| Alternative Name: | OPN, SPP1, Secreted phosphoprotein 1, Bone sialoprotein 1 |
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| Clone: | 87-B |
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| Host: | Mouse |
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| Isotype: | IgG2a |
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| Immunogen: | Recombinant GST tagged human osteopontin. |
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| UniProt ID: | P10451 |
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| Source: | Purified from mouse ascites. |
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| Species reactivity: | Human
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| Specificity: | Recognizes full length OPN and C-terminal portion of thrombin-cleaved OPN. |
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| Crossreactivity: | Does not cross-react with mouse or bovine OPN. |
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| Applications: | ELISA, IHC (FS), IHC (PS), WB
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| Recommended Dilutions/Conditions: | Immunohistochemistry: staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate, pH 6.0, for 10min followed by cooling at RT for 20min.
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Osteopontin (OPN) is an acidic extracellular matrix cell adhesion protein that is relatively abundant not only in bone matrix, plasma, urine, and milk, but is also found in malignant and atherogenic tissues. Phosphorylation, glycosylation and calcium modifications allow intact and fragmented Osteopontin to direct a variety of diverse responses including tissue remodeling, inflammation and cell survival. Plasma Osteopontin has been shown to provide prognostic information in breast, prostate, colon, and lung cancers as well as metastatic carcinomas. The notable presence of Osteopontin in a variety of tumors is strongly correlated to pathological stage, suggesting its critical role in tumor invasiveness, progression and metastasis. In addition, Osteopontin inhibits inducible nitric oxide synthase activity, thereby protecting tumor cells from NO-mediated macrophage cytotoxic attack. Osteopontin is found in atherosclerotic plaques and may drive a number of diabetic vascular pathologies. |
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| Technical Info/Product Notes: | For immunohistochemistry, staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate, pH6.0 for 10min followed by cooling at RT for 20min. Recognizes full length human OPN and C-terminal portion of thrombin-cleaved human OPN. Does not react with mouse or bovine OPN. |
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| Regulatory Status: | RUO - Research Use Only |
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