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Progesterone ELISA kit

ADI-901-011 5x96 wells 1,490.00 USD
Do you need bulk/larger quantities?
Alternative size available: ADI-900-011 (1x96 wells)
  • High sensitivity, measuring as little as 8.57 pg/ml of progesterone
  • Highly specific with negligible cross-reactivity to other steroids
  • High throughput format with results in < 3 hours for up to 38 samples in duplicate
  • Easy-to-use liquid color-coded reagents reduce errors
  • Save with bulk kit package!
The Progesterone EIA kit is a colorimetric competitive enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 405 nm. This non-radioactive enzyme immunoassay is safe and reliable with ready-to-use liquid color-coded reagents to reduce errors.

Product Details

Sensitivity:8.57 pg/ml (range 15.62 - 500 pg/ml)
Assay Time:<3 hours
Applications:ELISA, Colorimetric detection
Application Notes:For the quantitative determination of progesterone in culture supernatants, serum, and saliva from any species. Cited sample type includes plasma.
Wavelength:405 nm
Species reactivity:Species independent
Shipping:Blue Ice
Long Term Storage:+4°C
Contents:GxM Microtiter plate, Conjugate, Antibody, Assay buffer concentrate, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution, Steroid displacement reagent
Scientific Background:Progesterone is the major female sex hormone, and is responsible for reproductive-related activities such as breast glandular development, the endometrial aspects of the menstrual cycle, and the establishment and maintenance of pregnancy. In addition, progesterone also directs pregnancy support physiology including changes in carbohydrate, protein and lipid metabolism, thermoregulation, sodium reabsorption in renal tubules and the reduction of alveolar and atrial carbon dioxide partial pressures (pCO2). Progesterone is secreted in large amounts by the corpus luteum and by the extracellular conversion of cholesterol, cholestryl esters, adrenal steroids, prenenolone and pregnenolone sulfate. Small quantities are also secreted directly from the adrenal glands.
Technical Info/Product Notes:Cited samples:
Hormone ELISAs: Cited Samples
Wildlife Endocrinology: Cited Sample Types

Application Note:
Validation of Two Steroid Hormone ELISA Kits with Ovine Blood Samples.
Regulatory Status:RUO - Research Use Only
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

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Product Literature References

Estrogen and progesterone receptors and antioxidant enzymes are expressed differently in the uterus of domestic cats during the estrous cycle: A.E. de Jesus Nascimento, et al.; Theriogenology 203, 1 (2023), Abstract;
Pregnancy rate and reproductive hormones in humpback whale blubber: Dominant form of progesterone differs during pregnancy: S. Atkinson, et al.; Gen. Comp. Endocrinol. 330, 114151 (2023), Abstract;
Stress and reproductive hormones in hair associated with contaminant metal(loid)s of European brown bear (Ursus arctos): M. Lazarus, et al.; Chemosphere 325, 138354 (2023), Abstract;
Synergistic effect of standardized extract of Asparagus officinalis stem and heat shock on progesterone synthesis with lipid droplets and mitochondrial function in bovine granulosa cells: K.T. Ho, et al.; J. Steroid Biochem. Mol. Biol. 225, 106181 (2023), Abstract;
Changes in concentrations of cervicovaginal immune mediators across the menstrual cycle: a systematic review and meta-analysis of individual patient data: S.M. Hughes, et al.; BMC Med. 20, 353 (2022), Abstract;
Deficiency of Wnt10a causes female infertility via the β-catenin/Cyp19a1 pathway in mice: J.H. Zhang, et al.; Int. J. Med. Sci. 19, 701 (2022), Abstract;
Demography of an ice-obligate mysticete in a region of rapid environmental change: L. Pallin, et al.; R. Soc. Open Sci. 9, 220724 (2022), Abstract;
Effects of vessel traffic and ocean noise on gray whale stress hormones: L.S. Lemos, et al.; Sci. Rep. 12, 18580 (2022), Abstract;
Estrous cycle contributes to state-dependent contextual fear in female rats: R.S. Blair, et al.; Psychoneuroendocrinology 141, 105776 (2022), Abstract;
Low plasma progesterone concentration during the early luteal phase delays endometrial development and the beginning of placentation in mares: C.T.C. Okada, et al.; Anim. Reprod. Sci. 247, 107149 (2022), Abstract;
