- Sensitive - detect picogram levels of protein compared to microgram levels in Western blot
- Reliable - thoroughly validated in-house with a variety of cell lines
- Efficient - save precious sample by using less per test
The Bax (human), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours. Absorbance is read at 450 nm. The Bax (human), EIA kit provides sensitive results - detect picogram levels of protein compared to microgram levels in Western blot and extend the utility of your precious samples.
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Product Details
Alternative Name: | BCL2L4, BCL2-associated X protein |
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Sensitivity: | 25.6 pg/ml (range 62.5 - 2,000 pg/ml) |
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Assay Time: | 3 hours |
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Applications: | ELISA, Colorimetric detection
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Application Notes: | For the quantitative determination of Bax in cell lysates of human origin. |
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Wavelength: | 450 nm |
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Species reactivity: | Human
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Crossreactivity: | Human Bax (100%), p21 (<0.7%) and <0.1%: Bcl-2, Bcl-XL, CDK4, p27, PCNA |
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Use/Stability: | Store all components at 4°C, except standard at -20°C. |
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Shipping: | Blue Ice |
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Contents: | Microtiter plate, Conjugate, Antibody, Assay buffer 13, Cell lysis buffer 4, Wash buffer concentrate, Standard, TMB Substrate, Stop solution 2 |
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Scientific Background: | Bax is an appromimately 22 kDa pro-apoptotic protein belonging to the Bcl-2 family of proteins, which are characterized by the presence of at least one Bcl-2 homology (BH) domains. Bax normally resides in the cytoplasm, but following an apoptotic stimuli, Bax undergoes a conformational change and translocates to mitochondrial membranes where it inserts and mediates the release of cytochrome c. This activates caspase-3 and perpetuates the apoptotic cascade. |
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UniProt ID: | Q07812 |
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Regulatory Status: | RUO - Research Use Only |
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Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Copper oxide nanoparticles promote amyloid-β-triggered neurotoxicity through formation of oligomeric species as a prelude to Alzheimer's diseases: L.A.J. Alhada & M. Falahati; Int. J. Biol. Macromol.
207, 121 (2022),
Abstract;
Ciprofloxacin triggers the apoptosis of human triple-negative breast cancer MDA-MB-231 cells via the p53/Bax/Bcl-2 signaling pathway: A. Beberok, et al.; Int. J. Oncol.
52, 1727 (2018),
Abstract;
Cilostazol Suppresses Aβ-induced Neurotoxicity in SH-SY5Y Cells through Inhibition of Oxidative Stress and MAPK Signaling Pathway: T. Oguchi, et al.; Front. Aging Neurosci.
9, 337 (2017),
Application(s): SH-SY5Y cells lysates,
Abstract;
Full Text
Human blood molecular biodosimeter panel for distinguishing radiation exposure from inflammation stress: A.J. Wyrobek, et al.; (2016), (U. S. Patent),
Full Text
Pterostilbene induces mitochondrially derived apoptosis in breast cancer cells in vitro: D. Moon, et al.; J. Surg. Res.
180, 208 (2013),
Application(s): MDA-MB231 and MCF-7 human breast cancer cell lysates,
Abstract;
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