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Glutathione (GSSG/GSH), detection kit

Rapid, ultra-sensitive kit for the quantification of reduced (GSH), oxidized (GSSG), and total glutathione for oxidative stress research.
ADI-900-160 4x96 wells 434.00 USD
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  • Highly sensitive enzymatic recycling reaction measures total, oxidized, or reduced glutathione
  • Easy-to-use sample handling protocols for a wide variety of matrices
  • Convenient and colorimetric 96 well plate format
The Glutathione (GSSG/GSH), detection kit provides a highly sensitive enzymatic recycling reaction for the measurement of total, oxidized, or reduced glutathione in a convenient 96-well plate format.

Product Specification

Sensitivity:6.25 pmoles/well (range 12.5 - 100 pmoles/well)
Applications:Colorimetric detection
Application Notes:For the measurement of total, oxidized and reduced forms of glutathione in cell lysates, plasma, saliva, tissue, urine, and whole blood from any species.
Shipping:Blue Ice Not Frozen
Long Term Storage:+4°C
Contents:Microtiter plate, Assay buffer concentrate, Reaction mix, Glutathione reductase, GSSG
Scientific Background:Glutathione, a major intracellular non-protein thiol, protects against free radical damage by providing reducing equivalents for several key antioxidant enzymes. In addition, glutathione acts as a scavenger of hydroxyl radicals and nascent oxygen. Elevated glutathione levels are associated with a reduced rate of illness, higher levels of self-rated health, lower cholesterol, lower body mass index, and lower blood pressure among the elderly. Glutathione provides a primary defense system for the removal of oxidants in the brain. Studies have revealed a correlation between low glutathione levels and damage to neurons that manufacture dopamine, suggesting a link to Parkinson's disease.
Regulatory Status:RUO - Research Use Only

