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Sensitive ELISA, detecting as little as 2.01 ng/ml of human plasminogen
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Flexible sample types, including: cell lysates, plasma, and serum
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Simple protocol with results in just 3.5 hours
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Quantitative results as compared to Western blot analysis
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Low cross reactivity to similar molecules
The Plasminogen (human) ELISA kit is a complete, colorimetric, immunometric immunoassay kit for the quantitative determination of human plasminogen I in cell lysate, plasma, and serum samples with results in about 3.5 hours.
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Product Specification
| Sensitivity: | <2.01 ng/ml (range 1 - 64 ng/ml) |
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| Assay Time: | ~3.5 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of human plasminogen in cell lysate, plasma, and serum samples. |
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| Wavelength: | 450 nm |
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| Species reactivity: | Human
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| Crossreactivity: | No cross reactivity to similar molecules (human serum albumin, transferrin, IgG, α-fetoprotein (AFP), fibrinogen, vitamin D binding protein (VDBP), hemoglobin). |
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| Handling: | All kit components are stable at 2 to 8°C. Any unused reconstituted standard should be discarded or frozen at -70°C. Standard can be frozen and thawed one time only without loss of immunoreactivity. |
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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| Contents: | Microtiter Plate, Incubation Buffer, Washing Buffer, Protein Standard, Dilution Buffers, Secondary Antibody, AV-HRP, TMB Substrate, Stop Solution |
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| Scientific Background: | Plasminogen, a 92kDa glycoprotein, is produced by the liver and is present in plasma and extracellular fluids. Plasminogen is the inactive precursor of plasmin, a potent serine protease involved in the dissolution of fibrin blood clots. Plasminogen can be converted into the active plasmin by plasminogen activators urokinase (uPA), tissue plasminogen activator (tPA), factor XII-dependent components. The plasmin system has been implicated in a variety of physiological and pathological processes such as fibrinolysis, tissue remodeling, cell migration, inflammation, and tumor invasion and metastasis. Hereditary defects of plasminogen are a predisposing risk factor for thromboembolic disease. |
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| UniProt ID: | P00747 |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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