Bax (human), ELISA kit



ADI-900-138	96 wells	766.00 USD
Do you need bulk/larger quantities?

Sensitive - detect picogram levels of protein compared to microgram levels in Western blot
Reliable - thoroughly validated in-house with a variety of cell lines
Efficient - save precious sample by using less per test

The Bax (human), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours. Absorbance is read at 450 nm. The Bax (human), EIA kit provides sensitive results - detect picogram levels of protein compared to microgram levels in Western blot and extend the utility of your precious samples.

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Product Details

Alternative Name:	BCL2L4, BCL2-associated X protein

Sensitivity:	25.6 pg/ml (range 62.5 - 2,000 pg/ml)

Assay Time:	3 hours

Applications:	ELISA, Colorimetric detection

Application Notes:	For the quantitative determination of Bax in cell lysates of human origin.

Wavelength:	450 nm

Species reactivity:	Human

Crossreactivity:	Human Bax (100%), p21 (<0.7%) and <0.1%: Bcl-2, Bcl-XL, CDK4, p27, PCNA

Use/Stability:	Store all components at 4°C, except standard at -20°C.

Shipping:	Blue Ice

Contents:	Microtiter plate, Conjugate, Antibody, Assay buffer 13, Cell lysis buffer 4, Wash buffer concentrate, Standard, TMB Substrate, Stop solution 2

Scientific Background:	Bax is an appromimately 22 kDa pro-apoptotic protein belonging to the Bcl-2 family of proteins, which are characterized by the presence of at least one Bcl-2 homology (BH) domains. Bax normally resides in the cytoplasm, but following an apoptotic stimuli, Bax undergoes a conformational change and translocates to mitochondrial membranes where it inserts and mediates the release of cytochrome c. This activates caspase-3 and perpetuates the apoptotic cascade.

UniProt ID:	Q07812

Regulatory Status:	RUO - Research Use Only

Compatibility:	This product is compatible with the Absorbance 96 Plate Reader.

Product Literature References

Copper oxide nanoparticles promote amyloid-β-triggered neurotoxicity through formation of oligomeric species as a prelude to Alzheimer's diseases: L.A.J. Alhada & M. Falahati; Int. J. Biol. Macromol. 207, 121 (2022), Abstract;

Ciprofloxacin triggers the apoptosis of human triple-negative breast cancer MDA-MB-231 cells via the p53/Bax/Bcl-2 signaling pathway: A. Beberok, et al.; Int. J. Oncol. 52, 1727 (2018), Abstract;

Cilostazol Suppresses Aβ-induced Neurotoxicity in SH-SY5Y Cells through Inhibition of Oxidative Stress and MAPK Signaling Pathway: T. Oguchi, et al.; Front. Aging Neurosci. 9, 337 (2017), Application(s): SH-SY5Y cells lysates, Abstract; Full Text

Human blood molecular biodosimeter panel for distinguishing radiation exposure from inflammation stress: A.J. Wyrobek, et al.; (2016), (U. S. Patent), Full Text

Pterostilbene induces mitochondrially derived apoptosis in breast cancer cells in vitro: D. Moon, et al.; J. Surg. Res. 180, 208 (2013), Application(s): MDA-MB231 and MCF-7 human breast cancer cell lysates, Abstract;