Product Literature References

Environmental Enrichment Attenuates Oxidative Stress and Alters Detoxifying Enzymes in an A53T α-Synuclein Transgenic Mouse Model of Parkinson's Disease: J.H. Seo, et al.; Antioxidants (Basel) 9, 928 (2020), Abstract;
Melatonin Prevents Transforming Growth Factor-β1-Stimulated Transdifferentiation of Renal Interstitial Fibroblasts to Myofibroblasts by Suppressing Reactive Oxygen Species-Dependent Mechanisms: J.Y. Kim, et al.; Antioxidants 9, 39 (2020), Abstract;
Pentacyclic triterpenoid-rich fraction of the Hardy kiwi (Actinidia arguta) improves brain dysfunction in high fat diet-induced obese mice: J.S. Ha, et al.; Sci. Rep. 10, 5788 (2020), Abstract; Full Text
A Diet Enriched in Palmitate and Deficient in Linoleate Exacerbates Oxidative Stress and Amyloid-β Burden in the Hippocampus of 3xTg-AD Mouse Model of Alzheimer's Disease: G. Marwarha, et al.; J. Alzheimers Dis. 68, 219 (2019), Application(s): Mouse hippocampal tissue homogenates, Abstract;
A Diet Enriched in Palmitate and Deficient in Linoleate Exacerbates Oxidative Stress and Amyloid-β Burden in the Hippocampus of 3xTg-AD Mouse Model of Alzheimer’s Disease: G. Marwarha, et al.; J. Alzheimers Dis. 68, 219 (2019), Abstract;
A phospholipase D2 inhibitor, CAY10594, ameliorates acetaminophen-induced acute liver injury by regulating the phosphorylated-GSK-3β/JNK axis: S.K. Lee, et al.; Sci. Rep. 9, 7242 (2019), Abstract; Full Text
Excess homocysteine upregulates the NRF2-antioxidant pathway in retinal Müller glial cells: S. Navneet, et al.; Exp. Eye Res. 178, 228 (2019), Abstract;
Urine and plasma concentrations of amino acids and plasma vitamin status differ, and are differently affected by salmon intake, in obese Zucker fa/fa rats with impaired kidney function and in Long-Evans rats with healthy kidneys: A. Drotningsvik, et al.; Br. J. Nutr. 9, 1 (2019), Application(s): Measurement of rat plasma glutathione, Abstract;
Bile Acids Increase Doxorubicin Sensitivity in ABCC1-expressing Tumour Cells: S. Chewchuk, et al.; Sci. Rep. 8, 5413 (2018), Abstract; Full Text
Ethyl acetate fraction from Hibiscus sabdariffa L. attenuates diabetes-associated cognitive impairment in mice: T.W. Seung, et al.; Food Res. Int. 105, 589 (2018), Application(s): Whole brain tissues, Abstract; Full Text
Heterozygous huntingtin promotes cadmium neurotoxicity and neurodegeneration in striatal cells via altered metal transport and protein kinase C delta dependent oxidative stress and apoptosis signaling mechanisms: G.F. Kwakye, et al.; Neurotoxicology 70, 48 (2018), Abstract;
Oxidative stress in elite athletes training at moderate altitude and at sea level: J. Leon-Lopez, et al.; Eur. J. Sport Sci. 18, 832 (2018), Abstract;
Activated protein C protects against pressure overload-induced hypertrophy through AMPK signaling: C. Cates, et al.; Biochem. Biophys. Res. Commun. 495, 2584 (2017), Application(s): C57BL/6J and AMPK kinase heart tissue , Abstract;
Acute exposure to chlorpyrifos caused NADPH oxidase mediated oxidative stress and neurotoxicity in a striatal cell model of Huntington's disease: G.A. Dominah, et al.; Neurotoxicology 60, 54 (2017), Abstract;
Acute exposure to chlorpyrifos caused NADPH oxidase mediated oxidative stress and neurotoxicity in a striatal cell model of Huntington’s disease: G.A. Dominah, et al.; Neurotoxicology 60, 54 (2017), Abstract;
Antioxidant Treatment in Male Mice Prevents Mitochondrial and Synaptic Changes in an NMDA Receptor Dysfunction Model of Schizophrenia: A. Phensy, et al.; eNeuro 4, ENEURO.0081-17.2017 (2017), Abstract; Full Text
Cellular mechanisms of action and resistance of Plasmodium falciparum to artemisinin: P. Phompradit, et al.; Parasitol. Res. 116, 3331 (2017), Abstract;
Hypoxic Air Inhalation and Ischemia Interventions Both Elicit Preconditioning Which Attenuate Subsequent Cellular Stress In vivo Following Blood Flow Occlusion and Reperfusion: J.H. Barrington, et al.; Front. Physiol. 8, 850 (2017), Abstract; Full Text
Low-density lipoprotein docosahexaenoic acid nanoparticles induce ferroptotic cell death in hepatocellular carcinoma: W. Ou, et al.; Free Radic. Biol. Med. 112, 597 (2017), Abstract;
Nigella sativa seed extract attenuates the fatigue induced by exhaustive swimming in rats: M. Rahman, et al.; Biomed. Rep. 6, 468 (2017), Abstract; Full Text
Reduced Slc1a1 expression is associated with neuroinflammation and impaired sensorimotor gating and cognitive performance in mice: Implications for schizophrenia: P. Afshari, et al.; PLoS One 12, e01083854 (2017), Abstract; Full Text
α-Synuclein Enhances Cadmium Uptake and Neurotoxicity via Oxidative Stress and Caspase Activated Cell Death Mechanisms in a Dopaminergic Cell Model of Parkinson's Disease: W. Chong, et al.; Neurotox. Res. 32, 231 (2017), Abstract;
