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[pSer473/474]Akt1/2 ELISA kit

ADI-900-162 96 wells 584.00 USD
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  • Ultra-Sensitive - measure as little as 5.5 pg/ml Akt1/2 phosphorylated on Ser473/474
  • Fast - fully quantitative results from up to 41 samples in duplicate in just 3 hours
  • Reliable - validated with up/down regulation experiments and compared to Western blot
The [pSer473/474]Akt1/2 EIA kit is a colorimetric immunometric enzyme immunoassay kit. Absorbance is read at 450 nm. This is an ultra-sensitive assay that lets you measure as little as 5.5 pg/mL Akt1 or Akt2 phosphorylated on Ser473 or Ser474. Obtain fully quantitative results from up to 41 samples in duplicate in just 3 hours. The assay has been validated with up and down regulation experiments and compared to Western blot.
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Product Details

Alternative Name:PKB, Protein kinase B
Sensitivity:5.5 pg/ml (range 17.5 - 560 pg/ml)
Assay Time:3 hours
Applications:ELISA, Colorimetric detection
Application Notes:For the quantitative determination of [pSer473/474]Akt1/2 in cell lysates of human, mouse, and rat origin.
Wavelength:450 nm
Species reactivity:Human, Mouse, Rat
Crossreactivity:Phospho Akt1, 2 (~100%), inactive Akt1 (0.05%), inactive p38α (0.17%) and <0.03%: inactive Akt3, inactive Erk1, inactive Erk2, inactive Jnk1
Use/Stability:Store all components at 4°C, except standard at -20°C.
Shipping:Shipped on Blue Ice
Contents:Microtiter plate, Conjugate, Antibody, Assay buffer 32, Wash buffer concentrate, Standard, TMB Substrate, Cell lysis buffer 5, Stop solution 2
Scientific Background:The Akt (PKB) family of protein kinases are serine/threonine kinases, with three mammalian family members identified to date (Akt1, Akt2, Akt3). Akt is a well-characterized member of PI3 kinase-mediated signaling pathways, regulating cell growth, apoptosis, glycogen synthesis, and other cellular responses through its phosphorylation of downstream substrates. Akt activation is triggered by binding of phospholipid and phosphorylation at two key residues: Thr308 by PDK1, and Ser473/474 by PDK2, now identified as mTOR. Deregulation of Akt signaling has been associated with cancer, diabetes, and schizophrenia. Akt1 is the cellular homologue of the murine thymoma retroviral oncogene v-akt, and its role in anti-apoptotic and pro-mitotic pathways have made Akt a molecular target for anti-cancer therapeutic intervention. Akt activation inhibits apoptosis by phosphorylating the Bcl-2 related protein Bad, and increases p53 degradation by phosphorylating mdm2. Mitotic substrates of Akt include GSK-3β, p21CIP1, and p27KIP1, cell cycle inhibitors negatively regulated by Akt phosphorylation. Akt has been shown to mediate angiogenesis through regulation of thrombospondins, which may cooperate with pro-mitotic and anti-apoptotic functions of Akt to promote tumorigenesis.
UniProt ID:P31749 (human Akt1), P31751 (human Akt2)
Regulatory Status:RUO - Research Use Only
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

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Product Literature References

SEPT9 negatively regulates ubiquitin-dependent downregulation of EGFR: K. Diesenberg, et al.; J. Cell Sci. 128, 397 (2015), Application(s): HeLa Cell Lysates, Abstract; Full Text

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