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IL-17A (human), ELISA kit

ADI-900-177 96 wells Inquire for pricing
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  • Ultra-Sensitive - detect as little as 0.201 pg/ml
  • Specific - negligible reactivity with other family members
  • Time Savings - results in just 3 hours!
  • Ready-to-use - pre-coated plates and liquid color-coded reagents reduce errors
A highly  sensitive competitive immunoassay for the measurement of IL-17A in human culture supernatants, serum and plasma samples. Results from up to 40 duplicate samples are read on a colorimetric plate reader at 450nm in just 3 hours.
IL-17A (human), ELISA kit Parallelism curve
Parallelism of the curves demonstrates that the antigen binding characteristics are similar enough to allow the accurate determination of native analyte levels in diluted samples of human origin.
IL-17A (human), ELISA kit Standard curve
Dose response curve of recombinant human IL-17A standard.
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IL-17A (human), ELISA kit Parallelism curve IL-17A (human), ELISA kit Standard curve

Product Details

Alternative Name:Interleukin-17A, CTLA-8, Cytotoxic T-lymphocyte-associated antigen 8
Sensitivity:0.2pg/ml (range 2.34-75pg/ml)
Assay Time:3 hours
Applications:ELISA, Colorimetric detection
Application Notes:For the quantitative determination of human IL-17A in culture supernatants, plasma, and serum samples.
Wavelength:450 nm
Species reactivity:Human
Crossreactivity:Human IL-17A (100%) and <0.01%: human IL-17C and IL-17F
Quantity:1 x 96-well plate
Shipping:Dry Ice and Blue Ice
Short Term Storage:-20°C
Long Term Storage:-20°C
Contents:Microtiter plate, Assay buffer 13, Standard, Antibody, Conjugate, Wash buffer concentrate, TMB substrate, Stop solution 2
Scientific Background:IL-17A is produced as a disulfide-linked homodimer that can also form heterodimers with IL-17F. IL-17 acts via ubiquitously expressed IL-17 receptors, inducing expression of pro-inflammatory factors such as IL-1, IL-6, TNF, CXCL8/IL-8, and matrix metalloproteinases. Elevated levels of IL-17A and increased presence of Th17 cells has been associated with airway inflammation, rheumatoid arthritis, intraperitoneal abscesses, inflammatory bowel disease, allograft rejection, psoriasis, multiple sclerosis, and cancer.
Technical Info/Product Notes:Cited samples:
Cytokine ELISAs Cited Samples
UniProt ID:Q16552
Regulatory Status:RUO - Research Use Only
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

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Product Literature References

Vitamin D supplementation for patients with multiple sclerosis treated with interferon-beta: a randomized controlled trial assessing the effect on flu-like symptoms and immunomodulatory properties: D. Golan, et al.; BMC Neurol. 13, 60 (2013), Application(s): ELISA using human serum, Abstract; Full Text

General Literature References

Th17 cells: from precursors to players in inflammation and infection: A. Awasthi & V.K. Kuchroo; Int. Immunol. 21, 489 (2009), Abstract;
Interleukin-17 production in central nervous system-infiltrating T cells and glial cells is associated with active disease in multiple sclerosis: J.S. Tzartos, et al.; Am. J. Pathol. 172, 146 (2008), Abstract;
A brief history of T(H)17, the first major revision in the T(H)1/T(H)2 hypothesis of T cell-mediated tissue damage: L. Steinman; Nat. Med. 13, 139 (2007), Abstract;
Human TH17 lymphocytes promote blood-brain barrier disruption and central nervous system inflammation: H. Kebir, et al.; Nat. Med. 13, 1173 (2007), Abstract;
Interleukin-17 family members and inflammation: J.K. Kolls & A. Linden; Immunity 21, 467 (2004), Abstract;
Increased expression of interleukin 17 in inflammatory bowel disease: S. Fujino, et al.; Gut 52, 65 (2003), Abstract;
Interleukin-17 family and IL-17 receptors: T.A. Moseley, et al.; Cytokine Growth Factor Rev. 14, 155 (2003), Abstract;
CTLA-8, cloned from an activated T cell, bearing AU-rich messenger RNA instability sequences, and homologous to a herpesvirus saimiri gene: E. Rouvier, et al.; J. Immunol. 150, 5445 (1993), Abstract;

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