Alternative Name: | INSR |
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Clone: | 9H4 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Synthetic peptide corresponding to a portion of insulin receptor. |
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UniProt ID: | P06213 |
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Species reactivity: | Human, Mouse, Rat Dog
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Applications: | ELISA, IHC, WB
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Recommended Dilutions/Conditions: | ELISA (0.1µg/ml) Immunohistochemistry (1:50) Western Blot (0.1µg/ml) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects a band of ~97kDa by Western blot. |
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Purity Detail: | Thiophilic adsorption and size exclusion chromatography purified. |
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Formulation: | Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose. |
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Reconstitution: | Reconstitute with 1ml water (15 minutes at room temperature). |
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Use/Stability: | Stable at -80°C up to 1 year, at 4°C up to 3 months. |
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Shipping: | Shipped on Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | The Insulin Receptor (InsR) is a heterodimeric receptor tyrosine kinase with an extracellular alpha-chain, a transmembrane domain and an intracellular beta-chain. InsR is activated upon binding of the peptide hormone insulin, leading to autophosphorylation of tyrosine residues 1146, 1150, and 1151 in the activation loop of the beta-chain. Additional autophosphorylation sites such as tyrosine residues 960, 972, 1316, and 1322 regulate the assembly of signal transduction complexes. |
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Regulatory Status: | RUO - Research Use Only |
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Immunohistochemistry analysis of human hepatoma tissue with Insulin Receptor mAb (9H4).
Western blot analysis of serum starved cancer cells A431 (1), SW480 (2), SW620 (3), HT29 (4), MCF -7 (5), MDA-MB231 (6), and T47D (7), probed with Insulin Receptor mAb (9H4).