- Quantitate PGI2 - measuring the stable breakdown products 6-keto-PGF1α and 2,3-dinor-6keto-PGF1α in one simple assay
- Less stress - much easier than GC/MS with results from up to 39 samples in duplicate in under 3 hours
- Specific - low cross-reactivity with related eicosanoids
- Save with bulk kit package!
The Urinary Prostacyclin EIA kit is a colorimetric competitive enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 405 nm. Quantitate PGI2 by measuring the stable metabolites in one simple assay.
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Product Details
| Alternative Name: | PGI2, Prostaglandin I2 |
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| Sensitivity: | 6.58 pg/ml (range 7.81 - 2,000 pg/ml) |
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| Assay Time: | <3 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of urinary prostacyclin metabolites in culture supernatants and urine from any species. |
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| Wavelength: | 405 nm |
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| Species reactivity: | Species independent
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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| Contents: | DxS Microtiter plate, Conjugate, Antibody, Assay buffer concentrate, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution |
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| Scientific Background: | Prostacyclin (PGI2) is involved in platelet aggregation, vasoconstriction, and reproductive functions. PGI2 has a half life of 60 minutes in plasma but only 2 to 3 minutes in buffer. The production of PGI2 is typically monitored by measurement of 6-keto-PGF1α and 2,3-dinor-6-keto-PGF1α. 6-keto-PGF1α is produced by the non-enzymatic hydration of PGI2, and is further metabolized in urine to the 2,3-dinor derivative. A number of pharmaceuticals alter and/or inhibit the synthesis of PGI2. Methods to measure PGI2 in urine typically involve HPLC, gas chromatography/mass spectrometry, or radioimmunoassay and enzyme immunoassay for 6-keto-PGF1α. The conversion of PGI2 into 6-keto-PGF1α and 2,3-dinor-6-keto-PGF1α is shown below. |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Hypothyroidism Affects Uterine Function via the Modulation of Prostaglandin Signaling: I. Kowalczyk-Zięba, et al.; Animals (Basel)
11, 2636 (2021),
Application(s): Rat urine,
Abstract;
Full Text
Identifying diagnostic endocrine markers and changes in endometrial gene expressions during pyometra in cats: E. Jursza-Piotrowska & M.J. Siemieniuch; Reprod. Biol.
16, 174 (2016),
Application(s): Concentrations of prostacyclin (PGI2) in plasma,
Abstract;
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