-
Highly sensitive ELISA with an optional acetylation protocol which increases sensitivity 10-fold (0.025 pmol/ml)
-
Trusted and widely cited in peer-reviewed publications
-
Higher throughput format with results for up to 39 samples in duplicate
-
Simple, efficient, and well-established sample handling protocols
-
Reliable and consistent lot-to-lot performance
-
Save with bulk kit package!
This is a colorimetric competitive immunoassay kit for the quantitation of intracellular cGMP in cells or tissues lysed in 0.1 M HCl, which is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic GMP. The 0.1M HCl treated samples are then analyzed directly in a microtiter plate without extraction, drying and reconstitution. Sensitivity is increased by acetylation (reagents included). Absorbance is read at 405 nm.The ready-to-use liquid color-coded reagents provided reduce error.
Product Details
| Alternative Name: | cyclic GMP |
| |
| Sensitivity: | Non-acetylated format: 0.604 pmol/ml
Acetylated 2-hour format: 0.059 pmol/ml
Acetylated overnight format: 0.025 pmol/ml
Range: (non-acetylated): 0.8 - 500 pmol/ml
Range: (acetylated): 0.08 - 50 pmol/ml) |
| |
| Assay Time: | 3 hours |
| |
| Applications: | ELISA, Colorimetric detection
|
| |
| Application Notes: | For the quantitative determination of cGMP in cell lysates and tissue from any species. Cited sample types include cerebral spinal fluid and microdialysate. |
| |
| Wavelength: | 405 nm |
| |
| Species reactivity: | Species independent
|
| |
| Crossreactivity: | cyclic GMP (100%) and <0.001% GMP, GTP, cyclic AMP, AMP, ATP, cyclic UMP, CTP |
| |
| Use/Stability: | Store all components at +4°C, except standard and conjugate at -20°C. |
| |
| Shipping: | Shipped on Blue Ice |
| |
| Contents: | GxR Microtiter plate, Conjugate, Antibody, 0.1M HCl, Neutralizing reagent, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution, Acetic anhydride, Triethylamine |
| |
| Scientific Background: | Cyclic GMP (cGMP), which has been shown to be present at levels typically 10-100 fold lower than cAMP, is formed by the action of the enzyme guanylate cyclase on GTP. Some hormones, such as Acetylcholine, Insulin, and Oxytocin, as well as certain other chemicals like Serotonin and Histamine cause an increase in cGMP levels. Stimulators of guanylate cyclase such as the vasodilators nitroprusside, nitroglycerin, sodium nitrate, and nitric oxide (NO) also stimulate cGMP levels. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
 |
| |
Product Literature References
A Series of Substituted Bis-Aminotriazines Are Activators of the Natriuretic Peptide Receptor C: R.J. Smith, et al.; J. Med. Chem.
65, 5495 (2022),
Abstract;
Evaluation of Multivalent Enterotoxigenic Escherichia coli Vaccine Candidate MecVax Antigen Dose-Dependent Effect in a Murine Model: H. Seo, et al.; Appl. Environ. Microbiol.
88, e0095922 (2022),
Abstract;
MANP (M-Atrial Natriuretic Peptide) Reduces Blood Pressure and Furosemide-Induced Increase in Aldosterone in Hypertension: N.A. Dzhoyashvili, et al.; Hypertension (2022),
Abstract;
The role of CFA/I in adherence and toxin delivery by ETEC expressing multiple colonization factors in the human enteroid model: E.M. Smith, et al.; PLoS Negl. Trop. Dis.
16, e0010638 (2022),
Abstract;
Vaccine Candidate Double Mutant Variants of Enterotoxigenic Escherichia coli Heat-Stable Toxin: E.D. Zegeye, et al.; Vaccines
10, 241 (2022),
Abstract;
Ca2+ signals critical for egress and gametogenesis in malaria parasites depend on a multipass membrane protein that interacts with PKG: A.C. Balestra, et al.; Sci. Adv.
7, 5396 (2021),
Abstract;
Full Text
Discovery of small molecule guanylyl cyclase A receptor positive allosteric modulators: S.J. Sangarlingham, et al.; PNAS
118, e210938611 (2021),
Abstract;
Novel GUCY2C variant causing familial diarrhea in a Mennonite kindred and a potential therapeutic approach: R. Wolfe, et al.; Am. J. Med. Genet. A
185, 2046 (2021),
Abstract;
Angiotensin II represses Npr1 expression and receptor function by recruitment of transcription factors CREB and HSF-4a and activation of HDACs: K.K. Arise, et al.; Sci. Rep.
10, 4337 (2020),
Abstract;
Full Text
Characterization of ShigETEC, a Novel Live Attenuated Combined Vaccine against Shigellae and ETEC: S. Harutyunyan, et al.; Vaccines (Basel)
8, 689 (2020),
Abstract;
Full Text
Phosphodiesterase 5 (PDE5) Restricts Intracellular cGMP Accumulation During Enterotoxigenic Escherichia coli Infection: J. Foulke-Abel, et al.; Gut Microbes
12, 1752125 (2020),
Abstract;
Full Text
An unusual and vital protein with guanylate cyclase and P4-ATPase domains in a pathogenic protist: O. Gunay-Esiyok, et al.; Life Sci. Alliance
2, e201900402 (2019),
Abstract;
Full Text
Resveratrol treatment may preserve the erectile function after radiotherapy by restoring antioxidant defence mechanisms, SIRT1 and NOS protein expressions: T.E. Sener, et al.; Int. J. Impot. Res.
