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AIF (IN) polyclonal antibody

ADI-905-168-100 100 µg 467.00 USD
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Product Details

Alternative Name:Apoptosis-inducing factor
Immunogen:Synthetic peptide corresponding to a portion of human AIF. The sequence is conserved in mouse and rat AIF.
UniProt ID:O95831
Species reactivity:Human, Mouse, Rat
Applications:IHC, WB
Recommended Dilutions/Conditions:Immunohistochemistry (10µg/ml)
Western Blot (1.0-2.0µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~67kDa by Western blot.
Purity Detail:Ion exchange purified.
Formulation:Liquid. In PBS containing 0.02% sodium azide.
Shipping:Blue Ice Not Frozen
Long Term Storage:+4°C
Scientific Background:Apoptosis inducing factor (AIF) is a mitochondrial inter-membrane flavoprotein that is ubiquitously expressed both in normal tissues and in a variety of cancer cell lines. AIF is synthesized as a nonapoptogenic precursor in the cytoplasm and efficiently imported into the mitochondrial inter-membrane space. Mature AIF protein is normally confined to the mitochondrial inter-membrane space. In a variety of different apoptosis-inducing conditions AIF translocates through the outer mitochondrial membrane to the cytosol and to the nucleus. Extra-mitochondrial AIF induces nuclear chromatin condensation and large scale (~50kb) DNA fragmentation.
Regulatory Status:RUO - Research Use Only
AIF (IN) polyclonal antibody Immunohistochemistry
Immunohistochemistry analysis of AIF in human retina with AIF antibody at 10µg/ml.
AIF (IN) polyclonal antibody Western blot
Western blot analysis of AIF in K562 cell lysate (A), rat heart (B), and mouse heart (C) tissue lysates with AIF antibody (IN) at 1µg/ml.
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AIF (IN) polyclonal antibody Immunohistochemistry AIF (IN) polyclonal antibody Western blot

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