Ethanol Extract of Cirsium japonicum var. ussuriense Kitamura Exhibits the Activation of Nuclear Factor Erythroid 2-Related Factor 2-dependent Antioxidant Response Element and Protects Human Keratinocyte HaCaT Cells Against Oxidative DNA Damage: O.K. Yoo, et al.; J. Cancer Prev. 21, 66 (2016), Application(s): Determination of intracellular reduced glutathione level, Abstract; Full Text
Novel triterpenoid AECHL-1 induces apoptosis in breast cancer cells by perturbing the mitochondria-endoplasmic reticulum interactions and targeting diverse apoptotic pathways: M.A. Sawant, et al.; Biochim. Biophys. Acta 1860, 1056 (2016), Application(s): Detection of total GSH levels in vivo, Abstract;
Prominent role of DPP III in the E2-mediated protection against hyperoxia in liver of female CBA/H mice: S. Sobočanec , et al.; Redox Biol. 8, 149 (2016), Application(s): Determination of glutathione level in liver cells of mice, Abstract;
Protection of Chinese olive fruit extract and its fractions against advanced glycation endproduct-induced oxidative stress and pro-inflammatory factors in cultured vascular endothelial and human monocytic cells: C.T. Kuo, et al.; J. Funct. Foods 27, 526 (2016), Application(s): EA.hy926 cells ,
Red bean extracts protect rats against intestinal inflammatory damage: K.C. Choi, et al.; Food Sci. Biotechnol. 25, 349 (2016), Abstract; Full Text
Ameliorating effects of ethyl acetate fraction from onion (Allium cepa L.) flesh and peel in mice following trimethyltin-induced learning and memory impairment: S. K. Park, et al.; Food Res. Int. 75, 53 (2015), Application(s): Total glutathione measured in mouse brain tissue homogenate, Abstract;
Antiamnesic Effect of Actinidia arguta Extract Intake in a Mouse Model of TMT-Induced Learning and Memory Dysfunction: J.S. Ha, et al.; Evid. Based Complement. Alternat. Med. Article ID 876484 (2015), Application(s): Determination of GSH and oxidized glutathione dimer in mouse brain, Abstract;
C/EBPγ is a critical regulator of cellular stress response networks through heterodimerization with ATF4: C.J. Huggins, et al.; Mol. Cell. Biol. 36, 693 (2015), Abstract; Full Text
Catechin-7-O-β-D-glucopyranoside isolated from the seed of Phaseolus calcaratus Roxburgh ameliorates experimental colitis in rats: S.H. Kook, et al.; Int. Immunopharmacol. 29, 521 (2015), Abstract;
Celastrol prevents circulatory failure via induction of heme oxygenase-1 and heat shock protein 70 in endotoxemic rats: Y.L. Wang, et al.; J. Ethnopharmacol. 162, 168 (2015), Application(s): Assay, Abstract;
Evaluation of gene expression changes in human primary lung epithelial cells following 24-hr exposures to inorganic arsenic and its methylated metabolites and to arsenic trioxide: A.Y. Efremenko, et al.; Environ. Mol. Mutagen. 56, 477 (2015), Abstract;
Mancozeb affects mitochondrial activity, redox status and ATP production in mouse granulosa cells: R. Iorio, et al.; Toxicol. In Vitro 30, 438 (2015), Abstract;
Effect of the combination of metformin hydrochloride and melatonin on oxidative stress before and during pregnancy, and biochemical and histopathological analysis of the livers of rats after treatment for polycystic ovary syndrome: A.J. Lemos, et al.; Toxicol. Appl. Pharmacol. 14, 212 (2014), Abstract;
The Chinese herbal formula Liuwei dihuang protects dopaminergic neurons against Parkinson's toxin through enhancing antioxidative defense and preventing apoptotic death: Y.T. Tseng, et al.; Phytomedicine 21, 724 (2014), Application(s): Total GSH measured in rat primary mesencephalic neuron lysates, Abstract;
Establishing the thermal threshold of the tropical mussel Perna viridis in the face of global warming: B.P. Goh & C.H. Lai; Mar. Pollut. Bull. 13, 662 (2013), Abstract;
Insulin-like growth factor I (IGF-I)-induced chronic gliosis and retinal stress lead to neurodegeneration in a mouse model of retinopathy: P. Villacampa, et al.; J. Biol. Chem. 288, 24 (2013), Abstract; Full Text
Continuous presence of H₂O₂ induces mitochondrial-mediated, MAPK- and caspase-independent growth inhibition and cytotoxicity in human gingival fibroblasts: J.Y. Yu, et al.; Toxicol. In Vitro 26, 561 (2012), Application(s): Used with human gingival fibroblasts (HGF) and human YD-38 cell lysates., Abstract;
Continuously generated H2O2 stimulates the proliferation and osteoblastic differentiation of human periodontal ligament fibroblasts: Y. Choe, et al.; J. Cell. Biochem. 113, 1426 (2012), Application(s): Used with human periodontal ligament fibrobast (PLF) lysates., Abstract; Full Text
Elevated circulating angiogenic progenitors and white blood cells are associated with hypoxia-inducible angiogenic growth factors in children with sickle cell disease: S.F. Ofori-Acquah, et al.; Anemia 2012, (2012), Abstract; Full Text
An oxidative environment promotes growth of Mycobacterium abscessus: R.E.Oberley-Deegan, et al.; Free Radic. Biol. Med. 49, 1666 (2010), Application(s): THP-1 cell lysates, Abstract; Full Text

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