MEHP induces alteration of mitochondrial function and inhibition of steroid biosynthesis in MA-10 mouse tumor Leydig cells: K. Traore, et al.; Toxicology 463, 152985 (2022), Abstract;
Neuroendocrine and neuroimmune responses in male and female rats: Evidence for functional immaturity of the neuroimmune system during early adolescence: P. Marsland, et al.; Eur. J. Neurosci. 55, 2311 (2022), Abstract;
Non-invasive assessment of fecal glucocorticoid, progesterone, and androgen metabolites and microbiome in free-ranging southern white rhinoceros (Ceratotherium simum simum) in South Africa: K.H. Kothmann, et al.; Gen. Comp. Endocrinol. 329, 114099 (2022), Abstract;
Testicular Macrophages Produce Progesterone De Novo Promoted by cAMP and Inhibited by M1 Polarization Inducers: S. Yamauchi, et al.; Biomedicines 10, 487 (2022), Abstract;
The anxiolytic effect of koumine on a predatory sound stress-induced anxiety model and its associated molecular mechanisms: B. Xiong, et al.; Phytomedicine 103, 154225 (2022), Abstract;
Variation in blubber cortisol levels in a recovering humpback whale population inhabiting a rapidly changing environment: L.J. Pallin, et al.; Sci. Rep. 12, 20250 (2022), Abstract;
A standardized extract of Asparagus officinalis stem improves HSP70-mediated redox balance and cell functions in bovine cumulus-granulosa cells: K.T. Ho, et al.; Sci. Rep. 11, 18175 (2021), Abstract;
Antidepressant-like effects of Z-ligustilide on chronic unpredictable mild stress-induced depression in rats: J.C. Ma, et al.; Exp. Ther. Med. 22, 677 (2021), Abstract;
Chronic irisin exposure decreases sexual incentive motivation in female rats: N. Ulker, et al.; Physiol. Behav. 232, 113341 (2021), Application(s): Rat serum, Abstract;
Endometrial Inflammation at the Time of Insemination and Its Effect on Subsequent Fertility of Dairy Cows: K. Wagener, et al.; Animals 11, 1858 (2021), Abstract;
Hormone autocrination by vascularized hydrogel delivery of ovary spheroids to rescue ovarian dysfunctions: H.J. Yoon, et al.; Sci. Adv. 7, eabe8873 (2021), Abstract;
Impact of heat stress on embryonic development during first 16 days of gestation in dairy cows: R. Kasimanickam & V. Kasimanickam; Sci. Rep. 11, 14839 (2021), Abstract;
Kisspeptin/Kiss1r system and angiogenic and immunological mediators at the maternal-fetal interface of domestic cats: L.C. Santos, et al.; Biol. Reprod. 61, 1093 (2021), Abstract;
Organochlorine pesticide dieldrin upregulate proximal promoter (PII) driven CYP19A1 gene expression and increases estrogen production in granulosa cells: D. Sharma, et al.; Reprod. Toxicol. 106, 103 (2021), Abstract;
Tri-ortho-cresyl phosphate exposure leads to low egg production and poor eggshell quality via disrupting follicular development and shell gland function in laying hens: J. Li, et al.; Ecotoxicol. Environ. Saf. 225, 112771 (2021), Abstract;
Urinary equol levels are positively associated with urinary estradiol excretion in women: T. Fujitani, et al.; Sci. Rep. 11, 19532 (2021), Abstract;
Assessment of fecal steroid and thyroid hormone metabolites in eastern North Pacific gray whales: L.S. Lemos, et al.; Conserv. Physiol. 8, coaa110 (2020), Application(s): Whale feces, Abstract; Full Text
Encapsulation of Mesenchymal Stem Cells in 3D Ovarian Cell Constructs Promotes Stable and Long-Term Hormone Secretion with Improved Physiological Outcomes in a Syngeneic Rat Model: S. Sittadjody, et al.; Ann. Biomed. Eng. 48, 1058 (2020), Application(s): Medium Supernatant from Rat Biometric Ovarian Constructs, Abstract; Full Text
A heterozygous deficiency in protein phosphatase Ppm1b results in an altered ovulation number in mice: N. Ishii, et al.; Mol. Med. Rep. 19, 5353 (2019), Application(s): ELISA using mouse plasma, Abstract;
Anti-Müllerian hormone and progesterone levels in human follicular fluid are predictors of embryonic development: Y. O'Brien, et al.; Reprod. Biol. Endocrinol. 17, 47 (2019), Application(s): ELISA using human follicular fluid, Abstract; Full Text
Anxiolytic- and anxiogenic-like effects of Montanoa tomentosa (Asteraceae): Dependence on the endocrine condition: E. Estrada-Camarena, et al.; J. Ethnopharmacol. 241, 112006 (2019), Abstract;
