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IL-12p70 (human), ELISA kit

ADI-900-202 96 wells Inquire for pricing
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  • Specific - no cross-reactivity to p40 subunit
  • Sensitive - reliably measure as little as 0.9pg/ml of IL-12p70
  • Non-radioactive - enzymatic assay format is safe and reliable
  • Easy-to-use - liquid color-coded reagents reduce errors
  • Ready-to-use - reagents and pre-coated plates reduce errors and save your time
The IL-12p70 (human), EIA kit is a colorimetric immunometric immunoassay kit with results in 3 hours.
IL-12p70 (human), ELISA kit image
IL-12p70 (human), ELISA kit Parallelism curve
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IL-12p70 (human), ELISA kit image IL-12p70 (human), ELISA kit Parallelism curve

Product Details

Alternative Name:Interleukin-12p70
Sensitivity:0.9 pg/ml (range 7.81 - 500 pg/ml)
Assay Time:3 hours
Applications:ELISA, Colorimetric detection
Application Notes:For the quantitative determination of human IL-12p70 in culture supernatants, plasma, and serum samples.
Wavelength:450 nm
Species reactivity:Human
Crossreactivity:Human IL-12p70 (100%), and ≥0.1%: human IL-23, IL-10, IL-17, IL-13, IL-21, IL-12p40, IL-2, IL-15, IL-29; mouse IL-12
Quantity:1 x 96-well plate
Use/Stability:Store all components at +4ºC, except Standard at -20ºC.
Shipping:Dry Ice and Blue Ice
Short Term Storage:-20°C
Long Term Storage:-20°C
Contents:Microtiter Plate, Conjugate, Antibody, Assay Buffer 28, Wash Buffer Concentrate, Standard, TMB Substrate, Stop Solution 2
Scientific Background:Interleukin-12 (IL-12) belongs to the IL-12 cytokine family, which includes IL-12, IL-23, IL-27, and IL-35. IL-12, a disulfide-linked 70 kDa protein composed of the subunits p40 and p30, is primarily produced by antigen-presenting cells (APCs), such as macrophages, monocytes, and dendritic cells (DCs), following their activation by recognition of pathogen-specific patterns. IL-12 induces secretion of IFN-γ and promotes T-helper 1 (Th1) cell proliferation and differentiation.
UniProt ID:P29459 (IL-12A), P29460 (IL-12B)
Regulatory Status:RUO - Research Use Only
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

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Product Literature References

Exosomal circSHKBP1 participates in non-small cell lung cancer progression through PKM2-mediated glycolysis: W. Chen, et al.; Mol. Ther. Oncolytics 24, 470 (2022), Abstract;

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