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[pSer¹³³]CREB monoclonal antibody (10E9)



ADI-905-639-100	100 µg	510.00 USD
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Product Details

Alternative Name:	cAMP responsive element binding protein

Clone:	10E9

Host:	Mouse

Isotype:	IgG1

Immunogen:	Synthetic peptide corresponding to a portion of CREB phosphorylated at Ser¹³³.

UniProt ID:	P16220 (human)

Species reactivity:	Human, Mouse Dog

Applications:	ELISA, WB

Recommended Dilutions/Conditions:	ELISA (0.05µg/ml) Western Blot (0.5µg/ml, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Application Notes:	Detects bands of ~43kDa (CREB) and ~38kDa (ATF1) by Western blot.

Purity Detail:	Thiophilic adsorption and size exclusion chromatography purified.

Formulation:	Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.

Reconstitution:	Reconstitute with 1ml water (15 minutes at room temperature).

Use/Stability:	Stable at -80°C up to 1 year, at 4°C up to 3 months.

Shipping:	Blue Ice

Long Term Storage:	-20°C

Scientific Background:	The transcription factor CREB (cAMP response element binding) binds the palindromic octanucleotide TGACGTCA. Members of the ATF/CREB family include CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2, and ATF-3. Phosphorylation of CREB allows recruitment of CREB-binding protein (CBP), a large co-activator that contacts the general transcriptional machinery. CREB-mediated transcription regulates diverse cellular responses, including intermediary metabolism, neuronal signaling, cell proliferation, and apoptosis.

Regulatory Status:	RUO - Research Use Only

[pSer133]CREB monoclonal antibody (10E9) Western blot

Western blot analysis of untreated (1), EGF-stimulated (2), or pervanadate-treated (3) A549 cancer cell lysates probed with CREB (pSer133) mAb (10E9).

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