Product Details
| Host: | Rabbit |
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| Immunogen: | 12(S)-HETE conjugated to human serum albumin. |
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| Source: | Whole rabbit serum. |
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| Species reactivity: | Species independent
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| Applications: | ELISA
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| Formulation: | Liquid. Contains sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | HETEs are byproducts generated by the metabolism of arachidonic acid by lipoxygenases. 12(S)-HETE is the stereospecific hydroxy product from the reduction of 12(S)-hydroperoxy tetraenoic eicosatetraenoic acid [12(S)-HpETE], which itself is a 12-lipoxygenase metabolite of arachidonic acid. 12(S)-HETE is chemotactic and chemokinetic for polymorphonuclear leukocytes and vascular smooth muscle cells. It also acts as a second messenger in angiotensin-II induced aldosterone production. 15-hydroxyeicosatetraenoic acid [15(S)-HETE] is the major hydroxy derivative of arachidonic acid when acted upon by 15-lipoxygenase. It is also the primary monohydroxy acid synthesized by the lipoxygenase activity of Cyclooxygenase-1 upon linoleic acid. 15(S)-HETE acts as a paracrine regulator of smooth muscle and lung neutrophil recruitment, and elevated levels of 15(S)-HETE are associated with asthma, rhinitis, chronic paranasal sinusitis and rheumatoid arthritis. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Distinct roles of N-acetyl and 5-methoxy groups in the antiproliferative and neuroprotective effects of melatonin: R. Letra-Vilela, et al.; Mol. Cell Endocrinol.
434, 238 (2016),
Application(s): Cell culture,
Abstract;
Estradiol-17β increases 12- and 15-lipoxygenase (type2) expression and activity and reactive oxygen species in human umbilical vascular smooth muscle cells: D. Somjen, et al.; J. Steroid Biochem. Mol. Biol.
163, 28 (2016),
Abstract;
Neutrophils rapidly transit inflamed lymphatic vessel endothelium via integrin-dependent proteolysis and lipoxin-induced junctional retraction: D.A. Rigby, et al.; J. Leukoc. Biol.
98, 897 (2015),
Abstract;
Full Text
General Literature References
Oral green tea catechin metabolites are incorporated into human skin and protect against UV radiation-induced cutaneous inflammation in association with reduced production of pro-inflammatory eicosanoid 12-hydroxyeicosatetraenoic acid: L.E. Rhodes, et al.; Br. J. Nutr.
28, 1 (2013),
Abstract;