Effects of dietary L-arginine supplementation to early pregnant mares on conceptus diameter-Preliminary findings: J. Aurich, et al.; Reprod. Domest. Anim. 54, 772 (2019), Application(s): ELISA using horse plasma, Abstract;
Evidence for Increased Content of PGF2α, PGE2, and 6-keto-PGF1α in Endometrial Tissue Cultures from Heavy Draft Mares in Anestrus With Endometritis: J.M. Siemieniuch, et al.; J. Equine Vet. Sci. 77, 107 (2019), Abstract;
External stress increases sympathoadrenal activity and prolongs the expulsive phase of foaling in pony mares: M. Melchert, et al.; Theriogenology 128, 110 (2019), Application(s): ELISA using horse plasma, Abstract;
Insights on the seasonal variations of reproductive features in the Eastern Atlantic Bluefin Tuna: O. Carnevali, et al.; Gen. Comp. Endocrinol. 282, 113216 (2019), Application(s): Bluefin Tuna blood plasma, Abstract;
Stimulation of nicotinic receptors in the suprachiasmatic nucleus results in a higher number of growing follicles and ova shed: E. Vieyra, et al.; Exp. Physiol. 104, 1179 (2019), Application(s): ELISA using rat serum, Abstract;
Tracking transcriptomic responses to endogenous and exogenous variation in cetaceans in the Southern California Bight: M.L. Trego, et al.; Conserv. Physiol. 7, coz018 (2019), Application(s): ELISA using dolphin blubber, Abstract; Full Text
Can concentrations of steroid hormones in brown bear hair reveal age class?: M. Cattet, et al.; Conserv. Physiol. 6, coy001 (2018), Application(s): ELISA using brown bear hair samples, Abstract; Full Text
Computational repositioning and preclinical validation of mifepristone for human vestibular schwannoma: J.E. Sagers, et al.; Sci. Rep. 8, 5437 (2018), Application(s): ELISA using culture supernatants, Abstract; Full Text
Early luteal phase progestin concentration influences endometrial function in pregnant mares: T. Beyer, et al.; Theriogenology 125, 236 (2018), Abstract;
Effect of molecular hydrogen on uterine inflammation during preterm labour: T. Nakano, et al.; Biomed. Rep. 8, 454 (2018), Application(s): ELISA using mouse serum, Abstract; Full Text
Environmental enrichment reverses Aβ pathology during pregnancy in a mouse model of Alzheimer's disease: S. Ziegler-Waldkirch, et al.; Acta Neuropathol. Commun. 6, 44 (2018), Abstract; Full Text
Estrogen and progesterone integration in an in vitro model of RP3V kisspeptin neurons: M.A. Mittelman-Smith, et al.; Neuroendocrinology 106, 101 (2018), Application(s): ELISA using culture supernatants, Abstract; Full Text
Oxidative and endoplasmic reticulum stress defense mechanisms of bovine granulosa cells exposed to heat stress: T.W. Alemu, et al.; Theriogenology 110, 130 (2018), Abstract;
The consequences of porcine IVM medium supplementation with follicular fluid become reflected in embryo quality, yield and gene expression patterns: P. Pawlak, et al.; Sci. Rep. 8, 15306 (2018), Abstract; Full Text
Validation of a blubber-based endocrine pregnancy test for humpback whales: L. Pallin, et al.; Conserv. Physiol. 6, coy031 (2018), Abstract; Full Text
Advanced age in mares affects endometrial secretion of arachidonic acid metabolites during equine subclinical endometritis: M.J. Siemieniuch, et al.; Theriogenology 103, 191 (2017), Application(s): ELISA using horse serum, Abstract;
Effect of antipsychotics on mitochondrial bioenergetics of rat ovarian theca cells: E. Elmorsy, et al.; Toxicol. Lett. 272, 92 (2017), Application(s): ELISA using culture supernatant, Abstract;
Endometrial mRNA expression of selected pro-inflammatory factors and mucins in repeat breeder cows with and without subclinical endometritis: K. Wagener, et al.; Theriogenology 90, 237 (2017), Application(s): ELISA using bovine plasma, Abstract;
In vivo transplantation of 3D encapsulated ovarian constructs in rats corrects abnormalities of ovarian failure: S. Sittajody, et al.; Nat. Commun. 8, 1858 (2017), Abstract; Full Text
Loss of predator aversion in female rats after Toxoplasma gondii infection is not dependent on ovarian steroids: S. Abdulai-Saiku & A. Vyas; Brain Behav. Immun. 65, 95 (2017), Application(s): ELISA using male rat samples with T. Gondi infection , Abstract;
Progesterone Modifies the Responsivity of the Amygdala-mPFC Connection in Male but not Female Wistar Rats: C.M. Contreras, et al.; Neurosci. Lett. 649, 1 (2017), Application(s): ELISA using rat plasma samples, Abstract;
