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Fos monoclonal antibody (8B5)

ADI-905-641-100 100 µg 469.00 USD
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Product Details

Immunogen:Synthetic peptide corresponding to a portion of human c-Fos .
UniProt ID:P01100
Species reactivity:Human, Mouse, Rat
Applications:ELISA, IHC, WB
Recommended Dilutions/Conditions:ELISA (0.1µg/ml)
Immunohistochemistry (1:50)
Western Blot (0.5µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~50kDa by Western blot.
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 3 months.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:The immediate early gene product c-Fos is expressed following mitogenic stimulation and functions as a sensor for MAPK signal duration. When MAPK activation is sustained, c-Fos is phosphorylated by MAPK at Ser374. Phosphorylation stabilizes the c-Fos protein and primes c-Fos for additional phosphorylation at Thr325. During transient MAPK activation, activity declines before accumulation of the c-Fos protein.
Regulatory Status:RUO - Research Use Only
Fos monoclonal antibody (8B5) Immunohistochemistry
Immunohistochemistry analysis of human hepatoma tissue with c-Fos mAb (8B5).
Fos monoclonal antibody (8B5) Western blot
Western blot analysis of serum -starved cancer cells A431 (1), A549 (2), SKOV3 (3), OVCAR5 (4), HaCaT (5), PC3 (6), HeLa (7) and HepG2 (8), probed with c-Fos mAb (8B5).
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Fos monoclonal antibody (8B5) Immunohistochemistry Fos monoclonal antibody (8B5) Western blot

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