Replaces Prod. #: ADI-901-018
- Highly sensitive assay, measuring as little as 5.3 pg/ml of Substance P
- Widely cited in numerous peer reviewed publications
- High throughput fomat, assay up to 38 samples in duplicate per 96 well kit
- Fully quantitative results that surpass semi-quantitative Western blot analysis
- Save with bulk kit package!
The Substance P EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 3 hours. Absorbance is read at 405 nm. This kit utilized low sample volume and provides the ability to assay up to 38 samples in duplicate per 96 well kit.
Product Details
| Sensitivity: | 5.3 pg/ml (range 9.76 - 10,000 pg/ml) |
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| Assay Time: | 3 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of Substance P in culture supernatants, plasma, serum, saliva, and urine from any species. Cited sample types include tissue. |
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| Wavelength: | 405 nm |
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| Species reactivity: | Species independent
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| Use/Stability: | Store components at temperatures indicated on the component labeling. The Antibody, Conjugate and Standard are stored at -20°C and the remaining components are stored at 4°C. For convenience upon receipt, the unopened kit containing all components can be stored at -20°C and are stable at that temperature until the kit’s expiration date. After first thaw, store components at temperature indicated on the labeling as indicated above. Freeze-thaw cycles should be limited for optimal kit performance. Please see Reagent Preparation section in the product manual for more detailed component handling information. |
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| Shipping: | Shipped on Blue Ice |
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| Contents: | GxR Microtiter plate, Conjugate, Antibody, Assay buffer concentrate, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution |
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| Scientific Background: | Substance P is an undecapeptide hormone (RPKPQQFFGLM) that mediates a number of biological activities including nausea, anxiety, pain, and vasodilation. It is a member of the tachykinin family of neuropeptides, and is widely distributed in the peripheral and central nervous systems of vertebrates. |
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| UniProt ID: | P20366 |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Elevated serum substance P during simian varicella virus infection in rhesus macaques: implications for chronic inflammation and adverse cerebrovascular events: A. Bubak, et al.; J. Neurovirol.
26, 945 (2020),
Abstract;
Role of the afferent renal nerves in sodium homeostasis and blood pressure regulation in rats: A.A. Frame, et al.; Exp. Physiol.
104, 1306 (2019),
Application(s): ELISA using culture supernatants,
Abstract;
Sensory neuropeptides and their receptors participate in mechano-regulation of murine macrophages: D. Muschter, et al.; Int. J. Mol. Sci.
20, 503 (2019),
Application(s): ELISA using culture supernatants,
Abstract;
Full Text
Aggressive attempted escape behavior during head-lock restraint reduced reproductive performances in Holstein heifers: V.R. Kasimanickam, et al.; Theriogenology
121, 147 (2018),
Application(s): ELISA using bovine serum,
Abstract;
Effects of castration and a lidocaine-plus-flunixin treatment on growth and indicators of pain, inflammation, and liver function in Korean cattle bull calves: S.J. Park, et al.; Livestock Sci. 216, 61 (2018), Application(s): Bovine Plasma,
Predictors of beef calf temperament at weaning and its impact on temperament at breeding and reproductive performance: V.R. Kasimanickam, et al.; Reprod. Domest. Anim.
53, 484 (2018),
Application(s): ELISA using bovine serum,
Abstract;
Saliva as a medium to detect and measure biomarkers related to pain: H. Jasim, et al.; Sci. Rep.
8, 3220 (2018),
Application(s): ELISA using human plasma and saliva,
Abstract;
Full Text
Effects on breathing of agonists to μ-opioid or GABAA receptors dialyzed into the Ventral Respiratory Column of awake and sleeping goats: T.M. Langer, et al.; Respir. Physiol. Neurobiol.
239, 10 (2017),
Abstract;
Flunixin meglumine improves pregnancy rate in embryo recipient beef cows with an excitable temperament: R.K. Kasimanickam, et al.; Theriogenology
107, 70 (2017),
Abstract;
Full Text
Acid-Sensing Ion Channel 1a Contributes to Airway Hyperreactivity in Mice: L.R. Reznikov, et al.; PLoS One
11, e0166089 (2016),
Abstract;
Full Text
Efficacy of oral meloxicam suspension for prevention of pain and inflammation following band and surgical castration in calves: M. Olson, et al.; BMC Vet. Res.
12, 102 (2016),
Application(s): Measurment of substance P in blood samples,
Abstract;
Full Text
Evolution of Chronic Pain in Sickle Cell Disease: M. Pope, et al.; Blood
128, 1297 (2016),
Full Text
Substance P Receptor Signaling Mediates Doxorubicin-Induced Cardiomyocyte Apoptosis and Triple-Negative Breast Cancer Chemoresistance: P. Robinson, et al.; Biomed. Res. Int.
2016, 1959270 (2016),
Abstract;
Full Text
Substance P Receptor Signaling Mediates Doxorubicin-Induced Cardiomyocyte Apoptosis Triple-Negative Breast Cancer Chemoresistance: P. Robinson, et al.; Biomed. Res. Int.
2016, Article ID 1959270 (2016),
Application(s): Quantitation of SP Protein in cell lysates,
Abstract;
Full Text
The role of substance P in the maintenance of colonic hypermotility induced by repeated stress in rats: P. Lu, et al.; Neuropeptides
56, 75 (2016),
Application(s): Determine levels of substance P in serum,
Abstract;
Blockade of neurokinin-1 receptors in the ventral respiratory column does not affect breathing but alters neurochemical release: C. Muere, et al.; J. Appl. Physiol.
118, 732 (2015),
Application(s): ELISA,
Abstract;
Characteristics and Potential Biomarkers for Chronic Pain in Patients with Sickle Cell Disease: N. Kuei, et al.; Blood
126, 986 (2015),
Application(s): Human Substance P levels were measured,
Full Text
Neuropeptide release augments serum albumin loss and reduces ultrafiltration in peritoneal dialysis: N. Cavallini, et al.; Perit. Dial. Int.
32, 168 (2012),
Abstract;
Full Text
Effect of anti-NGF antibodies in a rat tibia fracture model of complex regional pain syndrome type I: J. Clark, et al. ; Pain
138, 47 (2008), Application(s): EIA using rat tissue (ref. 1),
Abstract;
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