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17β-Estradiol high sensitivity ELISA kit

Highly sensitive ELISA, detecting as little as 14 pg/ml of 17β-Estradiol
ADI-900-174 96 wells 398.00 USD
Do you need bulk/larger quantities?
Alternative size available: ADI-901-174 (5x96 wells)
  • Highly sensitive measurement, as little as 14 pg/ml
  • Fast and reliable results from up to 37 samples in duplicate in just 3 hours
  • Fully quantitative results that surpass semi-quantitative Western blot analysis
  • Convenient pre-coated plate and color-coded reagents reduces errors
The 17β-Estradiol (serum/plasma) EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 3 hours.
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Product Details

Alternative Name:Estrogen
Sensitivity:14.0 pg/ml (range 15.6 - 1,000 pg/ml)
Assay Time:3 hours
Applications:ELISA, Colorimetric detection
Application Notes:For the quantitative determination of 17β-Estradiol in plasma and serum from any species.
Wavelength:405 nm
Species reactivity:Species independent
Crossreactivity:17β-Estradiol (100%), Estrone (17.8%), Estriol (0.9%), DHT (0.12%), Ethynylestradiol-7α (0.043%), Dehydroisoandroesterone (0.38%), Estradiol-17α (0.032%), Testosterone (0.01%), Progesterone (<0.001%), Danazol (<0.001%)
Use/Stability:The Estradiol-17β Antibody and Conjugate are stable at -20°C. All other components of this kit are stable at +4°C until the kit’s expiration date. The Antibody and Conjugate should be stored at -20°C upon receipt.
Shipping:Blue Ice
Contents:DxS IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
Scientific Background:Biologically active estrogen, 17β-estradiol, is an important sex hormone, but also makes an important contribution to the high concentrations of estrogens, which are present in malignant breast tissues.
Technical Info/Product Notes:Cited samples:
Wildlife Endocrinology: Cited Sample Types
Regulatory Status:RUO - Research Use Only
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

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Product Literature References

