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Jnk1 monoclonal antibody (5D10)

ADI-905-649-100 100 µg 529.00 USD
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Product Details

Alternative Name:c-Jun N-terminal kinase 1, SAP kinase 1c, SAPK1c, MAP kinase 8, MAPK8
Immunogen:Synthetic peptide corresponding to the sequence near the N-terminus of Jnk1.
UniProt ID:P45983
Species reactivity:Human, Mouse, Rat
Applications:ELISA, IHC, WB
Recommended Dilutions/Conditions:ELISA (0.05µg/ml)
Immunohistochemistry (1:50)
Western Blot (0.5µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~45kDa by Western blot.
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 3 months.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:The Stress-activated protein kinases alpha and gamma (SAPKalpha and gamma), also know as c-Jun NH2-terminal kinases 1 and 2 (Jnk1/2) are serine/ threonine protein kinases belonging to the MAP kinase family. The extracellular stimuli leading to cell stress include inflammatory cytokines (via Rho and Rac), growth factors, protein synthesis inhibitors and osmotic stress amongst others. Both Jnk1 and Jnk2 must be dually phosphorylated in the activation loop, at threonine 183 and tyrosine 185, to achieve full enzymatic activity.
Regulatory Status:RUO - Research Use Only
Jnk1 monoclonal antibody (5D10) Western blot
Western blot analysis of serum starved cancer cells A431 (1), A549 (2), SKOV3 (3), OVCAR5 (4), HaCat (5), PC3 (6), HeLa (7) and HepG2 (8), probed with Jnk1 mAb (5D10).
Jnk1 monoclonal antibody (5D10) Immunohistochemistry
Immunohistochemistry analysis of human hepatoma tissue with Jnk1 mAb (5D10).
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Jnk1 monoclonal antibody (5D10) Western blot Jnk1 monoclonal antibody (5D10) Immunohistochemistry

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