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Highly sensitive ELISA, measuring as liitle as 8.4 pg/ml
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High throughput format with results for up to 39 samples in duplicate in just 4 hours
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Stable liquid color coded reagents and pre-coated plates reduce errors
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Fully quantitative results that surpass semi-quantitative Western blot analysis
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Save with bulk kit package!
The TNF-α (human), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 4 hours. Absorbance is read at 450 nm. Assay up to 39 samples in duplicate per 96-well plate. The broad dynamic range makes assaying different pathologies easy. The larger package size offers a lower cost-per-test.
Product Details
| Alternative Name: | Tumor necrosis factor-α, TNFSF 2 |
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| Sensitivity: | 8.43 pg/ml (range 15.63 - 1,000 pg/ml) |
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| Assay Time: | 4 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of human TNF-α in culture supernatants, plasma, and serum. |
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| Wavelength: | 450 nm |
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| Species reactivity: | Human
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| Use/Stability: | Store all components at +4°C, except standard at -20°C. |
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| Shipping: | Blue Ice |
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| Contents: | Microtiter plate, Conjugate, Antibody, Assay buffer 13 concentrate, Wash buffer concentrate, Standard, TMB Substrate, Stop solution 2 |
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| Scientific Background: | Tumor Necrosis Factor-α (TNF-α) is a 17.5 kDa, 157 amino acid protein that is a potent lymphoid factor, which exerts cytotoxic effects on a wide range of tumor cells and other target cells. TNF-α has been suggested to play a pro-inflammatory role and has been detected in synovial fluid of patients with rheumatoid arthritis. It is the primary mediator of immune regulation. The biosynthesis of TNF-α is tightly controlled, being produced in extremely small quantities in quiescent cells, but is a major secreted factor in activated cells. |
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| Technical Info/Product Notes: | Cited samples:
Cytokine ELISAs Cited Samples |
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| UniProt ID: | P01375 |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Anti-inflammatory properties of S53P4 bioactive glass implant material: F.N. Barrak, et al.; J. Dent.
127, 104296 (2022),
Abstract;
Exosomal circSHKBP1 participates in non-small cell lung cancer progression through PKM2-mediated glycolysis: W. Chen, et al.; Mol. Ther. Oncolytics
24, 470 (2022),
Abstract;
BACH1 is transcriptionally inhibited by TET1 in hepatocellular carcinoma in a microRNA-34a-dependent manner to regulate autophagy and inflammation: X. Sun, et al.; Pharmacol. Res.
169, 105611 (2021),
Abstract;
Progranulin concentration in relation to bone mineral density among obese individuals: A. Milajerdi, et al.; Arch. Endocrinol. Metab.
62, 179 (2018),
Application(s): EIA w/ human serum ,
Abstract;
Full Text
Effects of Erythrocyte Membrane Polyunsaturated Fatty Acids in Overweight, Obese, and Morbidly Obese Korean Women: H.N. Choi, et al.; J. Cancer Prev.
22, 182 (2017),
Application(s): Human serum,
Abstract;
Full Text
Evaluation of HIV-DNA and inflammatory markers in HIV-infected individuals with different viral load patterns: F. Falasca, et al.; BMC Infect. Dis.
17, 581 (2017),
Abstract;
Full Text
Serum adipocyte-fatty acid binding protein (FABP4) levels in women from Mexico exposed to polycyclic aromatic hydrocarbons (PAHs): Á.C. Ochoa-Martínez, et al.; Environ. Sci. Pollut. Res. Int.
24, 1862 (2017),
Application(s): ELISA of human serum,
Abstract;
Activating transcription factor-3 induction is involved in the anti-inflammatory action of berberine in RAW264.7 murine macrophages: Y.A. Bae, et al.; Korean J. Physiol. Pharmacol.
20, 415 (2016),
Application(s): ELISA (to find proinflammatory cytokine production),
Abstract;
Full Text
The Effect of Onion Peel Extract on Inflammatory Mediators in Korean Overweight and Obese Women: K.A. Kim & J.E. Yim; Clin. Nutr. Res.
5, 261 (2016),
Application(s): Plasma samples,
Abstract;
Full Text
Vitamin D2 suppresses amyloid-β 25-35 induced microglial activation in BV2 cells by blocking the NF-κB inflammatory signaling pathway: S. Raha, et al.; Life Sci.
161, 37 (2016),
Application(s): Assessment of the inhibitory effect of VD2 on the production of pro-inflammatory cytokines,
Abstract;
Ginseng marc-derived low-molecular weight oligosaccharide inhibits the growth of skin melanoma cells via activation of RAW264.7 cells: J.Y. Seo, et al.; Int. Immunopharmacol.
29, 344 (2015),
Application(s): Quantitation of cytokine levels in cultural supernates,
Abstract;
Association between obesity-related adipokines and colorectal cancer: A case-control study and meta-analysis: R.K. Joshi, et al.; World J. Gastroenterol.
20, 7941 (2014),
Application(s): Quantification of TNF-α in serum samples,
Abstract;
Full Text
Genetic damage in patients with chronic kidney disease, peritoneal dialysis and haemodialysis: a comparative study: A. Rangel-Lopez, et al.; Mutagenesis
28, 219 (2013),
Application(s): ELISA using human serum,
Abstract;
Full Text
Effects of red orange juice intake on endothelial function and inflammatory markers in adult subjects with increased cardiovascular risk: S. Buscemi, et al.; Am. J. Clin. Nutr.
95, 1089 (2012),
Application(s): Levels of IL-6 in human serum,
Abstract;
Full Text
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