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11-dehydro-TXB2 ELISA kit

 
ADI-901-092 5x96 wells 1,686.00 USD
Do you need bulk/larger quantities?
 
Alternative size available: ADI-900-092 (1x96 wells)
  • Higher throughput - measure up to 38 samples in duplicate in just 3 hours
  • Easy-to-use - works with any standard colorimetric plate reader, no specialized training required
  • Ready-to-use liquid color-coded reagents save time and reduce errors
  • More sensitive than other commercially available kits
  • Specific - low reactivity to structurally related molecules
  • Save with bulk kit package!
The 11-dehydro-TXB2 EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 3 hours. Absorbance is read at 405 nm. This kit offers ultrasensitive quantification of 11-d-TXB2 in a variety of sample matrices with negligible reactivity with related molecules in a convenient 96-well format offers higher throughput analysis compared to conventional analytical methods.
11-dehydro-TXB2 ELISA kit Standard curve
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11-dehydro-TXB2 ELISA kit Standard curve

Product Details

Alternative Name:11-dehydro-Thromboxane B2
 
Sensitivity:4.31 pg/ml (range 9.8 - 10,000 pg/ml)
 
Assay Time:3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of 11-dehydro-TXB2 in culture supernatants and urine from any species. Cited samples include serum.
 
Wavelength:405 nm
 
Species reactivity:Species independent
 
Use/Stability:Store all components at +4°C, except standard and conjugate at -20°C.
 
Shipping:Shipped on Blue Ice
 
Contents:GxR Microtiter plate, Conjugate, Antibody, Assay buffer concentrate, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
 
Scientific Background:Thromboxane A2 (TXA2) is involved in platelet aggregation, vasoconstriction and reproductive functions, but has a half life of only 37 seconds under physiological conditions. Thromboxane B2 (TXB2) is the stable product of the non-enzymatic hydration of TXA2, thus the production of TXA2 in vivo is typically monitored by measurement of TXB2 and its metabolite 2,3-dinor TXB2. 11-dehydro-TXB2 is a major metabolite of Thromboxane B2 (TXB2) found in urine and plasma produced by its dehydrogenation by the enzyme 11-OH-dehydrogenase.
 
Regulatory Status:RUO - Research Use Only
 
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

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Product Literature References

Infection of Galleria mellonella (lepidoptera) larvae with the entomopathogenic fungus Conidiobolus coronatus (entomophthorales) induces apoptosis of hemocytes and affects the concentration of eicosanoids in the hemolymph: A.K. Wronska, et al.; Front. Physiol. 12, 774086 (2022), Application(s): ELISA kit using hemolymph, Abstract; Full Text
Effect of aspirin treatment on abacavir-associated platelet hyperreactivity in HIV-infected patients: E. Falcinelli, et al.; Int. J. Cardiol. 263, 118 (2018), Application(s): Human Urine, Abstract;
Moderate intake of docosahexaenoic acid raises plasma and platelet vitamin E levels in cystic fibrosis patients: E. Vericel, et al.; Prostaglandins Leukot. Essent. Fatty Acids 115, 41 (2016), Application(s): Basal formation of platelet thromboxane B2 and its urinary 11-dehydro metabolite, Abstract; Full Text
Moderate oral supplementation with docosahexaenoic acid improves platelet function and oxidative stress in type 2 diabetic patients: E. Véricel, et al.; Thromb. Haemost. 114, 289 (2015), Application(s): Platelet isolates from human blood samples, Abstract;
Perioperative management of aspirin resistance after off‐pump coronary artery bypass grafting: possible role for aprotinin: R.S. Poston, et al.; Transfusion 48, 39S (2008), Application(s): EIA using a variety of human serum, Abstract; Full Text
Simultaneous “hybrid” percutaneous coronary intervention and minimally invasive surgical bypass grafting: feasibility, safety, and clinical outcomes: B. Reicher, et al.; Am. Heart J. 155, 661 (2008), Application(s): EIA using a variety of human serum, Abstract; Full Text
The effects of COX-2 selective and non-selective NSAIDs on the initiation and progression of atherosclerosis in ApoE-/- mice: J. Metzner, et al.; J. Mol. Med. 85, 623 (2007), Application(s): EIA using a variety of human urine, Abstract; Full Text
Off-pump coronary artery bypass leads to a regional hypercoagulable state not detectable using systemic markers: Z.N. Kon, et al.; Innovations (Phila) 1, 232 (2006), Application(s): EIA using a variety of human serum, Abstract; Full Text
Increased susceptibility to pulmonary hypertension in heterozygous BMPR2-mutant mice: Y. Song, et al.; Circulation 112, 553 (2005), Application(s): EIA using a variety of human urine, Abstract;

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