Puerarin ameliorated the behavioral deficits induced by chronic stress in rats: Z.K. Qiu, et al.; Sci. Rep. 7, 6266 (2017), Application(s): EIA on rat tissue homogenate, Abstract; Full Text
Application of an innovative technique for unilateral ovariectomy in dairy cows: A. Rizzo, et al.; Vet. Rec. 179, 463 (2016), Abstract;
Does BPA alter steroid hormone synthesis in human granulosa cells in vitro?: A. Mansur, et al.; Hum. Reprod. 31, 1562 (2016), Application(s): Medium supernatant from human mural granulosa cells, Abstract;
Effects of periovulatory gonadotrophin treatment on luteal function and endometrial expression of selected genes in cyclic pony mares: M. Köhne, et al.; Theriogenology 86, 2147 (2016), Application(s): ELISA using horse serum, Abstract;
Implantation and pregnancy outcome of Sprague-Dawley rats fed with low and high salt diet: G.O. Oludare, et al.; Middle East Fertil. Soc. J. 21, 228 (2016), Application(s): Measured rat serum progesterone levels,
Initiating Ovsynch on day 6 post estrus onset ± post AI early luteal phase GnRH treatment to improve ovarian and fertility response in repeat breeding crossbred cattle: N. Ahmed, et al.; Indian J. Anim. Reprod. 37, 5 (2016), Application(s): Serum progesterone ELISA, Full Text
Intravenous Immunoglobulin Suppresses Abortion Relates To an Increase in the CD44bright NK Subset in Recurrent Pregnancy Loss Model Mice: J. Tanaka, et al.; Biol. Reprod. 116, 138438 (2016), Application(s): Measurement of plasma P4 concentration and effect on peripheral immune cells, Abstract; Full Text
Maternal and fetal roles in bacterially induced preterm labor in the mouse: Y. Filipovich, et al.; Am. J. Obstet. Gynecol. 214, 386 (2016), Application(s): ELISA using mouse serum, Abstract; Full Text
Prostaglandin release by cultured endometrial tissues after challenge with lipopolysaccharide and tumor necrosis factor α, in relation to the estrous cycle, treatment with medroxyprogesterone acetate, and pyometra: E. Jursza-Piotrowska, et al.; Theriogenology 85, 1177 (2016), Abstract;
REGULATORY ROLE OF miR-20a DURING BOVINE OOCYTE MATURATION: E. Andreas, et al.; Reprod. Fertil. Dev. 29, 196 (2016), (Meeting abstract); Application(s): Bovine oocyte media, Full Text
Standard human chorionic gonadotropin versus double trigger for final oocyte maturation results in different granulosa cells gene expressions: a pilot study: J. Haas, et al.; Fertil. Steril. 106, 653 (2016), Application(s): Human Follicular Fluid, Abstract;
Testosterone production by a leydig tumor cell line is suppressed by hyperthermia-induced endoplasmic reticulum stress in mice: J.H. Kim, et al.; Life Sci. 146, 184 (2016), Application(s): Measurement of progesterone in mouse mLTC-1 cell culture media, Abstract;
The PGF2α agonists luprostiol and d-cloprostenol reliably induce luteolysis in luteal phase mares without evoking clinical side effects or a stress response: J. Kuhl, et al.; Anim. Reprod. Sci. 168, 92 (2016), Application(s): Progesterone levels were measured in blood samples, Abstract;
The physiological expression of scavenger receptor SR-B1 in canine endometrial and placental epithelial cells and its potential involvement in pathogenesis of pyometra: C. Gabriel, et al.; Theriogenology 85, 1599 (2016), Application(s): Measured canine serum progesterone levels, Abstract;
Type of Inflammation Differentially Affects Expression of Interleukin 1β and 6, Tumor Necrosis Factor-α and Toll-Like Receptors in Subclinical Endometritis in Mares: M.J. Siemieniuch, et al.; PLoS One 11, e0154934 (2016), Application(s): Horse serum progesterone levels were measured, Abstract; Full Text
Altered secretion of selected arachidonic acid metabolites during subclinical endometritis relative to estrous cycle stage and grade of fibrosis in mares: K. Gajos, et al.; Theriogenology 84, 457 (2015), Application(s): ELISA using mare plasma, Abstract;
Determining intrafollicular concentrations of cortisol and progesterone in horses and the effects of cortisol on in vitro maturation of equine oocytes: D. Scarlet, et al. ; SBTE & AETE Meeting Abstracts (2015), Application(s): ELISA using horse samples, Full Text
