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Highly sensitive with measurement as little as 4.7 pg/ml of aldosterone
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Negligible reactivity with other steroids
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Validated and tested with human and rodent serum and plasma samples
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Fully quantitative results that surpass semi-quantitative Western blots
The Aldosterone EIA kit is a colorimetric competitive enzyme immunoassay kit with results overnight + 1 hour.
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Product Details
| Sensitivity: | 4.7 pg/ml (range 3.9 - 250 pg/ml) |
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| Assay Time: | Overnight + 1 hour |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of Aldosterone in plasma, serum, and urine from any species. |
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| Wavelength: | 405 nm |
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| Species reactivity: | Species independent
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| Crossreactivity: | Aldosterone (100%), 11-Deoxycorticosterone (0.3%), Corticosterone (0.19%), Progesterone (0.20%), and <0.001%: Cortisol, DHT, Estradiol, Testosterone |
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| Use/Stability: | Store all components at -20°C. |
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| Shipping: | Shipped on Blue Ice |
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| Contents: | DxS IgG Microtiter plate, Conjugate, Antibody, Assay buffer concentrate, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution |
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| Scientific Background: | Aldosterone is a steroid hormone synthesized from cholesterol in the adrenal cortex. Aldosterone is metabolized in the kidney and liver, and functions as the key mineralocorticoid in the control of sodium and potassium balance. Synthesis and release of aldosterone by the adrenal gland is primarily regulated by the renin-angiotensin-aldosterone system (RAAS), the main regulatory system involved in blood pressure regulation, renal hemodynamics, and sodium-volume homeostasis. Measurement of serum aldosterone in conjunction with plasma renin is used clinically to differentiate between primary and secondary aldosteronism. Primary aldosteronism (hyperaldosteronism) is characterized by a very low renin:aldosterone ratio leading to the retention of sodium and increased blood pressure, and is typically the result of renal gland hyperplasia or tumors. In secondary aldosteronism, hyperproduction of aldosterone results from external conditions such as heart failure and renal artery disease that reduce renal blood flow and stimulate the RAAS mechanism. The RAAS directly affects vascular and cardiac remodeling through proliferative and inflammatory signaling, as aldosterone and salt have been shown to increase the expression of intracellular cyclooxygenase-2, osteopontin, and MCP-1 in rats. Aldosterone acts by binding to the mineralocorticoid receptor (MR) triggering the transcription of hormone responsive genes, and clinical studies have shown that patients with congestive heart failure or after myocardial infarction benefited from MR antagonist treatment. As pharmacological modulation of nuclear hormone receptors is a common strategy for the treatment of cardiovascular disease, determining the effect of such treatments on the RAAS is of increasing value in evaluating the safety and efficacy of new targeted therapeutics. |
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| Technical Info/Product Notes: | Cited samples:
Hormone ELISAs: Cited Samples
Wildlife Endocrinology: Cited Sample Types |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
A cardiac-null mutation of Prdm16 causes hypotension in mice with cardiac hypertrophy via increased nitric oxide synthase 1: J.O. Kang, et al.; PLoS One
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Abstract;
Effects of amiloride on acetylcholine-dependent arterial vasodilation evolve over time in mice on a high salt diet: S.M. Mutchler, et al.; Physiol. Rep.
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Abstract;
Low-salt diet increases mRNA expression of aldosterone-regulated transporters in the intermediate portion of the endolymphatic sac: A. Matsubara, et al.; Pflugers Arch. (2022),
Abstract;
The microbiota-gut-kidney axis mediates host osmoregulation in a small desert mammal: Z. Nouri, et al.; NPJ Biofilms Microbiomes
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Differential roles of VPS and RAAS in water homeostasis and a risk for kidney dysfunction in rats undergoing rapid fasting/dehydration with regular exercise: K. Hasegawa, et al.; Physiol. Rep.
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Application(s): ELISA using rat plasma,
Abstract;
Full Text
Factors affecting the urinary aldosterone-to-creatinine ratio in healthy dogs and dogs with naturally occurring myxomatous mitral valve disease: A. Galizzi, et al.; BMC Vet. Res.
17, 15 (2021),
Application(s): ELISA using dog urine,
Abstract;
Full Text
Loss of Soluble (Pro)renin Receptor Attenuates Angiotensin-II Induced Hypertension and Renal Injury: N. Ramkumar, et al.; Circ. Res.
