Replaces Prod. #: ALX-850-261
- Reliable - fully validated in-house with both cytosolic and mitochondrial cell fractions
- Ready-to-use - cell fractionation reagents provided
- Higher throughput - save time and sample with fully quantitative results and increased sensitivity compared to Western blot
- Fast - results from up to 40 samples in duplicate in ~3 hours
The Cytochrome c (human), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours 15 minutes. Absorbance is read at 405 nm. This kit has been validated in-house with both cytosolic and mitochondrial cell fractions and is supplied with the cell fractionation buffers. Save time, money, and precious sample with fully quantitative results and increased sensitivity compared to Western blot analysis.
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Product Specification
| Sensitivity: | 6.03 pg/ml (range 28.13 - 900 pg/ml) |
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| Assay Time: | 3 hours 15 minutes |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of human Cytochrome c in cell lysates |
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| Wavelength: | 405 nm |
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| Species reactivity: | Human
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| Crossreactivity: | Human Cytochrome c (100%), rat Cytochrome c (6.41%), horse Cytochrome c (0.92%), dog Cytochrome c (0.62%), bovine Cytochrome c (0.61%) and <0.03%: Bax, Bcl-2, Bcl-XL, Caspase-9, cIAP-1, Survivin |
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| Use/Stability: | Store all components at 4°C, except standard and conjugate at -20°C. |
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| Shipping: | Shipped on Blue Ice |
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| Contents: | Microtiter plate, Conjugate, Antibody, Assay buffer 13, RIPA Cell lysis buffer 2, Digitonin cell permeabilization buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution |
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| Scientific Background: | Cytochrome c, which is a critical component of the electron transport chain, is a component of the functional apoptosome. Upon release from the mitochondria, cytochrome c binds to the adaptor proteins Apaf-1 and procaspase-9 to form a cytosolic apoptosome complex. This event results in activation of procaspase-9 and its subsequent cleavage and activation of caspase-3 leading to the degradation of a series of cellular substrates and subsequently cell death. |
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| UniProt ID: | P08574 |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Minocycline an antimicrobial agent attenuates the mitochondrial dependent cell death and stabilizes the expression of HIF-1α in spinal cord injury: G. Zhang, et al.; Microb. Pathog. (2018),
Abstract;
Gamma-tocotrienol acts as a BH3 mimetic to induce apoptosis in neuroblastoma SH-SY5Y cells: J.K. Tan, et al.; J. Nutr. Biochem.
31, 28 (2016),
Application(s): Measured cytochrome c levels in the cytosol and mitochondria,
Abstract;
Norcantharidin induces apoptosis in human prostate cancer cells through both intrinsic and extrinsic pathways: P.Y. Yang, et al.; Pharmacol. Rep.
68, 874 (2016),
Application(s): Used to determine whether cytochrome c was released during norcantharidin-induced apoptosis,
Abstract;
Butein shows cytotoxic effects and induces apoptosis in human ovarian cancer cells: P.Y. Yang, et al.; Am. J. Chin. Med.
43, 769 (2015),
Abstract;
α-Terthienylmethanol, isolated from Eclipta prostrata, induces apoptosis by generating reactive oxygen species via NADPH oxidase in human endometrial cancer cells: J. S. Lee, et al.; J. Ethnopharmacol.
169, 426 (2015),
Application(s): ELISA using human supernatant,
Abstract;
Evidence of oxidative stress and mitochondrial respiratory chain dysfunction in an in vitro model of sepsis-induced kidney injury: C. Quoilin, et al.; Biochim. Biophys. Acta.
1837, 1790 (2014),
Application(s): Quantification of cytochrome c in HK-2 cells,
Abstract;
Pterostilbene induces mitochondrially derived apoptosis in breast cancer cells in vitro: D. Moon, et al.; J. Surg. Res.
180, 208 (2013),
Application(s): MDA-MB231 and MCF-7 human breast cancer cell lysates,
Abstract;
d-Penicillamine targets metastatic melanoma cells with induction of the unfolded protein response (UPR) and Noxa (PMAIP1)-dependent mitochondrial apoptosis: S. Qiao, et al.; Apoptosis
17, 1079 (2012),
Application(s): EIA using metastatic melanoma cells,
Abstract;
Genomic Analysis of Pterostilbene Predicts Its Antiproliferative Effects Against Pancreatic Cancer In Vitro and In Vivo: D.E. McCormack, et al.; J. Gastrointest. Surg.
16, 1136 (2012),
Application(s): Cell lysates from MIA PaCa-2 and PANC-1 cells,
Abstract;
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