Effects of season, age, sex and housing on salivary cortisol concentrations in horses: J. Aurich, et al.; Domest. Anim. Endocrinol. 52, 11 (2015), Application(s): ELISA, Abstract;
The prevalence of subclinical endometritis and intrauterine infections in repeat breeder cows: H. Pothmann, et al.; Theriogenology 83, 1249 (2015), Application(s): ELISA using cow plasma, Abstract;
Use of an anti-GnRF vaccine to suppress oestrus in crossbred Iberian female pigs: A. Dalmau, et al.; Theriogenology 84, 342 (2015), Application(s): ELISA using porcine serum , Abstract;
Uterine blood flow indices, antinuclear autoantibodies and unexplained recurrent miscarriage: A. Pietropolli, et al.; Obstet. Gynecol. Sci. 58, 453 (2015), Application(s): Measurement of human serum progesterone levels, Abstract;
Establishment and validation of a model for non-luteinized human mural granulosa cell culture: L. Ophir, et al.; Mol. Cell. Endocrinol. 384, 165 (2014), Application(s): EIA using culture supernatants from human cells, Abstract;
Placental origin of prostaglandin F2α in the domestic cat: M.J. Siemieniuch, et al.; Mediators Inflamm. 2014, 364787 (2014), Application(s): ELISA using feline placenta, Abstract; Full Text
Progesterone antagonist, RU486, represses LHCGR expression and LH/hCG signaling in cultured luteinized human mural granulosa cells: Y. Yung, et al.; Gynecol. Endocrinol. 30, 42 (2014), Application(s): EIA using culture supernatants from human cells, Abstract;
Treatment with human chorionic gonadotrophin before ovulation increases progestin concentration in early equine pregnancies: M. Köhne, et al.; Anim. Reprod. Sci. 149, 187 (2014), Application(s): ELISA using horse plasma, Abstract;
Tumor endothelial marker 1–specific DNA vaccination targets tumor vasculature: J. Facciponte, et al.; J. Clin. Invest. 124, 1497 (2014), Application(s): EIA using mouse serum, Abstract; Full Text
Cystic ovarian follicles and thyroid activity in the dairy cow: M. Mutinati, et al.; Anim. Reprod. Sci. 138, 150 (2013), Application(s): EIA using bovine serum, Abstract;
Engineered multilayer ovarian tissue that secretes sex steroids and peptide hormones in response to gonadotropins: S. Sittadjody, et al.; Biomaterials 34, 2412 (2013), Application(s): ELISA using supernatant of cultured primary rat ovaries, Abstract; Full Text
Female preference for males depends on reproductive physiology in the African cichlid fish Astatotilapia burtoni: M.R. Kidd, et al.; Gen. Comp. Endocrinol. 180, 56 (2013), Application(s): ELISA using aquarium water, Abstract;
Follicular stage-dependent regulation of apoptosis and steroidogenesis by prohibitin in rat granulosa cells: Q. Wang, et al.; J. Ovarian Res. 6, 23 (2013), Application(s): ELISA using supernatant of rat granulosa cells , Abstract; Full Text
Sex differences and similarities in the neuroendocrine regulation of social behavior in an African cichlid fish: L.A. O'Connell, et al.; Horm. Behav. 64, 468 (2013), Application(s): EIA using fish plasma, Abstract;
Sex differences in the effects of adolescent stress on adult brain inflammatory markers in rats: L.M. Pyter, et al.; Brain Behav. Immun. 30, 88 (2013), Application(s): EIA using rat plasma, Abstract; Full Text
The screening of everyday life chemicals in validated assays targeting the pituitary-gonadal axis: H. Tinwell, et al.; Regul. Toxicol. Pharmacol. 66, 184 (2013), Application(s): EIA using rat plasma, Abstract;
Variation of bowhead whale progesterone concentrations across demographic groups and sample matrices: N.M. Kellar, et al.; Endanger. Species Res. 22, 61 (2013), Application(s): Serum, Urine and Blubber from Bowhead Whales, Full Text
Social status predicts how sex steroid receptors regulate complex behavior across levels of biological organization: L.A. O'Connell, et al.; Endocrinology 153, 1341 (2012), Application(s): EIA using fish plasma, Abstract;
Fecal Hormones Measured within Giant Pacific Octopuses Enteroctopus dofleini: S. Larson & R. Anderson; J. Aquat. Anim. Health 22, 152 (2010), Application(s): EIA using octopus feces, Abstract;
Measuring multiple hormones from a single water sample using enzyme immunoassays: C. Kidd, et al.; Gen. Comp. Endocrinol. 165, 277 (2010), Application(s): EIA using fish plasma and water samples, Abstract;

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