129, 50 (2021),
Application(s): Mouse urine and plasma,
Abstract;
Metabolic syndrome during gestation and lactation: An important renal problem in dams. selenium renal clearance: F. Nogales, et al.; J. Trace Elem. Med. Biol.
64, 126709 (2021),
Application(s): ELISA using rat serum,
Abstract;
Renal interstitial fibroblasts coproduce erythropoietin and renin under anaemic conditions: K. Miyauchi, et al.; EBioMedicine
64, 103209 (2021),
Application(s): ELISA using rat plasma,
Abstract;
Full Text
Salt-Sensitivity of Volume and Blood Pressure in a Mouse with Globally Reduced ENaC γ Subunit Expression: E.C. Ray, et al.; Am. J. Physiol. Renal Physiol.
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Abstract;
Sex-dependent Effects of Nephron Ift88 Disruption on Blood Pressure, Renal Function and Cystogenesis: C. Hu, et al.; J. Am. Soc. Nephrol.
32, 2210 (2021),
Abstract;
The Effects of Beverage Intake after Exhaustive Exercise on Organ Damage, Inflammation and Oxidative Stress in Healthy Males: T. Tominaga, et al.; Antioxidants (Basel)
10, 866 (2021),
Application(s): Aldosterone quantification on human plasma samples,
Abstract;
Full Text
The Expression of RAAS Key Receptors, Agtr2 and Bdkrb1, Is Downregulated at an Early Stage in a Rat Model of Wolfram Syndrome: M. Punapart, et al.; Genes (Basel)
12, 1717 (2021),
Abstract;
Differences in Renal BMAL1 Contribution to Na + Homeostasis and Blood Pressure Control in Male and Female Mice: G.R. Crislip, et al.; Am. J. Physiol. Renal Physiol.
318, 1463 (2020),
Abstract;
Full Text
Kisspeptin deficiency leads to abnormal adrenal glands and excess steroid hormone secretion: A. Berthon, et al.; Hum. Mol. Genet.
2020, ddaa215 (2020),
Abstract;
Full Text
Chronic water insufficiency induced kidney damage and energy dysregulation despite reduced food intake, which improved gut microbiota in female rats: J.W. Daily, et al.; J. Physiol. Sci.
69, 599 (2019),
Application(s): Rat Serum,
Abstract;
Effects of Doenjang, a Traditional Korean Soybean Paste, with High-Salt Diet on Blood Pressure in Sprague-Dawley Rats: E.G. Mun, et al.; Nutrients
11, 2745 (2019),
Abstract;
Full Text
Increased salt intake does not worsen the progression of renal cystic disease in high water-loaded PCK rats: S. Nagao, et al.; PLoS One
14, e0207461 (2019),
Application(s): ELISA using rat serum,
Abstract;
Full Text
Salt-deficient diet exacerbates cystogenesis in ARPKD via epithelial sodium channel (ENaC): D.V. Ilatovskaya, et al.; EBioMedicine
40, 663 (2019),
Application(s): ELISA using rat urine,
Abstract;
Full Text
Alteration in microRNA-25 expression regulate cardiac function via renin secretion: H. Li, et al.; Exp. Cell Res.
395, 119 (2018),
Abstract;
Assessment of the Effect of Gasoline Fume on Stress Hormones, Antioxidant Status and Lipid Peroxidation in Albino Rat: F. Owagboriaye, et al.; J. King Saud Univ. Sci. 30, 393 (2018), Application(s): Rat serum samples analyzed,
Hydrogen sulfide attenuates renin angiotensin and aldosterone pathological signaling to preserve kidney function and improve exercise tolerance in heart: Z. Li, et al.; JACC Basic Transl. Sci.
3, 796 (2018),
Application(s): ELISA using mouse kidney,
Abstract;
Full Text
Prorenin independently causes hypertension and renal and cardiac fibrosis in cyp1a1-prorenin transgenic rats: G. Zhou, et al.; Clin. Sci. (Lond.)
132, 1345 (2018),
Abstract;
Full Text
Role of the Renin Angiotensin System in Blood Pressure Allostasis-induced by Severe Food Restriction in Female Fischer rats: A.M.A. de Souza, et al.; Sci. Rep.
