Alternative Name: | cAMP responsive element binding protein |
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Clone: | 10E9 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Synthetic peptide corresponding to a portion of CREB phosphorylated at Ser133. |
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UniProt ID: | P16220 (human) |
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Species reactivity: | Human, Mouse Dog
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Applications: | ELISA, WB
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Recommended Dilutions/Conditions: | ELISA (0.05µg/ml) Western Blot (0.5µg/ml, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects bands of ~43kDa (CREB) and ~38kDa (ATF1) by Western blot. |
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Purity Detail: | Thiophilic adsorption and size exclusion chromatography purified. |
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Formulation: | Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose. |
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Reconstitution: | Reconstitute with 1ml water (15 minutes at room temperature). |
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Use/Stability: | Stable at -80°C up to 1 year, at 4°C up to 3 months. |
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Shipping: | Shipped on Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | The transcription factor CREB (cAMP response element binding) binds the palindromic octanucleotide TGACGTCA. Members of the ATF/CREB family include CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2, and ATF-3. Phosphorylation of CREB allows recruitment of CREB-binding protein (CBP), a large co-activator that contacts the general transcriptional machinery. CREB-mediated transcription regulates diverse cellular responses, including intermediary metabolism, neuronal signaling, cell proliferation, and apoptosis. |
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Regulatory Status: | RUO - Research Use Only |
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