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17β-Estradiol ELISA kit

Highly sensitive ELISA, measuring as little as 28.5 pg/ml of 17β-Estradiol in < 3 hours
 
ADI-901-008 5x96 wells 1,244.00 USD
Do you need bulk/larger quantities?
 
Alternative size available: ADI-900-008 (1x96 wells)
  • Specific with low cross-reactivity to related steroids
  • Safe and non-radioactive enzymatic assay
  • Simple and color-coded reagents reduces error
  • Reliable and reproducible results day-after-day and lot-after-lot
  • Fully quantitative results that surpass semi-quantitative Western blot analysis
  • Save with bulk kit package!
The 17β-Estradiol EIA kit is a colorimetric competitive enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 405 nm. This kit offers specific and save non-radioactive quantification of estradiol with ready-to-use liquid color-coded reagents to reduce error.
17&beta;-Estradiol ELISA kit Standard curve
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17&beta;-Estradiol ELISA kit Standard curve

Product Details

Alternative Name:Estrogen
 
Sensitivity:28.5 pg/ml (range 29.3 - 30,000 pg/ml)
 
Assay Time:<3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of 17β-Estradiol in culture supernatants and saliva from any species. For serum and plasma use ADI-901-174.
 
Wavelength:405 nm
 
Species reactivity:Species independent
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Contents:GxR Microtiter plate, Conjugate, Antibody, Assay buffer concentrate, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution, Steroid displacement reagent
 
Scientific Background:Biologically active estrogen, 17β-estradiol, is an important sex hormone, but also makes an important contribution to the high concentrations of estrogens, which are present in malignant breast tissues.
 
Technical Info/Product Notes:Cited samples:
Hormone ELISAs: Cited Samples
Wildlife Endocrinology: Cited Sample Types
 
