| Alternative Name: | APIP |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to the sequence near the C-terminus of human Apaf1. The sequence differs from that of mouse Apaf1 by one amino acid. |
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| UniProt ID: | O14727 |
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| Species reactivity: | Human, Mouse, Rat
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| Applications: | IHC, WB
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| Recommended Dilutions/Conditions: | Western Blot (1:500-1:2,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~130kDa by Western blot. |
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| Purity Detail: | Peptide affinity purified. |
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| Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Apaf-1 (Apoptosis protease-activating factor 1), a mammalian protein homologous to C. elegans CED-4, is an essential adaptor molecule and a key linker of the mitochondrial apoptotic pathway. Apaf-1 binds to Cytochrome c (Apaf-2) leading to the formation of a large multimeric complex. This complex recruits (through CARD) Procaspase-9 and once activated, caspase-9 (Apaf-3) dissociates. Activated caspase-9 in turn cleaves and activates caspase-3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf-1 can also associate with caspase-4, caspase-8 and Diva. Apaf-1 is ubiquitously expressed in human tissues. |
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| Regulatory Status: | RUO - Research Use Only |
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