17 b ethinylestradiol modulates endothelial function in ovariectomized rat carotid arteries: F. Yuan, et al.; Eur. J. Pharmacol. 944, 175525 (2023), Abstract;
Estrogen and progesterone receptors and antioxidant enzymes are expressed differently in the uterus of domestic cats during the estrous cycle: A.E. de Jesus Nascimento, et al.; Theriogenology 203, 1 (2023), Abstract;
Characterizing seasonal transitions: Breeding-like morphology and behavior during the late non-breeding season in green anole lizards: C.Y. Tao, et al.; Horm. Behav. 139, 105106 (2022), Abstract;
Exposure to a mixture of per-and polyfluoroalkyl substances modulates pulmonary expression of ACE2 and circulating hormones and cytokines: Z. Yang, et al.; Toxicol. Appl. Pharmacol. 4561, 116284 (2022), Abstract;
Structural and functional changes in rat uterus induced by neonatal androgenization: R.C. Genaro, et al.; J. Mol. Histol. 53, 903 (2022), Abstract;
Expression profile of the Kisspeptin/Kiss1r system and angiogenic and immunological mediators in the ovary of cyclic and pregnant cats: L.C. Santos, et al.; Domest. Anim. Endocrinol. 78, 106650 (2021), Abstract;
Gender Differences in Low-Molecular-Mass-Induced Acute Lung Inflammation in Mice: Y. Xie, et al.; Int. J. Mol. Sci. 22, 419 (2021), Abstract;
Neonatal androgenization in rats affects oocyte maturation: G. Anesetti & R. Chávez-Genaro; Reprod Sci. 28, 2799 (2021), Abstract;
Tri-ortho-cresyl phosphate exposure leads to low egg production and poor eggshell quality via disrupting follicular development and shell gland function in laying hens: J. Li, et al.; Ecotoxicol. Environ. Saf. 225, 112771 (2021), Abstract;
Urinary equol levels are positively associated with urinary estradiol excretion in women: T. Fujitani, et al.; Sci. Rep. 11, 19532 (2021), Abstract;
Neuron-Derived Estrogen is Critical for Astrocyte Activation and Neuroprotection of the Ischemic Brain: Y. Lu, et al.; J. Neurosci. 40, 7355 (2020), Abstract; Full Text
Variation in female reproductive tract morphology across the reproductive cycle in the zebra finc: L.L. Hurley, et al.; PeerJ 8, e10195 (2020), Abstract; Full Text
Vulnerability to psychological stress-induced anorexia in female mice depends on blockade of ghrelin signal in nucleus tractus solitarius: C. Yamada, et al.; Br. J. Pharmacol. 177, 4666 (2020), Application(s): Mouse plasma, Abstract; Full Text
Changes in processes downstream of the hypothalamus are associated with seasonal follicle development in a songbird, the dark-eyed junco (Junco hyemalis): K.B. Needham, et al.; Gen. Comp. Endocrinol. 270, 103 (2019), Application(s): ELISA using bird plasma, Abstract;
Effects of 17β-estradiol on colorectal cancer development after azoxymethane/dextran sulfate sodium treatment of ovariectomized mice: C.H. Song, et al.; Biochem. Pharmacol. 164, 139 (2019), Application(s): ELISA using mouse serum, Abstract;
Neuron-derived estrogen regulates synaptic plasticity and memory: Y. Lu, et al.; J. Neurosci. 39, 2792 (2019), Application(s): ELISA using mouse serum and tissue homogenates, Abstract; Full Text
Onset of daily activity in a female songbird is related to peak-induced estradiol levels: J.L. Graham, et al.; Integr. Comp. Biol. 59, 1059 (2019), Application(s): ELISA using bird plasma, Abstract;
Stimulation of nicotinic receptors in the suprachiasmatic nucleus results in a higher number of growing follicles and ova shed: E. Vieyra, et al.; Exp. Physiol. 104, 1179 (2019), Application(s): ELISA using rat serum, Abstract;
Evidence for condition mediated trade-offs between the HPA-and HPG-axes in the wild zebra finch: O.L. Crino, et al.; Gen. Comp. Endocrinol. 259, 189 (2018), Application(s): Aviary Blood Samples, Abstract;
OXTR overexpression leads to abnormal mammary gland development in mice: D. Li, et al.; J. Endocrinol. 239, 121 (2018), Application(s): ELISA using mouse serum, Abstract;
The consequences of porcine IVM medium supplementation with follicular fluid become reflected in embryo quality, yield and gene expression patterns: P. Pawlak, et al.; Sci. Rep. 8, 15306 (2018), Abstract; Full Text
Aromatase changes in depression: A postmortem and animal experimental study: J.L. Wu, et al.; Psychoneuroendocrinology 77, 56 (2017), Application(s): ELISA using rat plasma, Abstract;
Effects of resistance training and estrogen replacement on adipose tissue inflammation in ovariectomized rats: M.F.C. Rodrigues, et al.; Appl. Physiol. Nutr. Metab. 42, 605 (2017), Application(s): ELISA using rat serum, Abstract;
In vivo transplantation of 3D encapsulated ovarian constructs in rats corrects abnormalities of ovarian failure: S. Sittajody, et al.; Nat. Commun. 8, 1858 (2017), Abstract; Full Text
Influence of sexual hormones on neural orofacial perception: B. Alves, et al.; Pain Med. 18, 1549 (2017), Application(s): ELISA using human saliva, Abstract;