Regulatory Status:RUO - Research Use Only
 
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

Plate Reader Just Got Personal
 

Product Literature References

Deficiency of Wnt10a causes female infertility via the β-catenin/Cyp19a1 pathway in mice: J.H. Zhang, et al.; Int. J. Med. Sci. 19, 701 (2022), Abstract;
A novel method to differentiate tonsil-derived mesenchymal stem cells in vitro into estrogen-secreting cells: H. Kim, et al.; Tissue Eng. Regen. Med. 18, 253 (2021), Application(s): ELISA using culture supernatants, Abstract; Full Text
Chronic irisin exposure decreases sexual incentive motivation in female rats: N. Ulker, et al.; Physiol. Behav. 232, 113341 (2021), Application(s): Rat serum, Abstract;
Hormone autocrination by vascularized hydrogel delivery of ovary spheroids to rescue ovarian dysfunctions: H.J. Yoon, et al.; Sci. Adv. 7, eabe8873 (2021), Abstract;
Kisspeptin/Kiss1r system and angiogenic and immunological mediators at the maternal-fetal interface of domestic cats: L.C. Santos, et al.; Biol. Reprod. 61, 1093 (2021), Abstract;
Neonatal androgenization in rats affects oocyte maturation: G. Anesetti & R. Chávez-Genaro; Reprod Sci. 28, 2799 (2021), Abstract;
Organochlorine pesticide dieldrin upregulate proximal promoter (PII) driven CYP19A1 gene expression and increases estrogen production in granulosa cells: D. Sharma, et al.; Reprod. Toxicol. 106, 103 (2021), Abstract;
Encapsulation of mesenchymal stem cells in 3D ovarian cell constructs promotes stable and long-term hormone secretion with improved physiological outcomes in a syngeneic rat model: S. Sittadjody, et al.; Ann. Biomed. Eng. 48, 1058 (2020), Application(s): ELISA using culture supernatants, Abstract; Full Text
A high level of TGF-B1 promotes endometriosis development via cell migration, adhesiveness, colonization, and invasiveness: U.K. Soni, et al.; Biol. Reprod. 100, 917 (2019), Abstract;
CB1 agonism prolongs therapeutic window for hormone replacement in ovariectomized mice: K. Zhang, et al.; J. Clin. Invest. 130, 2333 (2019), Abstract; Full Text
Hormonal balance and nutritional intake in elite tactical athletes: A.E. Jensen, et al.; Steroids 15, 108504 (2019), Application(s): ELISA using human plasma, Abstract;
Maternal nicotinamide riboside enhances postpartum weight loss, juvenile offspring development, and neurogenesis of adult offspring: P.H. Ear, et al.; Cell Rep. 26, 969 (2019), Application(s): ELISA using mouse and rat plasma samples, Abstract;
Effects of Supplementation of Human Endometriotic Fluids on In Vitro Mouse Preantral Follicle Culture: S.K. Kim, et al.; Reprod Sci. 25, 5 (2018), Abstract;
Inflammatory Responses are Sex Specific in Chronic Hypoxic-Ischemic Encephalopathy: A. Al Mamun, et al.; Cell Transplant. 27, 1328 (2018), Application(s): EIA using human serum, Abstract; Full Text
Welsh Onion Root (Allium fistulosum) Restores Ovarian Functions from Letrozole Induced-Polycystic Ovary Syndrome: Y.H. Lee, et al.; Nutrients 10, 1430 (2018), Application(s): Rat serum analysis, Abstract; Full Text
Dibutyl phthalate impairs steroidogenesis and a subset of LH-dependent genes in cultured human mural granulosa cell in vitro: M. Adir, et al.; Reprod. Toxicol. 69, 13 (2017), Application(s): Medium supernatant from human mural granulosa cells, Abstract;
Estrogen activation of microglia underlies the sexually dimorphic differences in Nf1 optic glioma-induced retinal pathology: J.A. Toonen, et al.; J. Exp. Med. 214, 17 (2017), Application(s): ELISA for human samples, Abstract; Full Text
Implantation and pregnancy outcome of Sprague-Dawley rats fed with low and high salt diet: G.O. Oludare, et al.; Middle East Fertil. Soc. J. 21, 228 (2016), Application(s): Measured rat serum estradiol levels,
Long-Term High Fat Diet Has a Profound Effect on Body Weight, Hormone Levels, and Estrous Cycle in Mice: T.R. Chacraborty, et al.; Med. Sci. Monit. 22, 1601 (2016), Application(s): ELISA using mouse serum, Abstract; Full Text
Optimizing multi-dimensional high throughput screening using zebrafish: L. Truong, et al.; Reprod. Toxicol. 65, 139 (2016), Application(s): Nominal exposure concentration measurement of 17β-estradiol and 17α-ethinylestradiol, Abstract;
Standard human chorionic gonadotropin versus double trigger for final oocyte maturation results in different granulosa cells gene expressions: a pilot study: J. Haas, et al.; Fertil. Steril. 106, 653 (2016), Application(s): Human Follicular Fluid, Abstract;
Automated and portable solid phase extraction platform for immuno-detection of 17β-estradiol in water: S. Heub, et al.; J. Chromatogr. A. 1381, 22 (2015), Application(s): ELISA using water samples, Abstract;
Arsenic-induced cancer cell phenotype in human breast epithelia is estrogen receptor-independent but involves aromatase activation: Y. Xu, et al.; Arch. Toxicol. 88, 263 (2014), Application(s): ELISA using culture supernatant, Abstract; Full Text
Estradiol treatment promotes cardiac stem cell (CSC)-derived growth factors, thus improving CSC-mediated cardioprotection after acute ischemia/reperfusion: L. Wang, et al.; Surgery 156, 243 (2014), Application(s): ELISA using culture supernatant and cell perfusate solution, Abstract;
Expression and activity of Rac1 is negatively affected in the dehydroepiandrosterone induced polycystic ovary of mouse: V. Maurya, et al.; J. Ovarian Res. 7, 32 (2014), Application(s): EIA using mouse ovarian tissue, Abstract; Full Text
GnRH Agonist vs. hCG for Triggering of Ovulation – Differential Effects on Gene Expression in Human Granulosa Cells: J. Haas, et al.; PLoS One 9, e90359 (2014), Application(s): Human Serum, Abstract; Full Text
Tumor endothelial marker 1–specific DNA vaccination targets tumor vasculature: J. Facciponte, et al.; J. Clin. Invest. 124, 1497 (2014), Application(s): EIA using mouse serum, Abstract; Full Text
Engineered multilayer ovarian tissue that secretes sex steroids and peptide hormones in response to gonadotropins: S. Sittadjody, et al.; Biomaterials 34, 2412 (2013), Application(s): ELISA using supernatant of cultured primary rat ovaries, Abstract; Full Text
Female preference for males depends on reproductive physiology in the African cichlid fish Astatotilapia burtoni: M.R. Kidd, et al.; Gen. Comp. Endocrinol. 180, 56 (2013), Application(s): ELISA using aquarium water, Abstract;
Follicular stage-dependent regulation of apoptosis and steroidogenesis by prohibitin in rat granulosa cells: Q. Wang, et al.; J. Ovarian Res. 6, 23 (2013), Application(s): ELISA using supernatant of rat granulosa cells , Abstract; Full Text
Gender dimorphism in regulation of plasma proteins in streptozotocin-induced diabetic rats: J.W. Choi, et al.; Proteomics 13, 2482 (2013), Application(s): Rat plasma, Abstract;
MEN1 is a melanoma tumor suppressor that preserves genomic integrity by stimulating transcription of genes that promote homologous recombination-directed DNA repair: M. Fang, et al.; Mol. Cell. Biol. 33, 2635 (2013), Application(s): ELISA using cell lysates, Abstract; Full Text
Preliminary findings of fecal gonadal hormone concentrations in six captive sea otters (Enhydra lutris) after deslorelin implantation: S. Larson, et al.; Zoo Biol. 32, 307 (2013), Application(s): EIA using sea otter feces, Abstract;
Sex steroid hormones modulate responses to social challenge and opportunity in males of the monogamous convict cichlid, Amatitliana nigrofasciata: A. Sessa, et al.; Gen. Comp. Endocrinol. 189, 59 (2013), Application(s): EIA using fish plasma, Abstract;
Fecal Hormones Measured within Giant Pacific Octopuses Enteroctopus dofleini: S. Larson & R. Anderson; J. Aquat. Anim. Health 22, 152 (2010), Application(s): EIA using octopus feces, Abstract;
Measuring multiple hormones from a single water sample using enzyme immunoassays: C. Kidd, et al.; Gen. Comp. Endocrinol. 165, 277 (2010), Application(s): EIA using fish plasma and water samples, Abstract;
Sex steroid-mediated reprogramming of vascular smooth muscle cells to stem cells and neurons: possible utilization of sex steroid combinations for regenerative treatment without utilization of in vitro developed stem cells: A. Bukovsky; Cell Cycle 8, 4079 (2009), Application(s): EIA using ovarian epithelial cells, Abstract;
Comparison of three enzyme immunoassays for measuring 17beta-estradiol in flushed dairy manure wastewater: T. Hanselman, et al.; J. Environ. Qual. 33, 1919 (2004), Application(s): EIA using livestock feces, water samples, Abstract;

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