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[pSer³⁷⁴]Fos monoclonal antibody (34E4)



ADI-905-640-100	100 µg	522.00 USD
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Product Details

Clone:	34E4

Host:	Mouse

Isotype:	IgG1

Immunogen:	Synthetic peptide corresponding to a portion of human c-Fos phosphorylated at Ser³⁷⁴.

UniProt ID:	P01100

Species reactivity:	Human Dog

Applications:	ELISA, IHC, WB

Recommended Dilutions/Conditions:	ELISA (0.1µg/ml) Immunohistochemistry (1:50) Western Blot (0.5µg/ml, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Application Notes:	Detects a band of ~50kDa by Western blot.

Purity Detail:	Thiophilic adsorption and size exclusion chromatography purified.

Formulation:	Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.

Reconstitution:	Reconstitute with 1ml water (15 minutes at room temperature).

Use/Stability:	Stable at -80°C up to 1 year, at 4°C up to 3 months.

Shipping:	Blue Ice

Long Term Storage:	-20°C

Scientific Background:	The immediate early gene product c-Fos is expressed following mitogenic stimulation and functions as a sensor for MAPK signal duration. When MAPK activation is sustained, c-Fos is phosphorylated by MAPK at Ser374. Phosphorylation stabilizes the c-Fos protein and primes c-Fos for additional phosphorylation at Thr325. During transient MAPK activation, activity declines before accumulation of the c-Fos protein.

Regulatory Status:	RUO - Research Use Only

[pSer374]Fos monoclonal antibody (34E4) Western blot

Western blot analysis of control (1) and serum -starved cancer cells treated with 10ng/ml EGF for 5 min (2), 15 min (3), 30 min (4), 1 hr (5), 2 hrs (6), 4 hrs (7) and 8 hrs (9), probed with c -Fos (pSer374) mAb (34E4).