Loss of predator aversion in female rats after Toxoplasma gondii infection is not dependent on ovarian steroids: S. Abdulai-Saiku & A. Vyas; Brain Behav. Immun. 65, 95 (2017), Application(s): ELISA using male rat samples with T. Gondi infection , Abstract;
Progesterone Modifies the Responsivity of the Amygdala-mPFC Connection in Male but not Female Wistar Rats: C.M. Contreras, et al.; Neurosci. Lett. 649, 1 (2017), Application(s): ELISA using rat plasma samples, Abstract;
Does BPA alter steroid hormone synthesis in human granulosa cells in vitro?: A. Mansur, et al.; Hum. Reprod. 31, 1562 (2016), Application(s): Medium supernatant from human mural granulosa cells, Abstract;
Effects of Aqueous Extracts of Cynanchum wilfordii in Rat Models for Postmenopausal Hot Flush: G. Lee, et al.; Prev. Nutr. Food Sci. 21, 373 (2016), Abstract; Full Text
GLUT2 proteins and PPARγ transcripts levels are increased in liver of ovariectomized rats: reversal effects of resistance training: L.M. Tomaz, et al.; J. Exerc. Nutrition Biochem. 20, 51 (2016), Application(s): Measurement of 17β-estradiol plasma levels, Abstract; Full Text
Resistance training and ovariectomy: Antagonic effects in mitochondrial biogenesis markers in rat skeletal muscle: M.R. Barbosa, et al.; Int. J. Sports. Med. 37, 841 (2016), Application(s): ELISA using rat serum, Abstract;
A CYP19 Based Sex Determination and Monosex Production in Aquaculture Species Oreochromis niloticus L. and a Cyprinid Cyprinus carpio L.: A.K. Singh, et al.; Fish. Aquac. J. 6, 1 (2015), Application(s): ELISA using fish serum, Full Text
Changes in maternal body composition and metabolism of dairy goats during pregnancy: D. S. Castagnino, et al.; R. Bras. Zootec. 44, 92 (2015), Application(s): ELISA using goat plasma, Full Text
Effect of pentobarbital and isoflurane on acute stress response in rat: X.Y. Wu, et al.; Physiol. Behav. 145, 118 (2015), Application(s): ELISA kit using rat plasma, Abstract;
Effects of bird-feeding activities on the health of wild birds: T.E. Wilcoxen, et al.; Conserv. Physiol. 3, cov058 (2015), Abstract; Full Text
Positive association between serum silicon levels and bone mineral density in female rats following oral silicon supplementation with monomethylsilanetriol: R. Jugdaohsingh, et al.; Osteoporos. Int. 26, 1405 (2015), Application(s): ELISA, Abstract;
The early evolving sex hormone environment is associated with significant outcome and inflammatory response differences after injury: S.J. Zolin, et al.; J. Trauma Acute Care Surg. 78, 451 (2015), Application(s): ELISA kit using human samples, Abstract;
Actions of oestradiol and progesterone on the prostate in female gerbils: reversal of the histological effects of castration: M. Zanatelli, et al.; Reprod. Fertil. Dev. 26, 540 (2014), Abstract;
Brain-derived estrogen exerts anti-inflammatory and neuroprotective actions in the rat hippocampus: Q.G. Zhang, et al.; Mol. Cell. Endocrinol. 389, 84 (2014), Application(s): ELISA kit using rat tissue lysate, Abstract;
Estradiol improves cardiovascular function through up-regulation of SOD2 on vascular wall: Z. Liu, et al.; Redox Biol. 3, 88 (2014), Application(s): ELISA kit using mouse plasma, Abstract; Full Text
Gender differences in adenine-induced chronic kidney disease and cardiovascular complications in rats: V. Diwan, et al.; Am. J. Physiol. Renal Physiol. 307, F1169 (2014), Application(s): ELISA kit using rat samples, Abstract;
Genistein exposure during the early postnatal period favors the development of obesity in female, but not male rats: R.S. Strakovsky, et al.; Toxicol. Sci. 138, 161 (2014), Application(s): ELISA kit using rat serum, Abstract; Full Text
Chronic exposure to benzyl butyl phthalate (BBP) alters social interaction and fear conditioning in male adult rats: alterations in amygdalar MeCP2, ERK1/2 and Erα: A. Betz, et al.; Neuro. Endocrinol. Lett. 34, 347 (2013), Application(s): ELISA kit using rat serum, Abstract;
Cystic ovarian follicles and thyroid activity in the dairy cow: M. Mutinati, et al.; Anim. Reprod. Sci. 138, 150 (2013), Application(s): EIA using bovine serum, Abstract;
Gender-dimorphic regulation of skeletal muscle proteins in streptozotocin-induced diabetic rats: M. Choi, et al.; Cell Physiol. Biochem. 31, 408 (2013), Application(s): EIA using rat plasma, Abstract; Full Text
Songbird frequency selectivity and temporal resolution vary with sex and season: M.D. Gall, et al.; Proc. Biol. Sci. 280, 20122296 (2013), Application(s): ELISA kit using bird plasma, Abstract; Full Text
The screening of everyday life chemicals in validated assays targeting the pituitary-gonadal axis: H. Tinwell, et al.; Regul. Toxicol. Pharmacol. 66, 184 (2013), Application(s): EIA using rat plasma, Abstract;
Effects of velvet antler with blood on bone in ovariectomized rats: S.H. Tseng, et al.; Molecules 17, 10574 (2012), Application(s): ELISA using ether extract of velvet antler, Abstract; Full Text

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