- Ultra-sensitive and specific - be confident with your results and use less sample per test
- Quantitative - obtain statistically significant results compared to semi-quantitative Western blot analysis
- Higher throughput - assay up to 40 samples in duplicate in just 3 hours
The [pSer15]HSP27 (human), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours. Save time, money, and precious sample with fully quantitative results and increased sensitivity compared to Western blot analysis.
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Product Details
Alternative Name: | Heat shock protein 27 |
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Sensitivity: | 10.15 pg/ml (range 31.25 - 1000 pg/ml) |
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Assay Time: | 3 hours |
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Applications: | ELISA, Colorimetric detection
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Application Notes: | For the quantitative determination of human [pSer15]HSP27 in cell lysates, plasma, and serum. |
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Wavelength: | 450 nm |
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Species reactivity: | Human
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Crossreactivity: | Phopho HSP27 (Ser15) (100%), HSP27 (non-phospho) (0.885%), HSP70 (0.023%) and <0.016%: HSP25, HSP40, HSP90, αB Crystallin, αA Crystallin |
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Use/Stability: | Store all components at 4°C, except standard and antibody at -20°C. |
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Shipping: | Blue Ice |
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Contents: | Microtiter plate, Conjugate, Antibody, Assay buffer 27, Wash buffer concentrate, Standard, TMB Substrate, 5X Extraction reagent, Stop solution 2 |
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Scientific Background: | Hsp27 is one of the most common members of the highly conserved and ubiquitously expressed family of small heat shock proteins (sHsp), which also includes alphaB-crystallin. It is characterized by a conserved C-terminal alpha-crystallin domain consisting of two anti-parallel beta-sheets that promote oligomer formation required for its primary chaperone function as inhibitor of irreversible protein aggregation. Hsp27 oligomerization is modulated by post-translational phosphorylation of Hsp27 at three serine residues, Ser15, Ser78, and Ser82, by a variety of protein kinases including MAPKAPK-3, PKAc-α, p70 S6K, PKD I, and PKC-δ. Hsp27 has been shown to inhibit actin polymerization by binding of unphosphorylated Hsp27 monomers to actin intermediate filaments. Anti-apoptotic functions of Hsp27 have also been identified through interactions with DAXX7, activation of Akt, and inhibition of apoptosome formation. Evidence suggests altered expression of Hsp27 is implicated in the pathogenesis of breast, ovarian, and prostate cancer. |
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UniProt ID: | P04792 |
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GenBank ID: | L39370 |
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Regulatory Status: | RUO - Research Use Only |
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Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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