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Most sensitive ELISA, detecting as little as 0.38 ng/ml of human troponin I
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Flexible sample types, including: cell lysates, plasma, and serum
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Simple protocol with results in just 3.5 hours
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Quantitative results as compared to Western blot analysis
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Low cross reactivity to similar molecules
The Troponin I (human) ELISA kit is a complete, colorimetric, immunometric immunoassay kit for the quantitative determination of human troponin I in cell lysate, plasma, and serum samples with results in about 3.5 hours.
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Product Specification
| Sensitivity: | <0.38 ng/ml (range 0.38 - 25 ng/ml) |
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| Assay Time: | ~3.5 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of human troponin I in cell lysate, plasma, and serum samples. |
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| Wavelength: | 450 nm |
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| Species reactivity: | Human
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| Crossreactivity: | No cross reactivity to similar molecules (cardiac troponin T and cardiac troponin C). There is slight cross-reactivity to muscle troponin I. |
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| Handling: | All kit components are stable at 2 to 8°C. Any unused reconstituted standard should be discarded or frozen at -70°C. Standard can be frozen and thawed one time only without loss of immunoreactivity. |
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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| Contents: | Microtiter Plate, Washing Buffer, Protein Standard, Dilution Buffers, Secondary Antibody, AV-HRP, TMB Substrate, Stop Solution |
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| Scientific Background: | Troponin complex consists of three components: troponin I, T and C. The complex, along with tropomyosin, is located on the actin filament and is essential for the calcium-mediated regulation of skeletal and cardiac muscle contraction. Three isoforms of troponin I have been described for striated muscle. Two isoforms are characteristic for fast and slow skeletal fibers and one isoform for cardiac muscle. Troponin I consists of 181-211 amino acid residues, and the cardiac isoform is larger due to the presence of an additional N-terminal peptide which has a biologically important function in the interactions of troponin I and troponin C. The main function of troponin I is the inhibition of actomyosin ATPase activity. Cardiac troponin I and T are sensitive biomarkers of myocardial injury and have become central to the diagnosis of myocardial infarction. They are elevated in many clinical syndromes associated with direct myocardial injury, myocardial ischemia, or ventricular strain. They are also released in a number of clinical situations in which thrombotic complications of coronary artery disease. These situations include conditions like pulmonary embolism, sepsis, myocarditis, and acute stroke. |
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| UniProt ID: | P19429 |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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