEK is involved in a diverse array of cellular processes such as stress-activated response, apoptosis, cytokine-induced cell proliferation, and DNA recombination during meiosis.

Regulatory Status:	RUO - Research Use Only

[pSer218/222]MEK1 and [pSer222/226]MEK2 monoclonal antibody (7E10) Western blot

Western blot analysis of untreated control (co), EGF stimulated (EGF), or pervanadate treated (VH) A549 cell lysates, probed with [pS218/222]MEK1 [pS222/226]MEK2, mAb (7E10) (0.5 μg/ml).

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