8, 10327 (2018),
Abstract;
Full Text
ALD1613, A Novel Long-Acting Monoclonal Antibody to Control ACTH-Driven Pharmacology: A.L. Feldhaus, et al.; Endocrinology
158, 1 (2017),
Abstract;
Effect of renal denervation on urine angiotensinogen excretion in prenatally programmed rats: A. Mansuri, et al.; Physiol. Rep.
5, e13482 (2017),
Abstract;
Full Text
The Relationship Between the Renin-Angiotensin-Aldosterone System and NMDA Receptor-Mediated Signal and the Prevention of Retinal Ganglion Cell Death: M. Kobayashi, et al.; Invest. Ophthalmol. Vis. Sci.
58, 1397 (2017),
Abstract;
Full Text
Effect of Permissive Dehydration on Induction and Decay of Heat Acclimation, and Temperate Exercise Performance: R.A. Neal, et al.; Front. Physiol.
7, 564 (2016),
Abstract;
Full Text
Modulation of the sympathetic nervous system by renal denervation prevents reduction of aortic distensibility in atherosclerosis prone ApoE-deficient rats: M. Hohl, et al.; J. Transl. Med.
14, 167 (2016),
Abstract;
Full Text
Prenatal Testosterone Exposure Decreases Aldosterone Production but Maintains Normal Plasma Volume and Increases Blood Pressure in Adult Female Rats: A.S. More, et al.; Biol. Reprod.
95, 42 (2016),
Application(s): Plasma aldosterone measurements,
Abstract;
Full Text
Thirst responses following high intensity intermittent exercise when access to ad libitum water intake was permitted, not permitted or delayed: S.A. Mears, et al.; Physiol. Behav.
157, 47 (2016),
Application(s): Measured aldosterone in plasma,
Abstract;
Clopidogrel attenuates lithium-induced alterations in renal water and sodium channels/transporters in mice: Y. Zhang, et al.; Purinergic Signal.
11, 507 (2015),
Application(s): Levels of aldosterone in mouse urine,
Abstract;
Effect of renal sympathetic denervation on the progression of paroxysmal atrial fibrillation in canines with long-term intermittent atrial pacing: X. Wang, et al.; Europace
17, 647 (2015),
Application(s): ELISA using dog plasma,
Abstract;
Low TGFβ1 expression prevents and high expression exacerbates diabetic nephropathy in mice: C. K. Hathaway, et al.; PNAS
112, 5815 (2015),
Application(s): ELISA using mouse plasma,
Abstract;
Full Text
Poor Wheel-Running Exercise Can Decrease Blood Pressure through Hormonal Control and Increase Endurance Exercise Capacity in Middle-Aged Normal Rats: S. Sakata, et al.; Journal of Biosciences and Medicines
3, 10 (2015),
Application(s): ELISA using rat serum,
Full Text
Effect of buttermilk consumption on blood pressure in moderately hypercholesterolemic men and women: V. Conway, et al.; Nutrition
30, 116 (2014),
Application(s): ELISA using human plasma,
Abstract;
Methylglyoxal, a reactive glucose metabolite, increases renin angiotensin aldosterone and blood pressure in male Sprague-Dawley rats: I. Dhar, et al.; Am. J. Hypertens.
27, 308 (2014),
Application(s): ELISA using rat plasma,
Abstract;
Myosin phosphatase target subunit 1 (MYPT1) regulates the contraction and relaxation of vascular smooth muscle and maintains blood pressure: Y.N. Qiao, et al.; J. Biol. Chem.
289, 22512 (2014),
Application(s): ELISA using mouse plasma,
Abstract;
Long-term renal denervation normalizes disrupted blood pressure circadian rhythm and ameliorates cardiovascular injury in a rat model of metabolic syndrome: T. Katayama, et al.; J. Am. Heart Assoc.
2, e000197 (2013),
Application(s): ELISA using rat urine,
Abstract;
Full Text
Thyroid hormone is required for hypothalamic neurons regulating cardiovascular functions: J. Mittag, et al.; J. Clin. Invest.
123, 509 (2013),
Application(s): ELISA using mouse serum,
Abstract;
Full Text
Aldosterone induces active K+ secretion by enhancing mucosal expression of Kcnn4c and Kcnma1 channels in rat distal colon: S.K. Singh, et al.; Am. J. Physiol. Cell Physiol.
302, C1353 (2012),
Application(s): ELISA using rat serum,
Abstract;
Full Text
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