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[pTyr1322]Insulin receptor monoclonal antibody (21G12)

 
ADI-905-646-100 100 µg 454.00 USD
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Product Specification

Alternative Name:INSR
 
Clone:21G12
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Synthetic peptide corresponding to a portion of insulin receptor phosphorylated at Tyr1322.
 
UniProt ID:P06213
 
Species reactivity:Human, Mouse
Dog
 
Applications:ELISA, WB
 
Recommended Dilutions/Conditions:ELISA (0.1µg/ml)
Western Blot (0.5µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~97kDa by Western blot.
 
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
 
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
 
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
 
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 3 months.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The Insulin Receptor (InsR) is a heterodimeric receptor tyrosine kinase with an extracellular alpha-chain, a transmembrane domain and an intracellular beta-chain. InsR is activated upon binding of the peptide hormone insulin, leading to autophosphorylation of tyrosine residues 1146, 1150, and 1151 in the activation loop of the beta-chain. Additional autophosphorylation sites such as tyrosine residues 960, 972, 1316, and 1322 regulate the assembly of signal transduction complexes.
 
Regulatory Status:RUO - Research Use Only
 
[pTyr1322]Insulin receptor monoclonal antibody (21G12) Western blot
Western blot analysis of MDA-MB-231 cell lysates, untreated (1), Insulin treated (2), IGF-1 treated (3), or pervanadate treated (4), probed with Insulin Receptor (pTyr1322) mAb (21G12).
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[pTyr1322]Insulin receptor monoclonal antibody (21G12) Western blot

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