30, 179 (2018),
Abstract;
The role of cGMP as a mediator of lipolysis in bovine oocytes and its effects on embryo development and cryopreservation: K.R.L. Schwarz, et al.; PLoS One
13, e0191023 (2018),
Abstract;
Full Text
Involvement of second messengers in the signaling pathway of vitellogenesis-inhibiting hormone and their effects on vitellogenin mRNA expression in the whiteleg shrimp, Litopenaeus vannamei: S.H. Bae, et al.; Gen. Comp. Endocrinol.
246, 301 (2017),
Application(s): cGMP levels in shrimp ovaries,
Abstract;
Type 5 phosphodiesterase regulates glioblastoma multiforme aggressiveness and clinical outcome: V. Cesarini, et al.; Oncotarget
8, 13223 (2017),
Abstract;
Full Text
Differential expression and regulation of anti-hypertrophic genes Npr1 and Npr2 during β-adrenergic receptor activation-induced hypertrophic growth in rats: S. Manivasagam, et al.; Mol. Cell. Endocrinol.
433, 117 (2016),
Abstract;
Effects of Intermittent Fasting on Age-Related Changes on Na,K-ATPase Activity and Oxidative Status Induced by LPS In Rat Hippocampus: A.R. Vasconcelos, et al.; Neurobiol. Aging
36, 1914 (2015),
Application(s): ELISA using rat brain supernatant,
Abstract;
Full Text
PCSK6-mediated corin activation is essential for normal blood pressure: S. Chen, et al.; Nat. Med.
21, 1048 (2015),
Application(s): Intracellular cGMP concentrations in the cell lysate were measured,
Abstract;
Full Text
Sodium nitrite causes relaxation of the isolated rat aorta: By stimulating both endothelial NO synthase and activating soluble guanylyl cyclase in vascular smooth muscle: W.C. Ling, et al.; Vascul. Pharmacol.
74, 87 (2015),
Application(s): cGMP levels in rat aortic tissue,
Abstract;
Dephosphorylation and inactivation of NPR2 guanylyl cyclase in granulosa cells contributes to the LH-induced decrease in cGMP that causes resumption of meiosis in rat oocytes: J. Egbert, et al.; Development
141, 3594 (2013),
Application(s): EIA using rat ovarian follicles,
Abstract;
Nitric oxide synthase-3 promotes embryonic development of atrioventricular valves: Y. Liu, et al.; PLoS One
8, e77611 (2013),
Application(s): EIA using mouse cardiac tissue,
Abstract;
Full Text
Sestrin 2 and AMPK connect hyperglycemia to Nox4-dependent endothelial nitric oxide synthase uncoupling and matrix protein expression: A. Eid, et al.; Mol. Cell. Biol.
33, 3439 (2013),
Application(s): EIA using rat glomerular mesangial cells,
Abstract;
Full Text
β-Sitosterol oxidation products attenuate vasorelaxation by increasing reactive oxygen species and cyclooxygenase-2: C. Yang, et al.; Cardiovasc. Res.
97, 520 (2013),
Application(s): ELISA using rat aortic tissue,
Abstract;
Full Text
Chronic deficiency of nitric oxide affects hypoxia inducible factor-1α (HIF-1α) stability and migration in human endothelial cells: M.G. Cattaneo, et al.; PLoS One
6, e29680 (2011),
Application(s): EIA using human cell lysate,
Abstract;
Full Text
cGMP secreted from the tapeworm Hymenolepis diminuta is a signal molecule to the host intestine: N. Zimmerman, et al. ; J. Parasitol.
94, 771 (2008), Application(s): EIA using tapeworm culture supernatants,
Abstract;
Evidence for functional heterogeneity of circulating B-type natriuretic peptide: A. Protter, et al. ; J. Am. Coll. Cardiol.
49, 1071 (2007),
Application(s): EIA using human cell lysates,
Abstract;
Estradiol and progesterone modulate the nitric oxide/cyclic gmp pathway in the hypothalamus of female rats and in GT1-1 cells: H.P. Chu, et al. ; Endocrine
24, 177 (2004), Application(s): EIA using rat cell lysates,
Abstract;
A Novel PDZ Protein Regulates the Activity of Guanylyl Cyclase C, the Heat-stable Enterotoxin Receptor: R.O. Scott, et al. ; J. Biol. Chem.
277, 22934 (2002), Application(s): EIA using monkey cell lysates,
Abstract;
The Mechanism of Ciliary Stimulation by Acetylcholine: Roles of Calcium, PKA, and PKG: O. Zagoory, et al. ; J. Gen. Physiol.
119, 329 (2002),
Application(s): EIA using frog cell lysates,
Abstract;
Differential nitric oxide synthase activity, cofactor availability and cGMP accumulation in the central nervous system during anaesthesia: H.F. Galley, et al. ; Br. J. Anaesth.
86, 388 (2001), Application(s): EIA using rat cell lysates,
Abstract;
Mechanism underlying diuretic effect of L-NAME at a subpressor dose: M. Liang, et al. ; Ren. Physiol.
281, F414 (2001),
Application(s): EIA using rat microdialysate,
Abstract;
Full Text
The plasma membrane calmodulin-dependent calcium pump: a major regulator of nitric oxide synthase I: K. Schuh, et al. ; J. Cell Biol.
155, 201 (2001),
Application(s): EIA using human cell lysates,
Abstract;
Role of cGMP versus 20-HETE in the vasodilator response to nitric oxide in rat cerebral arteries: C.W. Sun, et al. ; Am. J. Physiol. Heart Circ. Physiol.
279, H339 (2000), Application(s): EIA using rat cell lysates,
Abstract;
Increased cerebrospinal fluid cAMP levels in Alzheimer's disease: M. Martinez, et al.; Brain Res.
846, 265 (1999),
Application(s): EIA using human cerbrospinal fluid,
Abstract;
Related Products
